Detailed information NTG0050139

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: I02_RS16085
• Genome accession: NC_017651
• Coordinates: 3277166..3278146 (-)
• Length: 326 a.a.
• NCBI ID: WP_001327409.1
• Uniprot ID: A0A3T4X286
• Organism: Escherichia coli str. 'clone D i2'
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3272166..3283146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I02_RS16055 (i02_3248)	yggI	3272831..3273328 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
I02_RS16060 (i02_3249)	endA	3273423..3274130 (+)	708	WP_001327408.1	deoxyribonuclease I	-
I02_RS16065 (i02_3250)	rsmE	3274210..3274941 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
I02_RS16070 (i02_3251)	gshB	3274954..3275904 (+)	951	WP_000593261.1	glutathione synthase	-
I02_RS16075 (i02_3252)	yqgE	3276013..3276576 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
I02_RS16080 (i02_3253)	ruvX	3276576..3276992 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
I02_RS16085 (i02_3254)	pilT	3277166..3278146 (-)	981	WP_001327409.1	type IV pilus twitching motility protein PilT	Machinery gene
I02_RS16090 (i02_3255)	yggS	3278164..3278868 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
I02_RS16095 (i02_3256)	yggT	3278886..3279452 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
I02_RS16100 (i02_3257)	yggU	3279449..3279739 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
I02_RS16105 (i02_3258)	rdgB	3279747..3280340 (+)	594	WP_001174738.1	XTP/dITP diphosphatase	-
I02_RS16110 (i02_3259)	hemW	3280333..3281469 (+)	1137	WP_000239981.1	radical SAM family heme chaperone HemW	-
I02_RS16115 (i02_3260)	yggM	3281538..3282545 (-)	1008	WP_000745192.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36089.34 Da
• Isoelectric Point: 5.9975

>NTDB_id=50139 I02_RS16085 WP_001327409.1 3277166..3278146(-) (pilT) [Escherichia coli str. 'clone D i2']
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDALDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=50139 I02_RS16085 WP_001327409.1 3277166..3278146(-) (pilT) [Escherichia coli str. 'clone D i2']
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCTGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A3T4X286

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365