Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IVG45_RS14550 Genome accession   NZ_CP064653
Coordinates   3138725..3139858 (-) Length   377 a.a.
NCBI ID   WP_196434528.1    Uniprot ID   A0A7T0NXA5
Organism   Methylomonas sp. LL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3133725..3144858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IVG45_RS14525 (IVG45_14505) - 3135051..3135254 (+) 204 WP_196434523.1 cold-shock protein -
  IVG45_RS14530 (IVG45_14510) - 3135383..3136315 (+) 933 WP_196434524.1 hypothetical protein -
  IVG45_RS14535 (IVG45_14515) - 3136423..3136677 (+) 255 WP_196434525.1 hypothetical protein -
  IVG45_RS14540 (IVG45_14520) - 3137057..3137425 (-) 369 WP_196434526.1 hypothetical protein -
  IVG45_RS14545 (IVG45_14525) mltB 3137554..3138711 (-) 1158 WP_196434527.1 lytic murein transglycosylase B -
  IVG45_RS14550 (IVG45_14530) pilU 3138725..3139858 (-) 1134 WP_196434528.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IVG45_RS14555 (IVG45_14535) pilT 3139873..3140910 (-) 1038 WP_196434529.1 type IV pilus twitching motility protein PilT Machinery gene
  IVG45_RS14560 (IVG45_14540) - 3140964..3141185 (-) 222 WP_196434530.1 hypothetical protein -
  IVG45_RS14565 (IVG45_14545) - 3141188..3141529 (-) 342 WP_196434531.1 TraR/DksA family transcriptional regulator -
  IVG45_RS14570 (IVG45_14550) - 3141713..3142942 (+) 1230 WP_196434532.1 NADP-dependent isocitrate dehydrogenase -
  IVG45_RS14575 (IVG45_14555) - 3143037..3144092 (-) 1056 WP_196434533.1 fatty acid desaturase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42355.74 Da        Isoelectric Point: 6.5037

>NTDB_id=504047 IVG45_RS14550 WP_196434528.1 3138725..3139858(-) (pilU) [Methylomonas sp. LL1]
MDFKALLALMVEKKASDLFITAGKPPCMKIDGKVVEISKNILTADQALKIVLSIMEQRQKDEFEHTKECQFAMGVEGLGR
FRVSAFTQRDAAGMVLRRIETNIPSSEELHLPPVLKDLIMYKRGLVMFVGATGTGKSTSLAALIRHRNENSNGHIITIED
PIEFVHPHKGCIITQREVGLDTESYEVALKNTLRQAPDVILIGEVRTKETMQHAITFAETGHLCLCTLHANNANQALDRI
LHFFPEEMHGQIFMDLSLNLRGIVAQQLVKRADGKGRYPAIEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALNSADSRNEVRLMIKLGAENANFDNDEMHLTETDENNGSLYR

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=504047 IVG45_RS14550 WP_196434528.1 3138725..3139858(-) (pilU) [Methylomonas sp. LL1]
ATGGATTTCAAAGCACTATTAGCCTTGATGGTTGAAAAAAAGGCATCGGATTTATTTATTACCGCCGGCAAGCCGCCCTG
TATGAAAATCGACGGTAAGGTGGTGGAAATTTCCAAAAACATTTTGACCGCCGACCAGGCGCTGAAAATCGTTTTGAGCA
TTATGGAGCAACGTCAGAAAGACGAATTCGAACATACCAAGGAATGCCAGTTTGCGATGGGCGTCGAAGGTTTGGGCCGA
TTCCGGGTCAGCGCCTTTACCCAGCGCGATGCCGCAGGCATGGTGTTACGCCGCATCGAAACCAATATTCCGTCTTCGGA
AGAGTTGCATTTGCCGCCGGTTTTGAAAGATCTGATCATGTACAAGCGCGGTCTGGTGATGTTCGTGGGCGCTACCGGTA
CCGGTAAATCGACTTCGTTGGCGGCTTTGATTCGGCACCGCAACGAAAACAGTAACGGCCATATCATCACCATCGAAGAT
CCGATCGAGTTCGTGCATCCACATAAAGGCTGCATCATCACTCAGCGCGAGGTCGGGCTGGATACCGAATCTTACGAAGT
GGCGCTGAAAAACACACTACGGCAAGCGCCGGATGTGATTCTAATCGGTGAGGTCAGGACCAAGGAAACCATGCAGCATG
CTATTACTTTTGCCGAAACAGGACATTTGTGCTTGTGCACGCTGCATGCGAATAATGCCAACCAGGCGCTGGACCGGATT
TTGCATTTCTTCCCGGAAGAGATGCATGGGCAAATATTCATGGATTTGTCATTGAATTTACGCGGCATCGTCGCCCAGCA
ATTGGTCAAGCGTGCCGATGGCAAGGGACGTTATCCGGCCATTGAGATTTTGCTGAACACCCCGTTGGTATCGGATTTAA
TTCGCAAGGGCGAGGTGCATAAACTGAAGGAATTGATGAAAAACTCCCGCGAACATGGCATGCAAACCTTCGATCAGGCC
CTATACGATCTGTATACAGCTGGTAAAATCAGCTACGAAGATGCACTGAATTCGGCCGACTCCAGAAACGAAGTCCGGTT
GATGATCAAACTGGGAGCCGAAAACGCCAATTTCGACAACGACGAGATGCATTTGACCGAGACCGATGAAAACAATGGCA
GCCTGTATCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0NXA5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.946

98.143

0.647
  pilU Acinetobacter baylyi ADP1

62.637

96.552

0.605
  pilU Vibrio cholerae strain A1552

58.286

92.838

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.54

92.308

0.411
  pilT Legionella pneumophila strain Lp02

43.149

90.981

0.393
  pilT Legionella pneumophila strain ERS1305867

43.149

90.981

0.393
  pilT Acinetobacter baumannii D1279779

41.593

89.92

0.374
  pilT Acinetobacter nosocomialis M2

41.593

89.92

0.374
  pilT Acinetobacter baumannii strain A118

41.593

89.92

0.374
  pilT Pseudomonas aeruginosa PAK

41.593

89.92

0.374
  pilT Acinetobacter baylyi ADP1

41.056

90.451

0.371
  pilT Vibrio cholerae strain A1552

41.617

88.594

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

88.594

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.118

89.92

0.361