Detailed information NTG0509651

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I4484_RS19480
• Genome accession: NZ_CP065435
• Coordinates: 4164495..4165637 (-)
• Length: 380 a.a.
• NCBI ID: WP_197448813.1
• Uniprot ID: -
• Organism: Halomonas sp. SS10-MC5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4159495..4170637

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I4484_RS19470 (I4484_19470)	tyrS	4162032..4163231 (-)	1200	WP_197448811.1	tyrosine--tRNA ligase	-
I4484_RS19475 (I4484_19475)	-	4163349..4164479 (+)	1131	WP_197448812.1	anhydro-N-acetylmuramic acid kinase	-
I4484_RS19480 (I4484_19480)	pilU	4164495..4165637 (-)	1143	WP_197448813.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I4484_RS19485 (I4484_19485)	pilT	4165669..4166703 (-)	1035	WP_197448814.1	type IV pilus twitching motility protein PilT	Machinery gene
I4484_RS19490 (I4484_19490)	-	4166782..4167492 (+)	711	WP_197448815.1	YggS family pyridoxal phosphate-dependent enzyme	-
I4484_RS19495 (I4484_19495)	proC	4167552..4168376 (+)	825	WP_197448816.1	pyrroline-5-carboxylate reductase	-
I4484_RS19500 (I4484_19500)	-	4168435..4169025 (+)	591	WP_197448817.1	YggT family protein	-
I4484_RS19505 (I4484_19505)	-	4169072..4170229 (+)	1158	WP_197451124.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42570.61 Da
• Isoelectric Point: 6.0988

>NTDB_id=509651 I4484_RS19480 WP_197448813.1 4164495..4165637(-) (pilU) [Halomonas sp. SS10-MC5]
MKAQEWLEQLLDIMARQEASDLLISVQAPPTIKTAGKLTALGDQRLSVEQVRELVNTAVPEGLRERFAQEREANFALSLS
GRGRFRVSAFQQRNQWAMVIRRIAYEIPHLEELSLPPQLGELANLKRGLVFVVGGTGTGKSTTLASMIQQRNETVGGHII
SVEDPIEYVHPHKKAIINQREVGIDTESFEVALKNTLRQAPDVVLIGEVRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLHAIVAQQLLPTRDGGRAPAIEILLRTPLIADLIRKGDVGEIKNVMARSRDQGMQTF
DQALFELYKAGKISEEVAMVHADSANELRMMIKYGDEKSDVLAKAQDGAQRFSLRDDDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=509651 I4484_RS19480 WP_197448813.1 4164495..4165637(-) (pilU) [Halomonas sp. SS10-MC5]
ATGAAAGCACAGGAATGGTTGGAGCAGCTGCTCGACATCATGGCCCGCCAGGAGGCCTCGGACCTGCTGATCTCGGTGCA
GGCGCCGCCCACCATCAAGACCGCCGGCAAGCTCACCGCGCTGGGCGACCAGCGGCTCTCGGTGGAACAGGTGCGCGAGC
TGGTCAACACCGCGGTGCCTGAAGGGCTCAGGGAGCGCTTTGCGCAGGAGCGCGAAGCCAACTTCGCGCTCAGCCTCAGC
GGGCGGGGGCGCTTTCGTGTCAGCGCCTTCCAGCAGCGCAACCAGTGGGCCATGGTCATCCGTCGCATCGCCTACGAGAT
CCCCCATCTCGAAGAGCTCTCGCTGCCGCCGCAGCTGGGCGAGCTGGCCAATCTCAAGCGTGGGCTGGTGTTCGTGGTGG
GCGGCACCGGTACCGGCAAGTCGACCACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCGTCGGCGGTCACATCATC
AGCGTCGAGGACCCCATCGAGTACGTGCACCCGCACAAGAAGGCGATCATCAACCAGCGCGAGGTGGGCATCGATACCGA
GTCCTTCGAGGTGGCGTTGAAGAACACCTTGCGCCAGGCACCGGACGTGGTGCTGATCGGCGAGGTGCGCACCCGCGAAA
CCATGGAGCACGCCCTGACCTTCGCCGAGACGGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCG
CTCGATCGCATCATTCACTTCTTCCCCCACGAACGCCACGAGCAGATCTGGCTCGACCTGTCGCTCAACCTGCATGCCAT
CGTCGCGCAGCAGCTGCTGCCCACCCGCGATGGCGGGCGCGCCCCGGCTATCGAGATTCTGCTGCGCACGCCGCTGATCG
CCGACCTGATTCGCAAGGGCGACGTGGGCGAGATCAAGAACGTCATGGCACGTTCGCGCGACCAGGGCATGCAGACCTTC
GACCAGGCGCTGTTCGAGCTCTACAAGGCCGGCAAGATCAGCGAGGAAGTGGCCATGGTGCACGCCGACTCGGCCAACGA
GCTGCGCATGATGATCAAGTACGGCGACGAGAAGAGCGACGTGCTGGCCAAGGCGCAGGACGGCGCGCAGCGGTTTTCGC
TGCGCGACGATGATGACTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (16-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.517	98.158	0.584
pilU	Vibrio cholerae strain A1552	58.621	91.579	0.537
pilU	Acinetobacter baylyi ADP1	54.918	96.316	0.529
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.679	85.263	0.389
pilT	Neisseria meningitidis 8013	42.042	87.632	0.368
pilT	Neisseria gonorrhoeae MS11	41.742	87.632	0.366
pilT	Legionella pneumophila strain ERS1305867	41.566	87.368	0.363
pilT	Legionella pneumophila strain Lp02	41.566	87.368	0.363