Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I4484_RS19485 Genome accession   NZ_CP065435
Coordinates   4165669..4166703 (-) Length   344 a.a.
NCBI ID   WP_197448814.1    Uniprot ID   -
Organism   Halomonas sp. SS10-MC5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4160669..4171703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I4484_RS19470 (I4484_19470) tyrS 4162032..4163231 (-) 1200 WP_197448811.1 tyrosine--tRNA ligase -
  I4484_RS19475 (I4484_19475) - 4163349..4164479 (+) 1131 WP_197448812.1 anhydro-N-acetylmuramic acid kinase -
  I4484_RS19480 (I4484_19480) pilU 4164495..4165637 (-) 1143 WP_197448813.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I4484_RS19485 (I4484_19485) pilT 4165669..4166703 (-) 1035 WP_197448814.1 type IV pilus twitching motility protein PilT Machinery gene
  I4484_RS19490 (I4484_19490) - 4166782..4167492 (+) 711 WP_197448815.1 YggS family pyridoxal phosphate-dependent enzyme -
  I4484_RS19495 (I4484_19495) proC 4167552..4168376 (+) 825 WP_197448816.1 pyrroline-5-carboxylate reductase -
  I4484_RS19500 (I4484_19500) - 4168435..4169025 (+) 591 WP_197448817.1 YggT family protein -
  I4484_RS19505 (I4484_19505) - 4169072..4170229 (+) 1158 WP_197451124.1 homoserine O-acetyltransferase -
  I4484_RS19510 (I4484_19510) metW 4170229..4170831 (+) 603 WP_197451125.1 methionine biosynthesis protein MetW -
  I4484_RS19515 (I4484_19515) - 4170874..4171335 (+) 462 WP_197448818.1 DUF4426 domain-containing protein -
  I4484_RS19520 (I4484_19520) - 4171344..4171661 (+) 318 WP_197448819.1 Rieske 2Fe-2S domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38080.58 Da        Isoelectric Point: 6.5965

>NTDB_id=509652 I4484_RS19485 WP_197448814.1 4165669..4166703(-) (pilT) [Halomonas sp. SS10-MC5]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMGDRQRRDYEEFFETDFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPSEVLSMEDLGLGEVFRRLALLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYDHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAQALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVISQTLLKRRSGGRVAAHEILVANAAVRNLIREDKVAQIYSAIQTGGNLGMQTLDAAL
AKLVAENVINRDEAQARAKSVLTG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=509652 I4484_RS19485 WP_197448814.1 4165669..4166703(-) (pilT) [Halomonas sp. SS10-MC5]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAGAACGCCTCCGACCTGCATCTGTCGGCCGGTCTGCCGCCGAT
GATTCGGGTCGATGGCGACATTCGTCGCCTCAACGTGCCGGCCATGGAAGACCGCGAAGTCCGCAAGCTGATCTACGACA
TCATGGGCGACCGCCAGCGCCGTGACTACGAAGAGTTCTTCGAGACCGACTTCTCCTTCGAGGTCCCCGGGGTGGCTCGC
TTCCGCGTCAACGCCTTCAACCAGGCCCGCGGTGCCGGTGCGGTGTTTCGCACCATTCCCAGCGAGGTGCTGAGCATGGA
GGACCTGGGTCTGGGCGAGGTCTTCCGCCGCCTGGCGCTGCTGCCGCGCGGGCTGGTGCTGGTCACCGGCCCCACCGGGT
CGGGCAAGAGCACGACCCTGGCCGCGATGATCGACTATATCAACGATCACCGCTACGACCATATCCTCACCATCGAGGAC
CCGGTGGAATTCGTCCACCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGTGACACGCACGGCTTCGCCCA
GGCGCTGCGCAGCGCACTGCGCGAAGACCCCGACGTGATCCTGGTCGGCGAGCTGCGCGACCTCGAGACCATCCGCCTGG
CGCTCACCGCCGCCGAGACCGGCCACCTGGTGTTCGGTACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGCGAGGAGAAGGCCATGGTGCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTCAAGCGCCGCAGCGGGGGGCGGGTGGCGGCCCACGAAATCCTCGTCGCCAACGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCCATCCAGACCGGCGGCAACCTGGGGATGCAGACCCTCGACGCCGCCCTG
GCCAAGCTGGTGGCCGAAAACGTCATCAATCGCGACGAGGCGCAGGCGCGTGCCAAGAGCGTGCTGACTGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.646

98.547

0.785
  pilT Pseudomonas aeruginosa PAK

79.351

98.547

0.782
  pilT Acinetobacter baumannii D1279779

78.171

98.547

0.77
  pilT Acinetobacter baumannii strain A118

78.171

98.547

0.77
  pilT Acinetobacter nosocomialis M2

77.876

98.547

0.767
  pilT Acinetobacter baylyi ADP1

77.353

98.837

0.765
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.155

99.128

0.666
  pilT Neisseria gonorrhoeae MS11

66.862

99.128

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.572

98.547

0.538
  pilU Pseudomonas stutzeri DSM 10701

41.349

99.128

0.41
  pilU Vibrio cholerae strain A1552

41.056

99.128

0.407
  pilU Acinetobacter baylyi ADP1

40.351

99.419

0.401