Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ISN32_RS02865 Genome accession   NZ_CP064003
Coordinates   682060..683796 (+) Length   578 a.a.
NCBI ID   WP_058360091.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. translucens strain XtKm9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677060..688796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN32_RS02840 (ISN32_02840) coaE 677773..678393 (-) 621 WP_058360087.1 dephospho-CoA kinase -
  ISN32_RS02845 (ISN32_02845) - 678407..679270 (-) 864 WP_058359048.1 A24 family peptidase -
  ISN32_RS02850 (ISN32_02850) pilC 679277..680536 (-) 1260 WP_064522057.1 type II secretion system F family protein Machinery gene
  ISN32_RS02855 (ISN32_02855) pilA 680921..681337 (+) 417 WP_128809130.1 pilin Machinery gene
  ISN32_RS02860 (ISN32_02860) - 681429..681797 (-) 369 WP_153476696.1 hypothetical protein -
  ISN32_RS02865 (ISN32_02865) pilB 682060..683796 (+) 1737 WP_058360091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISN32_RS02870 (ISN32_02870) - 683806..684423 (-) 618 WP_003477095.1 Fic family protein -
  ISN32_RS02875 (ISN32_02875) - 684420..684602 (-) 183 WP_003477093.1 YhfG family protein -
  ISN32_RS02880 (ISN32_02880) pilR 684791..686179 (-) 1389 WP_058360092.1 sigma-54 dependent transcriptional regulator Regulator
  ISN32_RS02885 (ISN32_02885) - 686254..687867 (-) 1614 WP_003477089.1 ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62969.29 Da        Isoelectric Point: 6.2656

>NTDB_id=498472 ISN32_RS02865 WP_058360091.1 682060..683796(+) (pilB) [Xanthomonas translucens pv. translucens strain XtKm9]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKYQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGVDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTHLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=498472 ISN32_RS02865 WP_058360091.1 682060..683796(+) (pilB) [Xanthomonas translucens pv. translucens strain XtKm9]
ATGAACGCTGTGACATCCGCCAATCTTGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGATGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGTCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGATA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGTATCCTCAACGACGAGACCCGCAACATTTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCATCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.754

98.27

0.538
  pilB Acinetobacter baumannii D1279779

53.873

98.27

0.529
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.047

99.827

0.49
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.466

97.924

0.455
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.421

90.311

0.365
  pilF Thermus thermophilus HB27

40.385

89.965

0.363