Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   IGI28_RS02645 Genome accession   NZ_CP075484
Coordinates   443122..444246 (+) Length   374 a.a.
NCBI ID   WP_000228531.1    Uniprot ID   A7ZSH4
Organism   Escherichia coli strain NT1N25     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 438122..449246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI28_RS02620 mscL 438248..438658 (-) 411 WP_000022439.1 large-conductance mechanosensitive channel protein MscL -
  IGI28_RS02625 trkA 438788..440164 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  IGI28_RS02630 rsmB 440186..441475 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  IGI28_RS02635 fmt 441521..442468 (-) 948 WP_000004432.1 methionyl-tRNA formyltransferase -
  IGI28_RS02640 def 442483..442992 (-) 510 WP_000114984.1 peptide deformylase -
  IGI28_RS02645 dprA 443122..444246 (+) 1125 WP_000228531.1 DNA-protecting protein DprA Machinery gene
  IGI28_RS02650 smg 444218..444691 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  IGI28_RS02655 yrdD 444720..445262 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  IGI28_RS02660 tsaC 445267..445839 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  IGI28_RS02665 aroE 445844..446662 (+) 819 WP_000451211.1 shikimate dehydrogenase -
  IGI28_RS02670 yrdB 446659..446916 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  IGI28_RS02675 yrdA 446892..447446 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40986.98 Da        Isoelectric Point: 6.3122

>NTDB_id=498510 IGI28_RS02645 WP_000228531.1 443122..444246(+) (dprA) [Escherichia coli strain NT1N25]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLMTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=498510 IGI28_RS02645 WP_000228531.1 443122..444246(+) (dprA) [Escherichia coli strain NT1N25]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGATGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393