Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q7C_RS07820 Genome accession   NC_017856
Coordinates   1634984..1636021 (-) Length   345 a.a.
NCBI ID   WP_014704196.1    Uniprot ID   I1YIN3
Organism   Methylophaga frappieri     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629984..1641021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7C_RS07800 (Q7C_1624) - 1630822..1631814 (+) 993 WP_014704192.1 response regulator -
  Q7C_RS07805 (Q7C_1625) - 1632005..1632763 (+) 759 WP_187287363.1 DUF2927 domain-containing protein -
  Q7C_RS07810 (Q7C_1626) - 1632819..1633664 (-) 846 WP_014704194.1 OmpA family protein -
  Q7C_RS07815 (Q7C_1627) pilU 1633826..1634971 (-) 1146 WP_014704195.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q7C_RS07820 (Q7C_1628) pilT 1634984..1636021 (-) 1038 WP_014704196.1 type IV pilus twitching motility protein PilT Machinery gene
  Q7C_RS07825 (Q7C_1629) - 1636104..1636799 (+) 696 WP_014704197.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q7C_RS07830 (Q7C_1630) proC 1636840..1637676 (+) 837 WP_014704198.1 pyrroline-5-carboxylate reductase -
  Q7C_RS07835 (Q7C_1631) - 1637666..1638244 (+) 579 WP_014704199.1 YggT family protein -
  Q7C_RS07840 (Q7C_1632) metX 1638300..1639448 (+) 1149 WP_014704200.1 homoserine O-succinyltransferase MetX -
  Q7C_RS07845 (Q7C_1633) metW 1639448..1640050 (+) 603 WP_014704201.1 methionine biosynthesis protein MetW -
  Q7C_RS07850 (Q7C_1634) pyrE 1640047..1640688 (+) 642 WP_014704202.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38468.19 Da        Isoelectric Point: 6.8893

>NTDB_id=51033 Q7C_RS07820 WP_014704196.1 1634984..1636021(-) (pilT) [Methylophaga frappieri]
MDITELLTFSAKNNASDLHISSGEPPMIRVDGDVKRINLPPMEHKDVHSMIYDIMNDKQRKDFEEFLEADFSFEVPNLAR
FRVNAFNQNRGAAAVFRTIPSVVLTLEQLGAPDIFKQIADNHRGIVLVTGPTGSGKSTTLAAMINYRNEKDNEHILTIED
PIEFVHKSKKCLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKAMVRSMLSESLRAVISQTLLKKIGGGRIAAHEIMIGTPAIRNLIREDKVPQMYSAIQTGGAIGMQTLDQCL
QDLIRRQQITKGEALPRAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=51033 Q7C_RS07820 WP_014704196.1 1634984..1636021(-) (pilT) [Methylophaga frappieri]
ATGGATATTACTGAACTGCTTACCTTCAGTGCAAAAAACAACGCCTCGGATTTGCACATATCCTCGGGTGAGCCGCCTAT
GATTCGTGTCGATGGTGATGTTAAGCGGATTAATCTACCGCCAATGGAACACAAAGATGTTCACAGCATGATTTACGACA
TCATGAATGACAAACAGCGCAAAGACTTTGAGGAGTTTCTTGAAGCCGACTTTTCATTTGAAGTGCCAAATCTGGCGCGT
TTTCGGGTCAATGCATTCAATCAAAATCGTGGTGCGGCGGCTGTTTTTCGGACCATTCCTTCGGTCGTTCTCACTTTAGA
GCAACTTGGGGCGCCAGATATCTTTAAGCAAATTGCCGATAATCATCGCGGCATCGTTTTGGTGACAGGACCGACCGGTA
GTGGTAAGTCCACCACGCTGGCGGCAATGATTAATTATCGTAATGAAAAAGATAATGAGCATATTCTGACGATTGAAGAT
CCGATAGAGTTTGTGCACAAAAGTAAAAAATGCTTGATCAACCAGCGTGAAGTACACCGCGACACCCATGGCTTCAGTGA
AGCCTTGCGCAGTGCATTACGGGAAGATCCTGACATTATTTTGGTGGGTGAGCTACGGGATTTGGAAACCATCAGGCTGG
CTTTGACTGCTGCGGAAACAGGTCACTTGGTGTTTGGAACGTTGCATACCTCTTCAGCGGCGAAAACGATTGACAGGATT
ATTGACGTTTTCCCAGCAGCGGAAAAAGCCATGGTGCGGTCAATGTTATCAGAATCGTTACGGGCCGTTATTTCGCAGAC
ATTGTTGAAAAAAATAGGCGGTGGTCGTATTGCTGCCCATGAAATTATGATTGGGACGCCAGCAATTCGTAACCTGATTC
GCGAAGACAAAGTCCCGCAAATGTACTCAGCAATCCAAACCGGTGGCGCGATCGGTATGCAGACTCTGGATCAATGCTTA
CAAGATCTGATTCGTCGTCAGCAAATTACCAAAGGCGAGGCATTGCCTCGTGCGGTTAATAAAGACTTATTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1YIN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.713

98.841

0.768
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.62

94.493

0.696
  pilT Vibrio cholerae strain A1552

73.62

94.493

0.696
  pilT Neisseria meningitidis 8013

69.096

99.42

0.687
  pilT Neisseria gonorrhoeae MS11

68.805

99.42

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Pseudomonas stutzeri DSM 10701

41.593

98.261

0.409
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397

Multiple sequence alignment