Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INQ41_RS10250 Genome accession   NZ_CP063656
Coordinates   2275577..2276731 (-) Length   384 a.a.
NCBI ID   WP_193984178.1    Uniprot ID   A0A7S6UEY3
Organism   Novilysobacter ciconiae strain H21R20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270577..2281731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ41_RS10225 (INQ41_10225) - 2271836..2272786 (-) 951 WP_193984168.1 aspartate carbamoyltransferase catalytic subunit -
  INQ41_RS10230 (INQ41_10230) ruvX 2272870..2273352 (-) 483 Protein_1997 Holliday junction resolvase RuvX -
  INQ41_RS10235 (INQ41_10235) - 2273349..2273915 (-) 567 WP_193984173.1 YqgE/AlgH family protein -
  INQ41_RS10240 (INQ41_10240) - 2273918..2274535 (+) 618 WP_193984175.1 DNA-3-methyladenine glycosylase I -
  INQ41_RS10245 (INQ41_10245) - 2274572..2275399 (+) 828 WP_193984176.1 META and DUF4377 domain-containing protein -
  INQ41_RS10250 (INQ41_10250) pilU 2275577..2276731 (-) 1155 WP_193984178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ41_RS10255 (INQ41_10255) pilT 2276789..2277826 (-) 1038 WP_193984180.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ41_RS10260 (INQ41_10260) proC 2278130..2278981 (+) 852 WP_193984181.1 pyrroline-5-carboxylate reductase -
  INQ41_RS10265 (INQ41_10265) - 2279025..2279501 (+) 477 WP_193984183.1 DUF4426 domain-containing protein -
  INQ41_RS10270 (INQ41_10270) - 2279513..2280307 (+) 795 WP_193987328.1 cobalamin-binding protein -
  INQ41_RS10275 (INQ41_10275) pdxH 2280365..2280967 (-) 603 WP_193984185.1 pyridoxamine 5'-phosphate oxidase -
  INQ41_RS10280 (INQ41_10280) - 2281028..2281231 (-) 204 WP_193984187.1 dodecin family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42850.21 Da        Isoelectric Point: 6.4191

>NTDB_id=496127 INQ41_RS10250 WP_193984178.1 2275577..2276731(-) (pilU) [Novilysobacter ciconiae strain H21R20]
MNTTQSTNETIDFTSFLKLMAHQKASDLFITAGMPPSMKVNGVLQPITKSPLTPQQSRDLVLNVMNPAQREEFEKTHEAN
FAIGLANVGRFRVSCFYQRNQVGMVLRRIETRIPTIDELNLPPVLKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQN
STGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHA
NNANQAMDRMINFFPEDRRNQLLMDLSLNLKGVIAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKEST
QLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGNAQTLAAGMDGVEVAEIRD

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=496127 INQ41_RS10250 WP_193984178.1 2275577..2276731(-) (pilU) [Novilysobacter ciconiae strain H21R20]
ATGAACACGACCCAGAGCACCAACGAGACGATCGACTTCACGTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCCGA
CCTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTCAACGGCGTCCTGCAGCCGATCACCAAGAGCCCGCTGACGC
CGCAGCAAAGCCGCGACTTGGTGCTCAACGTGATGAATCCGGCCCAGCGGGAGGAGTTCGAGAAGACCCACGAGGCGAAC
TTCGCGATCGGGCTGGCCAACGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCG
CCGGATCGAGACCCGGATCCCGACCATCGACGAGCTGAACCTGCCGCCGGTGCTGAAGACGCTGGCGATGACCAAGCGCG
GGATCATCCTGTTCGTCGGCGCCACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAAC
TCGACCGGCCACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGA
GGTCGGCATCGACACCGACAGCTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCATGATCGGCG
AGGTGCGCACCCGCGAGGGCATGGACCACGCGATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCACGCC
AACAACGCCAACCAGGCGATGGACCGGATGATCAACTTCTTCCCCGAGGACCGGCGCAACCAGTTGCTGATGGACCTGTC
GCTGAACCTGAAGGGCGTGATCGCCCAGCAGCTGATCCCGACCCCGGACGGCAAGGCGCGCCGGGTCGCGATGGAGATCC
TGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACC
CAGCTGGGCATGAAGACCTTCGACCAGGCGCTTTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCCTTGCG
CTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTTGCACAGGGCGGCAACGCGCAGACCCTCGCCGCGGGCA
TGGACGGCGTGGAAGTGGCCGAGATCCGCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6UEY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

95.573

0.646
  pilU Acinetobacter baylyi ADP1

64.706

92.969

0.602
  pilU Vibrio cholerae strain A1552

54.775

92.708

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.74

95.052

0.406