Detailed information NTG0508244

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IT895_RS01675
• Genome accession: NZ_CP065230
• Coordinates: 332335..333501 (+)
• Length: 388 a.a.
• NCBI ID: WP_085920179.1
• Uniprot ID: A0A7T0WED4
• Organism: Halomonas sp. A40-4
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 327335..338501

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IT895_RS01650 (IT895_01645)	-	327766..328962 (-)	1197	WP_199284519.1	homoserine O-acetyltransferase	-
IT895_RS01655 (IT895_01650)	-	329016..329609 (-)	594	WP_085920175.1	YggT family protein	-
IT895_RS01660 (IT895_01655)	proC	329639..330463 (-)	825	WP_085920176.1	pyrroline-5-carboxylate reductase	-
IT895_RS01665 (IT895_01660)	-	330550..331254 (-)	705	WP_199284520.1	YggS family pyridoxal phosphate-dependent enzyme	-
IT895_RS01670 (IT895_01665)	pilT	331288..332331 (+)	1044	WP_085920178.1	type IV pilus twitching motility protein PilT	Machinery gene
IT895_RS01675 (IT895_01670)	pilU	332335..333501 (+)	1167	WP_085920179.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IT895_RS01680 (IT895_01675)	-	333512..334642 (-)	1131	WP_199284521.1	anhydro-N-acetylmuramic acid kinase	-
IT895_RS01685 (IT895_01680)	tyrS	334758..335957 (+)	1200	WP_199284522.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43027.00 Da
• Isoelectric Point: 6.5516

>NTDB_id=508244 IT895_RS01675 WP_085920179.1 332335..333501(+) (pilU) [Halomonas sp. A40-4]
MTNNDASTLTPGQWLHQLLDIMVTQQASDLLISTGAPPSLKMPSGLVPLGQRPLSEHQVSELVTHTVPEGLRERFSQQRE
ANFALGFEDKGRFRVSAFQQRNQPAMVIRRIEQHIPTLETLGVPTVLASLAHAKRGLVLVVGGTGTGKSTTLASMVQERN
ETIGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMEHALTFAETGHLCLATL
HANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPRLNGGRCAAVEVMLPSSRIADLIRKGRVAEIKAAMAAS
RDDGMQTFDQALFDLCQASQISHEVALAHADSANDLRLMLKLQGEATSRSLDERAPSHWDLRDRDDAW

Nucleotide

• Length: 1167 bp

>NTDB_id=508244 IT895_RS01675 WP_085920179.1 332335..333501(+) (pilU) [Halomonas sp. A40-4]
ATGACGAACAACGACGCCTCAACGCTGACGCCTGGCCAGTGGTTGCATCAGCTGTTGGACATCATGGTGACGCAGCAAGC
CTCAGACCTACTCATTTCAACGGGGGCACCACCCAGCCTCAAGATGCCCAGCGGGCTGGTTCCTCTTGGCCAACGGCCCT
TGAGTGAACATCAGGTAAGTGAGCTGGTAACGCACACCGTGCCTGAAGGGCTGCGCGAGCGTTTTAGCCAACAGCGCGAG
GCGAATTTTGCGCTGGGTTTTGAAGACAAAGGGCGCTTTCGCGTTAGCGCCTTCCAGCAGCGAAATCAGCCCGCCATGGT
GATTCGCCGTATTGAGCAGCACATTCCCACCCTGGAAACCCTGGGTGTGCCCACGGTGCTTGCCTCGCTCGCCCATGCCA
AACGGGGGCTGGTATTGGTGGTAGGGGGCACGGGGACCGGGAAATCCACCACCCTGGCGTCGATGGTGCAGGAGCGCAAT
GAAACGATCGGTGGGCACATCATCAGCATTGAAGATCCGATCGAGTACCTGCACCCGCACAAGCGCGCCATCGTTAACCA
GCGTGAAGTGGGGATTGATACCGAGTCATTTGAAGTAGCGCTCAAGAACACCCTGCGCCAAGCCCCTGATGTGATCTTGA
TCGGTGAGATACGCAGCCGTGAAACCATGGAACATGCGTTGACGTTCGCTGAAACAGGGCATCTCTGTCTGGCCACCTTG
CACGCCAACAACGCCAATCAGGCGTTGGAGCGTATCATTCACTTCTTTCCTCATGAGCGTCATGAGCAGATATGGATGGA
CCTCTCCCTCAATCTCCGCGCGGTGGTGGCTCAACAGCTGTTGCCCAGGCTGAACGGTGGTCGTTGTGCGGCGGTTGAAG
TCATGCTGCCGTCGTCACGGATTGCCGACCTGATTCGTAAAGGCAGGGTCGCCGAGATTAAAGCCGCCATGGCGGCGTCG
CGTGATGACGGCATGCAGACGTTTGATCAAGCACTCTTCGATCTTTGCCAGGCATCACAGATTAGTCATGAGGTAGCGCT
TGCCCACGCGGACTCCGCCAATGACTTGCGACTCATGCTGAAGCTGCAAGGGGAGGCGACGTCGCGTTCGCTGGATGAGC
GTGCTCCAAGTCATTGGGATCTGCGCGACCGCGACGATGCATGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-282)

3D structure

• AlphaFold DB: A0A7T0WED4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	61.782	89.691	0.554
pilU	Pseudomonas stutzeri DSM 10701	59.777	92.268	0.552
pilU	Acinetobacter baylyi ADP1	56.63	93.299	0.528
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.44	88.402	0.384