Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INQ43_RS09770 Genome accession   NZ_CP063658
Coordinates   2155805..2156842 (-) Length   345 a.a.
NCBI ID   WP_043957729.1    Uniprot ID   -
Organism   Lysobacter sp. H23M47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2150805..2161842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ43_RS09740 (INQ43_09740) - 2150925..2151875 (-) 951 WP_194034079.1 aspartate carbamoyltransferase catalytic subunit -
  INQ43_RS09745 (INQ43_09745) ruvX 2151968..2152441 (-) 474 Protein_1899 Holliday junction resolvase RuvX -
  INQ43_RS09750 (INQ43_09750) - 2152438..2153004 (-) 567 WP_194034081.1 YqgE/AlgH family protein -
  INQ43_RS09755 (INQ43_09755) - 2153007..2153624 (+) 618 WP_194034082.1 DNA-3-methyladenine glycosylase I -
  INQ43_RS09760 (INQ43_09760) - 2153661..2154488 (+) 828 WP_194036307.1 META and DUF4377 domain-containing protein -
  INQ43_RS09765 (INQ43_09765) pilU 2154592..2155746 (-) 1155 WP_194034084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ43_RS09770 (INQ43_09770) pilT 2155805..2156842 (-) 1038 WP_043957729.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ43_RS09775 (INQ43_09775) proC 2157157..2158008 (+) 852 WP_194036308.1 pyrroline-5-carboxylate reductase -
  INQ43_RS09780 (INQ43_09780) - 2158052..2158528 (+) 477 WP_194036309.1 DUF4426 domain-containing protein -
  INQ43_RS09785 (INQ43_09785) - 2158540..2159334 (+) 795 WP_194037648.1 cobalamin-binding protein -
  INQ43_RS09790 (INQ43_09790) pdxH 2159401..2160003 (-) 603 WP_194036310.1 pyridoxamine 5'-phosphate oxidase -
  INQ43_RS09795 (INQ43_09795) - 2160064..2160267 (-) 204 WP_194034088.1 dodecin family protein -
  INQ43_RS09800 (INQ43_09800) - 2160391..2160942 (+) 552 WP_194036311.1 shikimate kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38479.19 Da        Isoelectric Point: 6.7297

>NTDB_id=496168 INQ43_RS09770 WP_043957729.1 2155805..2156842(-) (pilT) [Lysobacter sp. H23M47]
MDIAELLAFSVKNKASDLHLSAGMPPMIRVDGDVRRINIPALDHKTVHALVYDIMSDKQRRDFEEFLETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLATPRIFKDLIDQPQGLILVTGPTGSGKSTTLAAMIDHINKTEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQNSGMMTLDQHL
QDLVKRGLILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=496168 INQ43_RS09770 WP_043957729.1 2155805..2156842(-) (pilT) [Lysobacter sp. H23M47]
ATGGATATTGCCGAGCTGCTGGCGTTTTCAGTCAAGAACAAGGCATCCGATCTGCATCTGTCGGCCGGCATGCCGCCGAT
GATCCGCGTGGACGGCGATGTCCGCCGGATCAACATCCCGGCACTGGACCACAAGACGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTTTGAAGAGTTCCTGGAAACCGACTTCTCGTTCGAGATCCCCGGCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACGCTTGA
GGACCTGGCCACGCCGCGGATCTTCAAGGACCTGATCGACCAGCCGCAGGGTCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGACCGAGTACGGGCACATCCTGTCGGTGGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAATGCCTGATCAACCAGCGCGAGGTCCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGTGAGGATCCCGACTACATCCTGGTGGGTGAGCTGCGCGATCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGCCACCGTGCATACCAGCTCGGCGGCCAAGACCATCGACCGAATC
ATCGATGTGTTCCCGGCGGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCAGCCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGCCGCACCGCGGCGTGGGAGATCATGGTCGGCACGCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCCATCCAGACCGGCCAGAACAGCGGCATGATGACCCTGGACCAGCACCTC
CAGGATCTGGTGAAACGCGGGCTGATCCTGCGCCCGCAGGCGCGCGAATACGCCAAGGACAAGCGGCTGTTCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.809

98.841

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

41.818

95.652

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.923

94.203

0.386