Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ISN30_RS16650 Genome accession   NZ_CP064005
Coordinates   3864437..3865567 (-) Length   376 a.a.
NCBI ID   WP_003478080.1    Uniprot ID   A0A1C3TLF5
Organism   Xanthomonas translucens pv. translucens strain XtKm7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3859437..3870567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN30_RS16625 (ISN30_16615) - 3859687..3860634 (-) 948 WP_003478088.1 aspartate carbamoyltransferase catalytic subunit -
  ISN30_RS16630 (ISN30_16620) ruvX 3860654..3861151 (-) 498 WP_065898892.1 Holliday junction resolvase RuvX -
  ISN30_RS16635 (ISN30_16625) - 3861144..3861710 (-) 567 WP_003471660.1 YqgE/AlgH family protein -
  ISN30_RS16640 (ISN30_16630) - 3861818..3863626 (+) 1809 WP_003478084.1 DUF4153 domain-containing protein -
  ISN30_RS16645 (ISN30_16635) - 3863642..3864181 (+) 540 WP_060748302.1 DNA-3-methyladenine glycosylase I -
  ISN30_RS16650 (ISN30_16640) pilU 3864437..3865567 (-) 1131 WP_003478080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISN30_RS16655 (ISN30_16645) pilT 3865716..3866753 (-) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  ISN30_RS16660 (ISN30_16650) - 3866878..3867528 (+) 651 WP_060748304.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISN30_RS16665 (ISN30_16655) - 3868012..3869061 (+) 1050 WP_003478077.1 hypothetical protein -
  ISN30_RS16670 (ISN30_16660) proC 3869191..3870033 (+) 843 WP_065898891.1 pyrroline-5-carboxylate reductase -
  ISN30_RS16675 (ISN30_16665) - 3870048..3870503 (+) 456 WP_003478073.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41956.32 Da        Isoelectric Point: 6.8811

>NTDB_id=498510 ISN30_RS16650 WP_003478080.1 3864437..3865567(-) (pilU) [Xanthomonas translucens pv. translucens strain XtKm7]
MSIIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPSPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=498510 ISN30_RS16650 WP_003478080.1 3864437..3865567(-) (pilU) [Xanthomonas translucens pv. translucens strain XtKm7]
ATGAGCATCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGATGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCAATCTCCTTCGCCGAGACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAATTTCTTTCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAATATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGAGCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGTGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3TLF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.312

98.138

0.641
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367