Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IWH25_RS02385 Genome accession   NZ_CP064781
Coordinates   498216..499259 (-) Length   347 a.a.
NCBI ID   WP_203387769.1    Uniprot ID   A0A974SPT2
Organism   Azospira restricta strain DSM 18626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 493216..504259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS02360 (IWH25_02360) - 493279..494316 (-) 1038 WP_203387764.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  IWH25_RS02365 (IWH25_02365) - 494382..495281 (+) 900 WP_203387765.1 SDR family oxidoreductase -
  IWH25_RS02370 (IWH25_02370) - 495269..495685 (+) 417 WP_203387766.1 CopD family protein -
  IWH25_RS02375 (IWH25_02375) - 495687..496835 (+) 1149 WP_203387767.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  IWH25_RS02380 (IWH25_02380) pilU 497049..498185 (-) 1137 WP_203387768.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IWH25_RS02385 (IWH25_02385) pilT 498216..499259 (-) 1044 WP_203387769.1 type IV pilus twitching motility protein PilT Machinery gene
  IWH25_RS02390 (IWH25_02390) - 499330..500013 (+) 684 WP_203387770.1 YggS family pyridoxal phosphate-dependent enzyme -
  IWH25_RS02395 (IWH25_02395) proC 500016..500828 (+) 813 WP_203387771.1 pyrroline-5-carboxylate reductase -
  IWH25_RS02400 (IWH25_02400) - 500848..501384 (+) 537 WP_203387772.1 YggT family protein -
  IWH25_RS02405 (IWH25_02405) - 501390..501683 (+) 294 WP_203389124.1 DUF167 domain-containing protein -
  IWH25_RS02410 (IWH25_02410) - 501741..502637 (-) 897 WP_203387773.1 ethylbenzene dehydrogenase-related protein -
  IWH25_RS02415 (IWH25_02415) - 502752..504008 (-) 1257 WP_203387774.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38329.08 Da        Isoelectric Point: 6.9271

>NTDB_id=504690 IWH25_RS02385 WP_203387769.1 498216..499259(-) (pilT) [Azospira restricta strain DSM 18626]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMEHKDVHAMVYDIMNDGQRKIYEETLECDFSFEVPNLAR
FRVNAFNQHRGAGAVFRTIPSKVLTLEDLNCPKIFKDISEFPRGVVLVTGPTGSGKSTTLAAMVNHVNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGQQFGMQTLDQC
LIDLVRRNVVSGNEARTKAANKDAIPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=504690 IWH25_RS02385 WP_203387769.1 498216..499259(-) (pilT) [Azospira restricta strain DSM 18626]
ATGGATATCACCGAACTGCTCGCCTTCGGCGTCAAGAACAAGGCTTCCGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCCATGGCGACGTGCGCCGCATCAACCTGCCGGCGATGGAGCACAAGGACGTGCATGCGATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGATCTACGAGGAGACGCTGGAGTGCGACTTTTCGTTCGAGGTGCCGAACCTCGCCCGC
TTCCGCGTCAACGCGTTCAACCAGCACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCGAAGGTGCTGACGCTGGA
AGATCTCAACTGCCCGAAGATCTTCAAGGACATCTCCGAATTCCCGCGCGGCGTCGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCGACGACGCTGGCGGCGATGGTCAACCACGTCAACGAGAACGAGTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGCATACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAAATGCGCGACCTGGAGACGATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCGAAGACCATCGACCGCATC
ATCGACGTCTTCCCGGCGGCGGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGTTGAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCGCACGAGATCATGATCGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAGGCGAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGC
CTGATCGACCTGGTCCGGCGCAACGTCGTCTCCGGCAACGAGGCGCGCACCAAGGCGGCGAACAAGGATGCGATCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.29

99.424

0.798
  pilT Acinetobacter baumannii D1279779

80.29

99.424

0.798
  pilT Acinetobacter baumannii strain A118

80.29

99.424

0.798
  pilT Acinetobacter baylyi ADP1

76.812

99.424

0.764
  pilT Pseudomonas aeruginosa PAK

77.876

97.695

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.581

97.695

0.758
  pilT Legionella pneumophila strain Lp02

73.392

98.559

0.723
  pilT Legionella pneumophila strain ERS1305867

73.392

98.559

0.723
  pilT Neisseria meningitidis 8013

71.594

99.424

0.712
  pilT Neisseria gonorrhoeae MS11

71.304

99.424

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.822

97.406

0.68
  pilT Vibrio cholerae strain A1552

69.822

97.406

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.212

97.695

0.51
  pilU Acinetobacter baylyi ADP1

41.964

96.83

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403