Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JFX93_RS18715 Genome accession   NZ_CP075627
Coordinates   3848152..3849132 (-) Length   326 a.a.
NCBI ID   WP_001055618.1    Uniprot ID   -
Organism   Escherichia coli strain P15-385     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3843152..3854132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFX93_RS18685 (JFX93_018685) yggI 3843815..3844312 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  JFX93_RS18690 (JFX93_018690) endA 3844407..3845114 (+) 708 WP_000286500.1 deoxyribonuclease I -
  JFX93_RS18695 (JFX93_018695) rsmE 3845194..3845925 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JFX93_RS18700 (JFX93_018700) gshB 3845938..3846888 (+) 951 WP_000593273.1 glutathione synthase -
  JFX93_RS18705 (JFX93_018705) yqgE 3846997..3847560 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  JFX93_RS18710 (JFX93_018710) ruvX 3847560..3847976 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  JFX93_RS18715 (JFX93_018715) pilT 3848152..3849132 (-) 981 WP_001055618.1 type IV pilus twitching motility protein PilT Machinery gene
  JFX93_RS18720 (JFX93_018720) yggS 3849150..3849854 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JFX93_RS18725 (JFX93_018725) yggT 3849872..3850438 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JFX93_RS18730 (JFX93_018730) yggU 3850435..3850725 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  JFX93_RS18735 (JFX93_018735) rdgB 3850733..3851326 (+) 594 WP_001174751.1 XTP/dITP diphosphatase -
  JFX93_RS18740 (JFX93_018740) hemW 3851319..3852455 (+) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  JFX93_RS18745 (JFX93_018745) - 3852771..3853757 (+) 987 WP_000784004.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35899.09 Da        Isoelectric Point: 5.5860

>NTDB_id=499119 JFX93_RS18715 WP_001055618.1 3848152..3849132(-) (pilT) [Escherichia coli strain P15-385]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=499119 JFX93_RS18715 WP_001055618.1 3848152..3849132(-) (pilT) [Escherichia coli strain P15-385]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362