Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IWH25_RS02380 Genome accession   NZ_CP064781
Coordinates   497049..498185 (-) Length   378 a.a.
NCBI ID   WP_203387768.1    Uniprot ID   A0A974SPT6
Organism   Azospira restricta strain DSM 18626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492049..503185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS02355 (IWH25_02355) - 492066..493235 (-) 1170 WP_203387763.1 EAL and HDOD domain-containing protein -
  IWH25_RS02360 (IWH25_02360) - 493279..494316 (-) 1038 WP_203387764.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  IWH25_RS02365 (IWH25_02365) - 494382..495281 (+) 900 WP_203387765.1 SDR family oxidoreductase -
  IWH25_RS02370 (IWH25_02370) - 495269..495685 (+) 417 WP_203387766.1 CopD family protein -
  IWH25_RS02375 (IWH25_02375) - 495687..496835 (+) 1149 WP_203387767.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  IWH25_RS02380 (IWH25_02380) pilU 497049..498185 (-) 1137 WP_203387768.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IWH25_RS02385 (IWH25_02385) pilT 498216..499259 (-) 1044 WP_203387769.1 type IV pilus twitching motility protein PilT Machinery gene
  IWH25_RS02390 (IWH25_02390) - 499330..500013 (+) 684 WP_203387770.1 YggS family pyridoxal phosphate-dependent enzyme -
  IWH25_RS02395 (IWH25_02395) proC 500016..500828 (+) 813 WP_203387771.1 pyrroline-5-carboxylate reductase -
  IWH25_RS02400 (IWH25_02400) - 500848..501384 (+) 537 WP_203387772.1 YggT family protein -
  IWH25_RS02405 (IWH25_02405) - 501390..501683 (+) 294 WP_203389124.1 DUF167 domain-containing protein -
  IWH25_RS02410 (IWH25_02410) - 501741..502637 (-) 897 WP_203387773.1 ethylbenzene dehydrogenase-related protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42146.51 Da        Isoelectric Point: 6.6619

>NTDB_id=504689 IWH25_RS02380 WP_203387768.1 497049..498185(-) (pilU) [Azospira restricta strain DSM 18626]
MERDQALKFMHDLLRLMVQKKGSDLFITAGFPPAIKIDGKITPVSNQTLSAQHTAELARSIMNDRQAADFEASKECNFAI
SPSGIGRFRVNALVQQGRVAVVCRTINMTIPNLDELGLPTVIKDIGMTKRGLIIFVGGTGTGKTTSLAAMVDYRNANSYG
HIITIEDPIEYVHEHKNCIVTQREVGIDTDTWEAALKNTLRQAPDVILMGEIRDRETMDYAVAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPKKDGKGRVAAVEVMLNSPLISDLIFKGEIHEIKEIMKKSRELG
MQTFDQALFDLYESGKITYEDALRNADSVNDLRLQIKLHGKESKDRDLTTGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=504689 IWH25_RS02380 WP_203387768.1 497049..498185(-) (pilU) [Azospira restricta strain DSM 18626]
ATGGAACGAGACCAGGCACTCAAATTCATGCACGACCTGCTGCGCCTGATGGTGCAGAAGAAAGGCTCGGACCTGTTCAT
CACCGCCGGCTTCCCGCCGGCGATCAAGATCGACGGCAAGATCACGCCGGTGTCGAACCAGACGCTGTCGGCGCAGCACA
CCGCCGAGCTGGCGCGGTCGATCATGAACGACCGCCAGGCCGCGGACTTCGAGGCGAGCAAGGAGTGCAACTTCGCCATC
TCGCCGTCGGGGATCGGCCGTTTCCGCGTGAACGCGCTGGTGCAGCAGGGGCGCGTCGCCGTCGTCTGTCGCACGATCAA
CATGACCATCCCGAACCTTGACGAGCTCGGCCTGCCGACCGTCATCAAGGACATCGGCATGACCAAGCGCGGGCTGATCA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTGGCGGCGATGGTCGACTACCGCAACGCCAACAGCTACGGC
CACATCATCACCATCGAGGATCCGATCGAGTACGTGCACGAGCACAAGAACTGCATCGTCACGCAGCGCGAGGTCGGCAT
CGATACCGACACCTGGGAGGCGGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGACTACGCGGTCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCATGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCAATGGTCTCGCAGCGCCTGCTGCCGAAGAAGGACGGCAAGGGCCGCGTCGCCGCGGTCGAGGTGATGCTCAATT
CGCCGCTGATCTCCGATCTGATCTTCAAGGGCGAGATCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAGTCGGGCAAGATCACCTACGAGGATGCGCTGAGGAACGCCGA
CTCGGTGAACGACCTGCGGCTGCAGATCAAGCTGCACGGCAAGGAATCGAAGGACCGCGACCTCACCACCGGCATCGGCC
ACCTCGACATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.084

94.709

0.616
  pilU Acinetobacter baylyi ADP1

60.446

94.974

0.574
  pilU Vibrio cholerae strain A1552

53.959

90.212

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.624

0.399
  pilT Acinetobacter baylyi ADP1

43.027

89.153

0.384
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Legionella pneumophila strain ERS1305867

43.202

87.566

0.378
  pilT Legionella pneumophila strain Lp02

43.202

87.566

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

87.302

0.362
  pilT Vibrio cholerae strain A1552

41.515

87.302

0.362