Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INQ43_RS09765 Genome accession   NZ_CP063658
Coordinates   2154592..2155746 (-) Length   384 a.a.
NCBI ID   WP_194034084.1    Uniprot ID   -
Organism   Lysobacter sp. H23M47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2149592..2160746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ43_RS09740 (INQ43_09740) - 2150925..2151875 (-) 951 WP_194034079.1 aspartate carbamoyltransferase catalytic subunit -
  INQ43_RS09745 (INQ43_09745) ruvX 2151968..2152441 (-) 474 Protein_1899 Holliday junction resolvase RuvX -
  INQ43_RS09750 (INQ43_09750) - 2152438..2153004 (-) 567 WP_194034081.1 YqgE/AlgH family protein -
  INQ43_RS09755 (INQ43_09755) - 2153007..2153624 (+) 618 WP_194034082.1 DNA-3-methyladenine glycosylase I -
  INQ43_RS09760 (INQ43_09760) - 2153661..2154488 (+) 828 WP_194036307.1 META and DUF4377 domain-containing protein -
  INQ43_RS09765 (INQ43_09765) pilU 2154592..2155746 (-) 1155 WP_194034084.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ43_RS09770 (INQ43_09770) pilT 2155805..2156842 (-) 1038 WP_043957729.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ43_RS09775 (INQ43_09775) proC 2157157..2158008 (+) 852 WP_194036308.1 pyrroline-5-carboxylate reductase -
  INQ43_RS09780 (INQ43_09780) - 2158052..2158528 (+) 477 WP_194036309.1 DUF4426 domain-containing protein -
  INQ43_RS09785 (INQ43_09785) - 2158540..2159334 (+) 795 WP_194037648.1 cobalamin-binding protein -
  INQ43_RS09790 (INQ43_09790) pdxH 2159401..2160003 (-) 603 WP_194036310.1 pyridoxamine 5'-phosphate oxidase -
  INQ43_RS09795 (INQ43_09795) - 2160064..2160267 (-) 204 WP_194034088.1 dodecin family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42812.12 Da        Isoelectric Point: 6.4191

>NTDB_id=496167 INQ43_RS09765 WP_194034084.1 2154592..2155746(-) (pilU) [Lysobacter sp. H23M47]
MNTTQSTNETIDFTSFLKLMAHQKASDLFITAGMPPSMKVNGVLQPITKSPLTPQQSRDLVLNVMNPSQREEFEKTHEAN
FAIGLANVGRFRVSCFYQRNQVGMVLRRIETRIPTIDELGLPQVLKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQN
STGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHA
NNANQAMDRMINFFPEDRRNQLLMDLSLNLKGVVAQQLVPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKEST
QLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGNAQTLAAGMDGVEVAEIRD

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=496167 INQ43_RS09765 WP_194034084.1 2154592..2155746(-) (pilU) [Lysobacter sp. H23M47]
ATGAACACGACCCAGAGCACGAACGAGACGATCGACTTCACGTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCCGA
TCTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTCAACGGCGTGCTGCAGCCGATCACCAAGAGCCCGTTGACGC
CGCAGCAAAGCCGCGACCTGGTGCTCAACGTGATGAATCCCTCGCAGCGAGAGGAGTTCGAGAAGACACACGAGGCGAAC
TTCGCGATCGGGCTGGCCAATGTCGGCCGCTTCCGCGTCAGCTGTTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCG
CCGGATCGAGACCAGGATCCCGACCATCGACGAGCTGGGCCTGCCGCAGGTGCTCAAGACGCTGGCGATGACCAAGCGCG
GGATCATCCTGTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAAC
TCGACCGGCCACATCATCACCATCGAGGATCCGATCGAGTTCGTGCACAAGCACGAGGGTTGCATCATTACCCAGCGTGA
GGTCGGCATCGATACCGACAGCTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCATGATCGGCG
AGGTGCGCACCCGCGAGGGCATGGACCACGCGATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCACGCC
AACAACGCCAACCAGGCGATGGACCGGATGATCAACTTCTTCCCCGAGGACCGGCGCAACCAGTTGCTGATGGACCTGTC
GCTGAACCTCAAGGGCGTGGTGGCCCAGCAACTGGTTCCCACCCCGGACGGCAAGGCGCGCCGGGTGGCGATGGAAATCC
TGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACC
CAGTTGGGCATGAAGACCTTCGACCAGGCACTTTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCACTGCG
CTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCAATGCGCAGACCCTCGCCGCGGGCA
TGGACGGCGTGGAAGTGGCCGAGATCCGCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

95.573

0.651
  pilU Acinetobacter baylyi ADP1

63.866

92.969

0.594
  pilU Vibrio cholerae strain A1552

54.213

92.708

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.288

95.052

0.411