NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	A7A19_RS16890	Genome accession	NZ_CP076279
Coordinates	3387422..3388402 (-)	Length	326 a.a.
NCBI ID	WP_001055618.1	Uniprot ID	-
Organism	Escherichia coli strain SA53-3
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3382422..3393402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7A19_RS16860 (A7A19_16815)	yggI	3383085..3383582 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
A7A19_RS16865 (A7A19_16820)	endA	3383677..3384384 (+)	708	WP_000286500.1	deoxyribonuclease I	-
A7A19_RS16870 (A7A19_16825)	rsmE	3384464..3385195 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
A7A19_RS16875 (A7A19_16830)	gshB	3385208..3386158 (+)	951	WP_000593273.1	glutathione synthase	-
A7A19_RS16880 (A7A19_16835)	yqgE	3386267..3386830 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
A7A19_RS16885 (A7A19_16840)	ruvX	3386830..3387246 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
A7A19_RS16890 (A7A19_16845)	pilT	3387422..3388402 (-)	981	WP_001055618.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A7A19_RS16895 (A7A19_16850)	yggS	3388420..3389124 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
A7A19_RS16900 (A7A19_16855)	yggT	3389142..3389708 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
A7A19_RS16905 (A7A19_16860)	yggU	3389705..3389995 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
A7A19_RS16910 (A7A19_16865)	rdgB	3390003..3390596 (+)	594	WP_001174751.1	XTP/dITP diphosphatase	-
A7A19_RS16915 (A7A19_16870)	hemW	3390589..3391725 (+)	1137	WP_000239939.1	radical SAM family heme chaperone HemW	-
A7A19_RS16920 (A7A19_16875)	-	3392039..3393025 (+)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 35899.09 Da Isoelectric Point: 5.5860

                                >NTDB_id=501753 A7A19_RS16890 WP_001055618.1 3387422..3388402(-) (pilT) [Escherichia coli strain SA53-3]

MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG

SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE

YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR

VGEGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=501753 A7A19_RS16890 WP_001055618.1 3387422..3388402(-) (pilT) [Escherichia coli strain SA53-3]

ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG

ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG

ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC

AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG

TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT

CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA

TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG

GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG

CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA

TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT

GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA

AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG

GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362

Detailed information NTG0501753