Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IVG45_RS14555 Genome accession   NZ_CP064653
Coordinates   3139873..3140910 (-) Length   345 a.a.
NCBI ID   WP_196434529.1    Uniprot ID   -
Organism   Methylomonas sp. LL1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3134873..3145910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IVG45_RS14525 (IVG45_14505) - 3135051..3135254 (+) 204 WP_196434523.1 cold-shock protein -
  IVG45_RS14530 (IVG45_14510) - 3135383..3136315 (+) 933 WP_196434524.1 hypothetical protein -
  IVG45_RS14535 (IVG45_14515) - 3136423..3136677 (+) 255 WP_196434525.1 hypothetical protein -
  IVG45_RS14540 (IVG45_14520) - 3137057..3137425 (-) 369 WP_196434526.1 hypothetical protein -
  IVG45_RS14545 (IVG45_14525) mltB 3137554..3138711 (-) 1158 WP_196434527.1 lytic murein transglycosylase B -
  IVG45_RS14550 (IVG45_14530) pilU 3138725..3139858 (-) 1134 WP_196434528.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IVG45_RS14555 (IVG45_14535) pilT 3139873..3140910 (-) 1038 WP_196434529.1 type IV pilus twitching motility protein PilT Machinery gene
  IVG45_RS14560 (IVG45_14540) - 3140964..3141185 (-) 222 WP_196434530.1 hypothetical protein -
  IVG45_RS14565 (IVG45_14545) - 3141188..3141529 (-) 342 WP_196434531.1 TraR/DksA family transcriptional regulator -
  IVG45_RS14570 (IVG45_14550) - 3141713..3142942 (+) 1230 WP_196434532.1 NADP-dependent isocitrate dehydrogenase -
  IVG45_RS14575 (IVG45_14555) - 3143037..3144092 (-) 1056 WP_196434533.1 fatty acid desaturase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38249.18 Da        Isoelectric Point: 6.8325

>NTDB_id=504048 IVG45_RS14555 WP_196434529.1 3139873..3140910(-) (pilT) [Methylomonas sp. LL1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALDHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELGAPKFFEEVCTKPRGLILVTGPTGSGKSTTLAAMINHINCNDYSHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
KELVDKGLITAKAAMVKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=504048 IVG45_RS14555 WP_196434529.1 3139873..3140910(-) (pilT) [Methylomonas sp. LL1]
ATGGACATCGCCGAATTATTGACCTTTTCCGTTAAAAACAAAGCTTCGGATTTACATCTTTCCGCCGGCCTACCACCGAT
GATCCGGGTGGACGGCGACATCCGCCGTATCAATATCCCGGCTTTGGATCATAAGGAAGTACATGCGCTGATTTACGACA
TCATGAACGATAAGCAGCGGCGAGATTACGAAGAGTTTCTGGAAACCGATTTTTCGTTTGCGTTGCCCGGCGTGGCTCGT
TTCAGGGTTAACGCTTTTAACCAGGATAGAGGCGCCGGTGCGGTATTTAGGACCATTCCATCCAAGGTGTTGACGCTGGA
AGAGTTGGGCGCGCCGAAATTTTTCGAGGAAGTCTGTACTAAACCGCGTGGCTTGATTCTGGTGACCGGCCCGACCGGCT
CCGGTAAATCCACCACGCTGGCGGCTATGATCAATCACATCAACTGCAATGATTACTCGCATATTTTGACCGTGGAAGAC
CCTATCGAGTTTGTGCATGAGAGCCAAAAATGTCTGATCAACCAGCGTGAGGTGCATCGCGACACCCTGGGCTTTAACGA
AGCCTTGCGTTCCGCGTTGCGGGAAGACCCCGACATCATTCTGGTGGGTGAGATGCGGGATTTGGAAACCATTCGTTTGG
CACTGACCGCCGCCGAAACCGGCCATTTGGTGTTCGGCACCTTGCATACCACCTCGGCCGCCAAAACCATAGACCGTATC
ATCGACGTATTTCCGGCGGCGGAAAAAGACATGATTCGTTCCATGCTGTCGGAATCGCTACAGGCGGTTATTTCGCAAAC
CCTGTTGAAGAAAGTTGGTGGTGGCCGGATCGCCGCGCATGAAATCATGGTCGGCACCGCCGCCATTCGTAACCTGATCC
GCGAAGCGAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGACGTAAGGAAGGCATGCAGACTCTCGATCAAAACCTC
AAGGAACTGGTTGACAAAGGTTTGATTACCGCCAAAGCCGCGATGGTGAAAGCCGTCAATAAAGACATGTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Pseudomonas aeruginosa PAK

81.361

97.971

0.797
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria gonorrhoeae MS11

66.472

99.42

0.661
  pilT Neisseria meningitidis 8013

66.472

99.42

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

39.595

100

0.397