Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KIL99_RS04420 Genome accession   NZ_CP074951
Coordinates   904971..905951 (+) Length   326 a.a.
NCBI ID   WP_001055634.1    Uniprot ID   -
Organism   Escherichia coli strain 03_3503     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899971..910951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL99_RS04390 (KIL99_04365) - 900349..901335 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  KIL99_RS04395 (KIL99_04370) hemW 901648..902784 (-) 1137 WP_000239919.1 radical SAM family heme chaperone HemW -
  KIL99_RS04400 (KIL99_04375) rdgB 902777..903370 (-) 594 WP_001174742.1 XTP/dITP diphosphatase -
  KIL99_RS04405 (KIL99_04380) yggU 903378..903668 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  KIL99_RS04410 (KIL99_04385) yggT 903665..904231 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  KIL99_RS04415 (KIL99_04390) yggS 904249..904953 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  KIL99_RS04420 (KIL99_04395) pilT 904971..905951 (+) 981 WP_001055634.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KIL99_RS04425 (KIL99_04400) ruvX 906127..906543 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  KIL99_RS04430 (KIL99_04405) yqgE 906543..907106 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  KIL99_RS04435 (KIL99_04410) gshB 907215..908165 (-) 951 WP_000593273.1 glutathione synthase -
  KIL99_RS04440 (KIL99_04415) rsmE 908178..908909 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KIL99_RS04445 (KIL99_04420) endA 908989..909696 (-) 708 WP_000286500.1 deoxyribonuclease I -
  KIL99_RS04450 (KIL99_04425) yggI 909791..910288 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.5860

>NTDB_id=495990 KIL99_RS04420 WP_001055634.1 904971..905951(+) (pilT) [Escherichia coli strain 03_3503]
MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=495990 KIL99_RS04420 WP_001055634.1 904971..905951(+) (pilT) [Escherichia coli strain 03_3503]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGTCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTAGCGGCGATGGTTGGCTATCTCTATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.125

98.16

0.374
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362