Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ISN30_RS16655 Genome accession   NZ_CP064005
Coordinates   3865716..3866753 (-) Length   345 a.a.
NCBI ID   WP_003471666.1    Uniprot ID   A0A0K2ZRY0
Organism   Xanthomonas translucens pv. translucens strain XtKm7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3860716..3871753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN30_RS16635 (ISN30_16625) - 3861144..3861710 (-) 567 WP_003471660.1 YqgE/AlgH family protein -
  ISN30_RS16640 (ISN30_16630) - 3861818..3863626 (+) 1809 WP_003478084.1 DUF4153 domain-containing protein -
  ISN30_RS16645 (ISN30_16635) - 3863642..3864181 (+) 540 WP_060748302.1 DNA-3-methyladenine glycosylase I -
  ISN30_RS16650 (ISN30_16640) pilU 3864437..3865567 (-) 1131 WP_003478080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISN30_RS16655 (ISN30_16645) pilT 3865716..3866753 (-) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  ISN30_RS16660 (ISN30_16650) - 3866878..3867528 (+) 651 WP_060748304.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISN30_RS16665 (ISN30_16655) - 3868012..3869061 (+) 1050 WP_003478077.1 hypothetical protein -
  ISN30_RS16670 (ISN30_16660) proC 3869191..3870033 (+) 843 WP_065898891.1 pyrroline-5-carboxylate reductase -
  ISN30_RS16675 (ISN30_16665) - 3870048..3870503 (+) 456 WP_003478073.1 DUF4426 domain-containing protein -
  ISN30_RS16680 (ISN30_16670) soxR 3870642..3871079 (-) 438 WP_003478071.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38599.24 Da        Isoelectric Point: 6.6650

>NTDB_id=498511 ISN30_RS16655 WP_003471666.1 3865716..3866753(-) (pilT) [Xanthomonas translucens pv. translucens strain XtKm7]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=498511 ISN30_RS16655 WP_003471666.1 3865716..3866753(-) (pilT) [Xanthomonas translucens pv. translucens strain XtKm7]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCACTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATCCCGTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCTTTGCGCGCGGTGATCTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGCCGCACCGCAGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGACTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZRY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397