Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ISN32_RS02855 Genome accession   NZ_CP064003
Coordinates   680921..681337 (+) Length   138 a.a.
NCBI ID   WP_128809130.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. translucens strain XtKm9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 675921..686337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN32_RS02835 (ISN32_02835) - 676316..677650 (+) 1335 WP_058360086.1 HAMP domain-containing sensor histidine kinase -
  ISN32_RS02840 (ISN32_02840) coaE 677773..678393 (-) 621 WP_058360087.1 dephospho-CoA kinase -
  ISN32_RS02845 (ISN32_02845) - 678407..679270 (-) 864 WP_058359048.1 A24 family peptidase -
  ISN32_RS02850 (ISN32_02850) pilC 679277..680536 (-) 1260 WP_064522057.1 type II secretion system F family protein Machinery gene
  ISN32_RS02855 (ISN32_02855) pilA 680921..681337 (+) 417 WP_128809130.1 pilin Machinery gene
  ISN32_RS02860 (ISN32_02860) - 681429..681797 (-) 369 WP_153476696.1 hypothetical protein -
  ISN32_RS02865 (ISN32_02865) pilB 682060..683796 (+) 1737 WP_058360091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISN32_RS02870 (ISN32_02870) - 683806..684423 (-) 618 WP_003477095.1 Fic family protein -
  ISN32_RS02875 (ISN32_02875) - 684420..684602 (-) 183 WP_003477093.1 YhfG family protein -
  ISN32_RS02880 (ISN32_02880) pilR 684791..686179 (-) 1389 WP_058360092.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14459.79 Da        Isoelectric Point: 8.4798

>NTDB_id=498471 ISN32_RS02855 WP_128809130.1 680921..681337(+) (pilA) [Xanthomonas translucens pv. translucens strain XtKm9]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVAAGLSEITPGKTQYEVMVNEGKASTITPDLIGLKTSSRCTI
TTPNPGETGSINCALLNVSPKVAGKSILWTRAADGTWTCSTDLAATDKPKYAPATCQG

Nucleotide


Download         Length: 417 bp        

>NTDB_id=498471 ISN32_RS02855 WP_128809130.1 680921..681337(+) (pilA) [Xanthomonas translucens pv. translucens strain XtKm9]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
GCCTGCTTATCAGAATTATGTTGCTAAATCACAGGTGGCAGCTGGTCTTTCGGAGATCACGCCTGGTAAAACTCAGTACG
AGGTGATGGTTAATGAAGGGAAAGCTTCGACTATTACTCCTGATCTGATTGGCCTTAAGACTTCAAGCCGTTGCACCATC
ACGACTCCCAATCCTGGCGAAACTGGCTCCATCAATTGCGCTTTGTTGAATGTAAGTCCTAAGGTTGCGGGAAAGAGTAT
CCTTTGGACGCGTGCCGCTGACGGTACTTGGACCTGTTCAACTGATCTCGCTGCGACCGATAAGCCTAAGTACGCGCCGG
CTACGTGTCAGGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.593

97.826

0.514
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.175

99.275

0.478
  pilA Pseudomonas aeruginosa PAK

41.83

100

0.464
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.588

98.551

0.449
  pilA Ralstonia pseudosolanacearum GMI1000

35.583

100

0.42
  comP Acinetobacter baylyi ADP1

36.129

100

0.406
  pilA/pilA1 Eikenella corrodens VA1

36.184

100

0.399
  pilA Vibrio cholerae C6706

39.568

100

0.399
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.568

100

0.399
  pilA Vibrio cholerae strain A1552

39.568

100

0.399
  pilA2 Legionella pneumophila strain ERS1305867

36.806

100

0.384
  pilA Vibrio parahaemolyticus RIMD 2210633

40.152

95.652

0.384
  pilE Neisseria gonorrhoeae strain FA1090

33.548

100

0.377
  pilA2 Legionella pneumophila str. Paris

36.17

100

0.37