Detailed information NTG0051032

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7C_RS07815
• Genome accession: NC_017856
• Coordinates: 1633826..1634971 (-)
• Length: 381 a.a.
• NCBI ID: WP_014704195.1
• Uniprot ID: I1YIN2
• Organism: Methylophaga frappieri
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1628826..1639971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7C_RS07800 (Q7C_1624)	-	1630822..1631814 (+)	993	WP_014704192.1	response regulator	-
Q7C_RS07805 (Q7C_1625)	-	1632005..1632763 (+)	759	WP_187287363.1	DUF2927 domain-containing protein	-
Q7C_RS07810 (Q7C_1626)	-	1632819..1633664 (-)	846	WP_014704194.1	OmpA family protein	-
Q7C_RS07815 (Q7C_1627)	pilU	1633826..1634971 (-)	1146	WP_014704195.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7C_RS07820 (Q7C_1628)	pilT	1634984..1636021 (-)	1038	WP_014704196.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7C_RS07825 (Q7C_1629)	-	1636104..1636799 (+)	696	WP_014704197.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7C_RS07830 (Q7C_1630)	proC	1636840..1637676 (+)	837	WP_014704198.1	pyrroline-5-carboxylate reductase	-
Q7C_RS07835 (Q7C_1631)	-	1637666..1638244 (+)	579	WP_014704199.1	YggT family protein	-
Q7C_RS07840 (Q7C_1632)	metX	1638300..1639448 (+)	1149	WP_014704200.1	homoserine O-succinyltransferase MetX	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42203.40 Da
• Isoelectric Point: 5.3413

>NTDB_id=51032 Q7C_RS07815 WP_014704195.1 1633826..1634971(-) (pilU) [Methylophaga frappieri]
MEIVTILKAAVKHEASDIFITAERPPTLKVNGRMATLSQEPLTDEQARNLVLSTMNDEQKRTFERDKECNYAIDAKGAGR
FRVSALYQRNRVGMVMRLVKEDIPTIEQLNVPEIIKDLSMTKRGLVIFVGATGSGKSTTLAAMIGYRNQNSTGHILTIED
PIEFSHKHAGCVVTQREVGVDTDSYAIALKNSLRQAPDVIMIGEIRSRDTMDYGIAFAETGHLCLSTLHANNANQALDRI
INFFPEDRHQQLFMDMSLNLKAVVAQRLIPKKDGIGLCVAVEVMVNTPLIGDLIEKGKVSEIKDVMKRSRELGMQTFDQA
VYDLYKAGQISYEDALKNADSTNEVRLMIKLGAETGELPESLADDGGLALEPENEGFMGRL

Nucleotide

• Length: 1146 bp

>NTDB_id=51032 Q7C_RS07815 WP_014704195.1 1633826..1634971(-) (pilU) [Methylophaga frappieri]
ATGGAAATTGTCACCATTCTCAAGGCAGCGGTAAAACATGAAGCGTCGGATATATTCATTACGGCAGAGCGGCCGCCGAC
ATTGAAAGTCAATGGGCGTATGGCAACATTATCGCAAGAACCGTTGACAGATGAGCAGGCGCGTAATCTGGTGCTCAGCA
CCATGAATGATGAACAAAAAAGAACCTTTGAGCGTGATAAAGAATGTAATTACGCCATTGATGCCAAAGGGGCTGGACGT
TTTCGGGTGAGTGCGCTTTATCAGCGTAACCGGGTGGGAATGGTCATGCGCTTGGTCAAAGAGGACATCCCCACCATCGA
ACAATTGAATGTTCCGGAAATTATCAAAGATCTCTCTATGACCAAACGCGGGTTGGTTATTTTTGTCGGGGCAACCGGTA
GCGGTAAATCTACAACGCTGGCAGCCATGATTGGCTACCGTAATCAAAACAGCACCGGGCATATTTTAACTATCGAAGAT
CCAATCGAGTTCAGTCACAAACATGCTGGCTGTGTGGTCACTCAGCGTGAAGTCGGCGTCGATACCGATTCCTATGCCAT
TGCTCTGAAAAACTCTCTACGGCAGGCACCGGATGTCATCATGATTGGCGAGATTCGTTCTCGCGATACGATGGATTACG
GTATTGCCTTTGCTGAGACGGGGCATTTATGTTTATCGACATTACACGCGAACAATGCCAACCAGGCCTTGGACAGGATC
ATTAACTTTTTCCCTGAAGATCGTCATCAGCAACTGTTTATGGATATGTCGCTTAATTTAAAAGCAGTAGTAGCACAGCG
ACTCATTCCTAAAAAAGATGGCATCGGGCTCTGTGTCGCCGTTGAGGTCATGGTAAATACGCCACTGATTGGGGACTTGA
TTGAAAAAGGCAAAGTCTCTGAAATTAAAGATGTCATGAAGCGTTCTCGAGAACTAGGCATGCAGACCTTTGACCAGGCT
GTTTATGACTTATATAAAGCAGGGCAGATTTCTTATGAGGATGCTTTGAAAAATGCCGACTCAACAAATGAAGTCAGGCT
AATGATTAAACTGGGTGCTGAGACTGGTGAACTGCCGGAATCACTGGCTGATGATGGTGGTCTCGCATTGGAACCTGAAA
ACGAAGGTTTTATGGGACGCCTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-271)

3D structure

• AlphaFold DB: I1YIN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.366	97.638	0.609
pilU	Acinetobacter baylyi ADP1	57.821	93.963	0.543
pilU	Vibrio cholerae strain A1552	53.429	91.864	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	88.976	0.373