Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IM816_RS13195 Genome accession   NZ_CP063231
Coordinates   3059630..3060667 (-) Length   345 a.a.
NCBI ID   WP_072321676.1    Uniprot ID   A0A1K1NMS7
Organism   Luteibacter flocculans strain EIF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3054630..3065667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM816_RS13175 (IM816_13155) - 3054735..3055661 (+) 927 WP_072321672.1 aspartate carbamoyltransferase catalytic subunit -
  IM816_RS13180 (IM816_13160) - 3055763..3057526 (+) 1764 WP_250338427.1 hypothetical protein -
  IM816_RS13185 (IM816_13165) - 3057763..3058128 (+) 366 WP_250338428.1 BON domain-containing protein -
  IM816_RS13190 (IM816_13170) pilU 3058339..3059502 (-) 1164 WP_072321675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IM816_RS13195 (IM816_13175) pilT 3059630..3060667 (-) 1038 WP_072321676.1 type IV pilus twitching motility protein PilT Machinery gene
  IM816_RS13200 (IM816_13180) proC 3060793..3061617 (+) 825 WP_072321677.1 pyrroline-5-carboxylate reductase -
  IM816_RS13205 (IM816_13185) - 3061614..3062189 (+) 576 WP_250338429.1 YggT family protein -
  IM816_RS13210 (IM816_13190) - 3062235..3064340 (-) 2106 WP_250338430.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38390.96 Da        Isoelectric Point: 6.7837

>NTDB_id=493982 IM816_RS13195 WP_072321676.1 3059630..3060667(-) (pilT) [Luteibacter flocculans strain EIF3]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNHNRGAGGVFRTIPSEVLTLEDLGAPKIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHVNKTEYGHILTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRLAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQNL
ADLVKRGLITRQEASTYAKNKEGFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=493982 IM816_RS13195 WP_072321676.1 3059630..3060667(-) (pilT) [Luteibacter flocculans strain EIF3]
ATGGATATCGCCGAACTCCTTGCCTTCTCCGTGAAGAACAAGGCCTCCGACCTGCACCTTTCCGCGGGTCTGCCGCCGAT
GATTCGCGTCGACGGCGACGTCCGGCGCATCAACATCCCGGCTCTGGAACACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTATGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCGGGCCTGGCCCGC
TTCCGCGTCAACGCCTTCAACCACAACCGTGGCGCGGGCGGCGTGTTCCGTACCATTCCATCCGAGGTGCTGACCCTGGA
AGACCTCGGTGCGCCGAAGATTTTCCGCGAGCTGATCGAGCAGCCGCAGGGCCTCATTCTCGTGACGGGCCCTACCGGCT
CGGGCAAGTCCACGACGCTGGCCGCCATGGTCGATCACGTCAACAAGACCGAATACGGCCATATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCTCAGAAGTGTCTGGTGAACCAGCGCGAGGTGCATCGCGACACGCATGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACTACATCCTGGTCGGTGAGTTGCGCGATCTGGAAACCATTCGCCTCG
CCCTGACCGCCGCCGAGACGGGGCATCTGGTGTTCGGCACGCTGCATACGTCGTCCGCGGCGAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCAGGCGAGAAGCCGATGGTCCGCTCGATGCTCTCCGAATCGTTGCGCGCAGTGATCTCCCAGTC
GCTGCTGAAGAAGGTGGGCGGCGGGCGACTGGCGGCACACGAGATCATGGTGGGTATTCCCGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCCTCCATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGATCAGAACCTG
GCCGACCTGGTGAAGCGCGGCCTCATCACCCGCCAGGAAGCCTCGACCTACGCGAAGAACAAGGAAGGCTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1K1NMS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

65.217

100

0.652
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.755

95.942

0.487
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

39.595

100

0.397
  pilU Acinetobacter baylyi ADP1

38.905

100

0.391