Detailed information NTG0051054

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7A_RS03275
• Genome accession: NC_017857
• Coordinates: 694828..695970 (+)
• Length: 380 a.a.
• NCBI ID: WP_014705912.1
• Uniprot ID: I1XGL8
• Organism: Methylophaga nitratireducenticrescens
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 689828..700970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7A_RS03250 (Q7A_686)	-	690371..691519 (-)	1149	WP_014705907.1	homoserine O-acetyltransferase	-
Q7A_RS03255 (Q7A_687)	-	691541..692116 (-)	576	WP_014705908.1	YggT family protein	-
Q7A_RS03260 (Q7A_688)	proC	692109..692942 (-)	834	WP_014705909.1	pyrroline-5-carboxylate reductase	-
Q7A_RS03265 (Q7A_689)	-	692991..693689 (-)	699	WP_014705910.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7A_RS03270 (Q7A_690)	pilT	693781..694818 (+)	1038	WP_014705911.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7A_RS03275 (Q7A_691)	pilU	694828..695970 (+)	1143	WP_014705912.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7A_RS03280 (Q7A_692)	-	696143..696994 (+)	852	WP_014705913.1	OmpA family protein	-
Q7A_RS03285 (Q7A_693)	-	697396..697587 (+)	192	WP_014705914.1	CsbD family protein	-
Q7A_RS03290 (Q7A_694)	-	697803..698363 (+)	561	WP_202971549.1	BON domain-containing protein	-
Q7A_RS03295 (Q7A_695)	-	698371..698832 (+)	462	WP_014705916.1	hypothetical protein	-
Q7A_RS03300 (Q7A_696)	-	699053..700279 (+)	1227	WP_014705917.1	sigma-54-dependent Fis family transcriptional regulator	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42218.25 Da
• Isoelectric Point: 5.2278

>NTDB_id=51054 Q7A_RS03275 WP_014705912.1 694828..695970(+) (pilU) [Methylophaga nitratireducenticrescens]
MEIVTILKAAVKHEASDIFITSGRPATLKVNGRMATLSQEPLTEEEARELVLSTMNDEQKRTFERDKECNYAIDTKGAGR
FRVSALYQRSRIAMVLRLVKGEIPTIEELHVPEIIKELAMTKRGLVMFVGATGSGKSTTLAAMIGYRNQNSTGHILTIED
PIEFDHKHAGCVVTQREVGVDTDSYEVALKNSLRQAPDVIMIGEIRSRDTMDYGISFAETGHLCLSTLHANNANQAMERV
INFFPEDRHQQLFLDLSLNLKAVVAQRLIPKKDGSGRAVAVEVLLNTPLIGDLLEKGKVAEIKDVMKRSRELGMQTFDQS
LYDLYKAGEISYEDALRNADSSNEVRLMIKLGSETGELPVDAGAQALSLEMENDPMDSRF

Nucleotide

• Length: 1143 bp

>NTDB_id=51054 Q7A_RS03275 WP_014705912.1 694828..695970(+) (pilU) [Methylophaga nitratireducenticrescens]
ATGGAAATAGTCACTATTCTTAAGGCTGCGGTAAAACACGAAGCCTCTGATATTTTTATTACTTCAGGCCGGCCAGCCAC
CTTGAAAGTGAATGGCCGTATGGCAACGCTTTCGCAGGAACCTTTAACTGAAGAAGAAGCGCGTGAATTGGTATTGAGCA
CCATGAACGACGAACAAAAACGTACTTTTGAACGTGATAAGGAATGTAATTACGCTATTGATACCAAAGGTGCCGGCCGT
TTTAGGGTTAGTGCGCTGTATCAACGCAGTCGAATCGCCATGGTATTACGTTTGGTAAAAGGGGAAATCCCGACCATTGA
AGAGCTGCATGTTCCTGAAATCATCAAAGAGCTTGCTATGACCAAGCGTGGTTTGGTGATGTTTGTGGGGGCCACCGGAA
GTGGTAAGTCAACCACGCTGGCGGCAATGATTGGTTATCGTAATCAGAACAGCACCGGACACATTTTAACGATTGAAGAC
CCGATTGAATTTGATCATAAACATGCAGGATGTGTTGTCACACAGCGAGAAGTGGGGGTGGATACCGACTCCTACGAAGT
CGCATTGAAAAATTCATTACGACAGGCACCGGACGTCATTATGATTGGTGAAATTCGTAGCCGCGACACAATGGATTACG
GTATTTCATTTGCTGAAACCGGTCACCTTTGTTTATCAACCCTTCACGCCAATAATGCTAACCAGGCAATGGAACGGGTG
ATTAACTTTTTCCCGGAAGACCGGCATCAGCAATTATTTCTTGATTTGTCTCTAAACCTTAAAGCTGTTGTGGCACAACG
GCTGATTCCAAAAAAAGATGGTTCCGGGCGGGCGGTTGCAGTGGAAGTTCTGCTCAATACACCACTAATTGGAGATTTGC
TCGAAAAGGGTAAGGTTGCCGAAATCAAGGATGTGATGAAGCGTTCACGGGAATTAGGGATGCAAACGTTCGATCAATCT
TTATATGATTTGTATAAAGCTGGAGAAATCTCATACGAAGACGCATTGCGAAATGCTGATTCCAGTAATGAAGTAAGGTT
GATGATCAAATTAGGATCTGAAACTGGAGAACTCCCTGTGGACGCTGGTGCCCAAGCTTTATCCCTAGAAATGGAAAACG
ATCCTATGGATAGCCGGTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-271)

3D structure

• AlphaFold DB: I1XGL8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.032	98.421	0.611
pilU	Acinetobacter baylyi ADP1	57.459	95.263	0.547
pilU	Vibrio cholerae strain A1552	55.429	92.105	0.511
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.279	90.526	0.374