Detailed information NTG0050052

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ECW_RS16450
• Genome accession: NC_017635
• Coordinates: 3295251..3296231 (-)
• Length: 326 a.a.
• NCBI ID: WP_001349905.1
• Uniprot ID: A0A376FYN5
• Organism: Escherichia coli W
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3290251..3301231

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECW_RS16420 (ECW_m3202)	yggI	3290914..3291411 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
ECW_RS16425 (ECW_m3203)	endA	3291506..3292213 (+)	708	WP_000286496.1	deoxyribonuclease I	-
ECW_RS16430 (ECW_m3204)	rsmE	3292293..3293024 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ECW_RS16435 (ECW_m3205)	gshB	3293037..3293987 (+)	951	WP_000593273.1	glutathione synthase	-
ECW_RS16440 (ECW_m3206)	yqgE	3294096..3294659 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
ECW_RS16445 (ECW_m3207)	ruvX	3294659..3295075 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
ECW_RS16450 (ECW_m3208)	pilT	3295251..3296231 (-)	981	WP_001349905.1	type IV pilus twitching motility protein PilT	Machinery gene
ECW_RS16455 (ECW_m3209)	yggS	3296249..3296953 (+)	705	WP_001349904.1	pyridoxal phosphate homeostasis protein	-
ECW_RS16460 (ECW_m3210)	yggT	3296971..3297537 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ECW_RS16465 (ECW_m3211)	yggU	3297534..3297824 (+)	291	WP_001277226.1	DUF167 family protein YggU	-
ECW_RS16470 (ECW_m3212)	rdgB	3297832..3298425 (+)	594	WP_001174743.1	XTP/dITP diphosphatase	-
ECW_RS16475 (ECW_m3213)	hemW	3298418..3299554 (+)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
ECW_RS16480 (ECW_m3214)	yggM	3299709..3300716 (-)	1008	WP_000745217.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=50052 ECW_RS16450 WP_001349905.1 3295251..3296231(-) (pilT) [Escherichia coli W]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=50052 ECW_RS16450 WP_001349905.1 3295251..3296231(-) (pilT) [Escherichia coli W]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A376FYN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362