Detailed information NTG0050203

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: KO11_RS08385
• Genome accession: NC_017660
• Coordinates: 1695780..1696760 (+)
• Length: 326 a.a.
• NCBI ID: WP_001349905.1
• Uniprot ID: A0A376FYN5
• Organism: Escherichia coli KO11FL
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1690780..1701760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KO11_RS08355 (KO11_07970)	yggM	1691295..1692302 (+)	1008	WP_000745217.1	DUF1202 family protein	-
KO11_RS08360 (KO11_07975)	hemW	1692457..1693593 (-)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
KO11_RS08365 (KO11_07980)	rdgB	1693586..1694179 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
KO11_RS08370 (KO11_07985)	yggU	1694187..1694477 (-)	291	WP_001277226.1	DUF167 family protein YggU	-
KO11_RS08375 (KO11_07990)	yggT	1694474..1695040 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
KO11_RS08380 (KO11_07995)	yggS	1695058..1695762 (-)	705	WP_001349904.1	pyridoxal phosphate homeostasis protein	-
KO11_RS08385 (KO11_08000)	pilT	1695780..1696760 (+)	981	WP_001349905.1	type IV pilus twitching motility protein PilT	Machinery gene
KO11_RS08390 (KO11_08005)	ruvX	1696936..1697352 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
KO11_RS08395 (KO11_08010)	yqgE	1697352..1697915 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
KO11_RS08400 (KO11_08015)	gshB	1698024..1698974 (-)	951	WP_000593273.1	glutathione synthase	-
KO11_RS08405 (KO11_08020)	rsmE	1698987..1699718 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
KO11_RS08410 (KO11_08025)	endA	1699798..1700505 (-)	708	WP_000286496.1	deoxyribonuclease I	-
KO11_RS08415 (KO11_08030)	yggI	1700600..1701097 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=50203 KO11_RS08385 WP_001349905.1 1695780..1696760(+) (pilT) [Escherichia coli KO11FL]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=50203 KO11_RS08385 WP_001349905.1 1695780..1696760(+) (pilT) [Escherichia coli KO11FL]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A376FYN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362