Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IT895_RS01670 Genome accession   NZ_CP065230
Coordinates   331288..332331 (+) Length   347 a.a.
NCBI ID   WP_085920178.1    Uniprot ID   -
Organism   Halomonas sp. A40-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326288..337331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IT895_RS01640 (IT895_01635) sfsA 326435..327226 (+) 792 WP_199284518.1 DNA/RNA nuclease SfsA -
  IT895_RS01645 (IT895_01640) metW 327173..327766 (-) 594 WP_066316286.1 methionine biosynthesis protein MetW -
  IT895_RS01650 (IT895_01645) - 327766..328962 (-) 1197 WP_199284519.1 homoserine O-acetyltransferase -
  IT895_RS01655 (IT895_01650) - 329016..329609 (-) 594 WP_085920175.1 YggT family protein -
  IT895_RS01660 (IT895_01655) proC 329639..330463 (-) 825 WP_085920176.1 pyrroline-5-carboxylate reductase -
  IT895_RS01665 (IT895_01660) - 330550..331254 (-) 705 WP_199284520.1 YggS family pyridoxal phosphate-dependent enzyme -
  IT895_RS01670 (IT895_01665) pilT 331288..332331 (+) 1044 WP_085920178.1 type IV pilus twitching motility protein PilT Machinery gene
  IT895_RS01675 (IT895_01670) pilU 332335..333501 (+) 1167 WP_085920179.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IT895_RS01680 (IT895_01675) - 333512..334642 (-) 1131 WP_199284521.1 anhydro-N-acetylmuramic acid kinase -
  IT895_RS01685 (IT895_01680) tyrS 334758..335957 (+) 1200 WP_199284522.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38096.54 Da        Isoelectric Point: 6.2344

>NTDB_id=508243 IT895_RS01670 WP_085920178.1 331288..332331(+) (pilT) [Halomonas sp. A40-4]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNSEVRRLIHDIMNDRQRRDYEEHLEVDFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNQVLSMKTLGLGEVFERLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHASKRCLINQREVHRDTHGFAEALRSALREDPDVILVGELRDQETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVSQALLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIFSAIQTGGNLGMQTLDAAL
MQLVNDGHVGMEDAQALAKGTLSSREA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=508243 IT895_RS01670 WP_085920178.1 331288..332331(+) (pilT) [Halomonas sp. A40-4]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCTTCAGACCTCCACTTATCCGCGGGCTTGCCGCCCAT
GATACGGGTTGATGGCGATATCCGTCGGCTGAATGTGCCCGCCATGGATAACAGTGAGGTCCGTCGGTTGATTCACGACA
TCATGAACGATCGCCAGCGTCGCGACTACGAAGAACACCTTGAAGTCGACTTTTCTTTCGAAGTGCCGGGCGTGGCGCGT
TTTCGGGTCAATGCCTTTAATCAGGCACGCGGTGCGGGAGCGGTATTTCGCACCATTCCCAACCAGGTGCTATCGATGAA
GACCCTGGGGCTGGGGGAAGTGTTCGAGCGCCTTTCAATGCTGCCGCGCGGTCTGGTGTTGGTGACCGGGCCAACCGGTT
CGGGAAAAAGTACCACACTCGCGGCCATGATCGATTACATCAACGATCATCGCTTTGAACACATTTTAACCATCGAAGAC
CCGGTTGAGTTTGTGCATGCCAGCAAGCGTTGCCTGATTAATCAGCGGGAAGTCCATCGTGACACGCATGGCTTTGCGGA
GGCATTGCGCAGTGCGCTGCGCGAAGACCCGGATGTGATTCTGGTGGGAGAGCTGCGCGATCAGGAAACCATCCGCCTGG
CGTTAACAGCGGCAGAGACCGGACACCTGGTGCTCGGGACGCTGCATACCACCTCAGCGGCCAAGACCATTGACCGGATT
ATTGATGTCTTCCCCGGCGAAGAGAAGGCCATGGTGCGTTCGATGCTGTCGGAGTCGCTACAGGCCGTGGTATCCCAGGC
GCTGTTGAAACGCCAGGGGGGCGGCCGTGTGGCGGCCCATGAAATTCTGATCGCGACCTCGGCGGTGCGCAATCTGATTC
GTGAAGACAAAGTGGCGCAGATATTCTCGGCGATTCAAACCGGCGGCAATCTGGGTATGCAAACGTTGGACGCCGCACTG
ATGCAACTGGTCAACGACGGTCATGTCGGCATGGAAGATGCCCAGGCGCTGGCCAAGGGCACCCTCTCTTCGCGGGAGGC
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.581

97.695

0.758
  pilT Pseudomonas stutzeri DSM 10701

76.991

97.695

0.752
  pilT Acinetobacter baylyi ADP1

75.811

97.695

0.741
  pilT Acinetobacter baumannii strain A118

75.516

97.695

0.738
  pilT Acinetobacter nosocomialis M2

75.516

97.695

0.738
  pilT Acinetobacter baumannii D1279779

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

69.527

97.406

0.677
  pilT Legionella pneumophila strain ERS1305867

69.527

97.406

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.94

96.83

0.677
  pilT Vibrio cholerae strain A1552

69.94

96.83

0.677
  pilT Neisseria meningitidis 8013

66.279

99.135

0.657
  pilT Neisseria gonorrhoeae MS11

65.988

99.135

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.938

93.372

0.513
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395