Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INQ41_RS10255 Genome accession   NZ_CP063656
Coordinates   2276789..2277826 (-) Length   345 a.a.
NCBI ID   WP_193984180.1    Uniprot ID   A0A7S6UEV9
Organism   Novilysobacter ciconiae strain H21R20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2271789..2282826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ41_RS10225 (INQ41_10225) - 2271836..2272786 (-) 951 WP_193984168.1 aspartate carbamoyltransferase catalytic subunit -
  INQ41_RS10230 (INQ41_10230) ruvX 2272870..2273352 (-) 483 Protein_1997 Holliday junction resolvase RuvX -
  INQ41_RS10235 (INQ41_10235) - 2273349..2273915 (-) 567 WP_193984173.1 YqgE/AlgH family protein -
  INQ41_RS10240 (INQ41_10240) - 2273918..2274535 (+) 618 WP_193984175.1 DNA-3-methyladenine glycosylase I -
  INQ41_RS10245 (INQ41_10245) - 2274572..2275399 (+) 828 WP_193984176.1 META and DUF4377 domain-containing protein -
  INQ41_RS10250 (INQ41_10250) pilU 2275577..2276731 (-) 1155 WP_193984178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ41_RS10255 (INQ41_10255) pilT 2276789..2277826 (-) 1038 WP_193984180.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ41_RS10260 (INQ41_10260) proC 2278130..2278981 (+) 852 WP_193984181.1 pyrroline-5-carboxylate reductase -
  INQ41_RS10265 (INQ41_10265) - 2279025..2279501 (+) 477 WP_193984183.1 DUF4426 domain-containing protein -
  INQ41_RS10270 (INQ41_10270) - 2279513..2280307 (+) 795 WP_193987328.1 cobalamin-binding protein -
  INQ41_RS10275 (INQ41_10275) pdxH 2280365..2280967 (-) 603 WP_193984185.1 pyridoxamine 5'-phosphate oxidase -
  INQ41_RS10280 (INQ41_10280) - 2281028..2281231 (-) 204 WP_193984187.1 dodecin family protein -
  INQ41_RS10285 (INQ41_10285) - 2281355..2281906 (+) 552 WP_193984189.1 shikimate kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38475.20 Da        Isoelectric Point: 6.7297

>NTDB_id=496128 INQ41_RS10255 WP_193984180.1 2276789..2277826(-) (pilT) [Novilysobacter ciconiae strain H21R20]
MDIAELLAFSVKNKASDLHLSAGMPPMIRVDGDVRRINIPALDHKTVHALVYDIMSDKQRRDFEEFLETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLATPRIFKDLIDQPQGLILVTGPTGSGKSTTLAAMIDHINKPEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQNSGMMTLDQHL
QDLVKRGLILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=496128 INQ41_RS10255 WP_193984180.1 2276789..2277826(-) (pilT) [Novilysobacter ciconiae strain H21R20]
ATGGATATCGCCGAGCTGCTGGCGTTTTCAGTCAAGAACAAGGCATCCGATCTGCACCTGTCGGCCGGCATGCCGCCGAT
GATCCGCGTGGACGGCGATGTCCGCCGGATCAACATCCCGGCGCTGGACCACAAGACGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGGGACTTCGAGGAGTTCCTGGAAACCGACTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGTGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTATTCCGCACCATTCCCTCCGAGGTGTTGACGCTTGA
GGACCTGGCCACGCCGCGGATCTTCAAAGACCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGCCCGAGTACGGGCACATCCTGTCGGTGGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTCCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGTGAGGACCCCGACTACATTCTGGTCGGCGAGCTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACGGGACACTTGGTGTTCGCCACGGTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCCATGCTGTCCGAGTCGCTGCGTGCGGTGATCAGCCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGCACCGCGGCGTGGGAAATCATGGTCGGCACGCCAGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCCATCCAGACCGGCCAGAACAGCGGCATGATGACCCTTGACCAGCATCTG
CAGGACCTGGTGAAACGCGGGCTGATCCTGCGTCCGCAGGCCCGCGAATACGCCAAGGACAAGCGGCTGTTCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6UEV9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

64.809

98.841

0.641
  pilT Neisseria gonorrhoeae MS11

64.516

98.841

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.818

95.652

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.923

94.203

0.386