Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ISN31_RS01440 Genome accession   NZ_CP064004
Coordinates   338373..339410 (+) Length   345 a.a.
NCBI ID   WP_003471666.1    Uniprot ID   A0A0K2ZRY0
Organism   Xanthomonas translucens pv. translucens strain XtKm8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333373..344410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN31_RS01415 (ISN31_01415) soxR 334047..334484 (+) 438 WP_003478071.1 redox-sensitive transcriptional activator SoxR -
  ISN31_RS01420 (ISN31_01420) - 334623..335078 (-) 456 WP_003478073.1 DUF4426 domain-containing protein -
  ISN31_RS01425 (ISN31_01425) proC 335093..335935 (-) 843 WP_065898891.1 pyrroline-5-carboxylate reductase -
  ISN31_RS01430 (ISN31_01430) - 336065..337114 (-) 1050 WP_003478077.1 hypothetical protein -
  ISN31_RS01435 (ISN31_01435) - 337598..338248 (-) 651 WP_060748304.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISN31_RS01440 (ISN31_01440) pilT 338373..339410 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  ISN31_RS01445 (ISN31_01445) pilU 339559..340689 (+) 1131 WP_003478080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISN31_RS01450 (ISN31_01450) - 340945..341484 (-) 540 WP_060748302.1 DNA-3-methyladenine glycosylase I -
  ISN31_RS01455 (ISN31_01455) - 341500..343308 (-) 1809 WP_003478084.1 DUF4153 domain-containing protein -
  ISN31_RS01460 (ISN31_01460) - 343416..343982 (+) 567 WP_003471660.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38599.24 Da        Isoelectric Point: 6.6650

>NTDB_id=498489 ISN31_RS01440 WP_003471666.1 338373..339410(+) (pilT) [Xanthomonas translucens pv. translucens strain XtKm8]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=498489 ISN31_RS01440 WP_003471666.1 338373..339410(+) (pilT) [Xanthomonas translucens pv. translucens strain XtKm8]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCACTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATCCCGTCCGAAGTGCTGACCCTGGA
AGACCTCGGTTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCTCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCTTTGCGCGCGGTGATCTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGCCGCACCGCAGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGACTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZRY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397