Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IM816_RS13190 Genome accession   NZ_CP063231
Coordinates   3058339..3059502 (-) Length   387 a.a.
NCBI ID   WP_072321675.1    Uniprot ID   A0A1K1NML1
Organism   Luteibacter flocculans strain EIF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3053339..3064502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM816_RS13165 (IM816_13145) - 3053690..3054256 (+) 567 WP_072321670.1 YqgE/AlgH family protein -
  IM816_RS13170 (IM816_13150) ruvX 3054270..3054689 (+) 420 WP_250340762.1 Holliday junction resolvase RuvX -
  IM816_RS13175 (IM816_13155) - 3054735..3055661 (+) 927 WP_072321672.1 aspartate carbamoyltransferase catalytic subunit -
  IM816_RS13180 (IM816_13160) - 3055763..3057526 (+) 1764 WP_250338427.1 hypothetical protein -
  IM816_RS13185 (IM816_13165) - 3057763..3058128 (+) 366 WP_250338428.1 BON domain-containing protein -
  IM816_RS13190 (IM816_13170) pilU 3058339..3059502 (-) 1164 WP_072321675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IM816_RS13195 (IM816_13175) pilT 3059630..3060667 (-) 1038 WP_072321676.1 type IV pilus twitching motility protein PilT Machinery gene
  IM816_RS13200 (IM816_13180) proC 3060793..3061617 (+) 825 WP_072321677.1 pyrroline-5-carboxylate reductase -
  IM816_RS13205 (IM816_13185) - 3061614..3062189 (+) 576 WP_250338429.1 YggT family protein -
  IM816_RS13210 (IM816_13190) - 3062235..3064340 (-) 2106 WP_250338430.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43453.73 Da        Isoelectric Point: 6.9971

>NTDB_id=493981 IM816_RS13190 WP_072321675.1 3058339..3059502(-) (pilU) [Luteibacter flocculans strain EIF3]
MSDFDFTSFLKLMVHKQASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPAQREEFEKTHECQFAISASG
IGRFRVSCFYQRNCVGMVLRRIESKIPTPEELNLPPIIKQLAMTKRGIIIFVGGTGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQRELGIDTDSWENALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRILHFFPEDRRQQLFMDLSLNLKGIVAQQLIPTPDGKARRVAVEVLLGTPLAQDYIRQGEVHKLKELMRDSNQLGMRTF
DQSLVELYHQGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVEIDKTTDRNTFGGMLHR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=493981 IM816_RS13190 WP_072321675.1 3058339..3059502(-) (pilU) [Luteibacter flocculans strain EIF3]
ATGAGCGACTTCGATTTCACCTCATTTCTCAAGCTGATGGTGCACAAGCAGGCATCGGACTTGTTCATCACTGCTGGCGT
GGCACCTTCCATGAAGGTGCAGGGGCGTATCGTCCCCATCACCCAGAGCCCGCTGTCCGCGCAGCAGTCGCGCGACATGG
TGCTGAACGTGATGACGCCTGCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCCAGTTTGCGATCAGTGCCTCGGGC
ATCGGTCGTTTCCGCGTGTCGTGCTTTTACCAGCGCAATTGCGTGGGCATGGTGCTGCGCCGGATCGAATCGAAGATCCC
GACGCCGGAAGAACTGAACCTGCCGCCGATCATCAAGCAACTGGCGATGACCAAGCGCGGCATCATCATCTTCGTAGGCG
GTACCGGTACCGGTAAGTCCACCTCGCTCGCGTCGATGATCGGCTACCGTAACCAGAACTCGACGGGACACATCATCACG
ATCGAGGATCCGATCGAGTACGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAACTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACGCTGCGCCAGGCACCCGACGTGATCATGATCGGCGAGGTGCGCACGCGGGAGACCA
TGGAGCATGCGATCAACTTCTCCGAAACCGGTCACCTCTGCCTGTGTACGCTGCATGCGAACAACGCCAACCAGGCGCTG
GATCGCATCCTGCATTTCTTCCCCGAAGATCGGCGCCAGCAGCTCTTCATGGATCTCTCGCTGAACCTGAAAGGCATCGT
GGCGCAGCAGCTCATTCCGACGCCCGATGGCAAGGCACGTCGCGTGGCGGTGGAAGTGCTGCTCGGTACGCCGCTCGCCC
AGGATTACATCCGCCAGGGTGAGGTCCACAAGCTCAAGGAACTGATGCGCGATTCCAACCAGCTTGGCATGCGCACGTTC
GACCAGAGCCTCGTGGAGCTGTACCACCAGGGCGAGATCTCCTACGAGGACGCCCTGCGCCACGCGGACAGCTCCAACGA
AGTGCGCTTGCGCATCAAGCTCGCCCAGGGCGGCGACGCGCATACGCTGTCGCAAGGTCTGGAAGGCGTGGAGATCGACA
AGACCACCGATCGCAACACCTTCGGCGGCATGCTGCATCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1K1NML1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.845

96.641

0.646
  pilU Acinetobacter baylyi ADP1

63.764

91.99

0.587
  pilU Vibrio cholerae strain A1552

51.622

95.607

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.247

96.124

0.377