Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INR79_RS01865 Genome accession   NZ_CP063199
Coordinates   395498..396535 (+) Length   345 a.a.
NCBI ID   WP_004398311.1    Uniprot ID   A0AAV2VQ50
Organism   Vibrio sp. SCSIO 43132     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390498..401535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01830 (INR79_01830) hemW 390704..391879 (-) 1176 WP_223095786.1 radical SAM family heme chaperone HemW -
  INR79_RS01835 (INR79_01835) - 391886..392485 (-) 600 WP_223095788.1 XTP/dITP diphosphatase -
  INR79_RS01840 (INR79_01840) - 392552..392983 (-) 432 WP_223095790.1 DUF4426 domain-containing protein -
  INR79_RS01845 (INR79_01845) yggU 393007..393297 (-) 291 WP_223095792.1 DUF167 family protein YggU -
  INR79_RS01850 (INR79_01850) - 393297..393854 (-) 558 WP_004398314.1 YggT family protein -
  INR79_RS01855 (INR79_01855) proC 393884..394702 (-) 819 WP_223095794.1 pyrroline-5-carboxylate reductase -
  INR79_RS01860 (INR79_01860) - 394764..395474 (-) 711 WP_022591346.1 YggS family pyridoxal phosphate-dependent enzyme -
  INR79_RS01865 (INR79_01865) pilT 395498..396535 (+) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  INR79_RS01870 (INR79_01870) pilU 396544..397650 (+) 1107 WP_223095796.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INR79_RS01875 (INR79_01875) ruvX 397733..398158 (-) 426 WP_022549508.1 Holliday junction resolvase RuvX -
  INR79_RS01880 (INR79_01880) - 398212..398775 (-) 564 WP_004409298.1 YqgE/AlgH family protein -
  INR79_RS01885 (INR79_01885) gshB 398802..399749 (-) 948 WP_223095798.1 glutathione synthase -
  INR79_RS01890 (INR79_01890) rsmE 399764..400495 (-) 732 WP_223095800.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INR79_RS01895 (INR79_01895) - 400601..401302 (-) 702 WP_223095802.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38005.26 Da        Isoelectric Point: 6.2687

>NTDB_id=493792 INR79_RS01865 WP_004398311.1 395498..396535(+) (pilT) [Vibrio sp. SCSIO 43132]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFTHADVHRLVFDIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTEVPSLEQISAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGTDKDMVRSMLSESLRAVISQKLLKRLGGGRAACHEIMLATPAIRNLIREDKVAQMYSVIQTGGAMGMQTTEQHA
KQLIAQGVVDADEVRTKLESDSMGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=493792 INR79_RS01865 WP_004398311.1 395498..396535(+) (pilT) [Vibrio sp. SCSIO 43132]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGAGTTCCTCCAAT
GGTCCGCGTAGACGGCGATGTACGTAAGTTGGGCGTGCCTGCTTTTACTCATGCTGATGTTCACCGGTTGGTTTTCGATA
TTATGAACGACGCGCAACGCGCTGAGTTCGAAGAAAAACTGGAAACGGACTTCTCTTTTGAACTGCCTAATGTTGGTCGC
TTCCGTGTGAACGCTTTTAATCAGTCACGCGGGTGCTCGGCGGTATTTCGAACCATTCCAACCGAAGTGCCAAGCCTGGA
GCAAATCAGTGCGCCTGATATTTTTACTCAAATTTCTAATTATGAGAAAGGGTTAGTGCTGGTAACCGGTCCTACGGGTT
CGGGTAAATCCACCACACTTGCCGCCATGGTTGACCACATCAACCGCAATCACAACAAACACATTCTTACGATCGAAGAT
CCAATTGAGTTTGTACACCAAAGTAATAAATGCTTGATCAACCAACGTGAAGTTCATCGCGATACGCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGATCCGGATGTGATTCTGGTGGGTGAGCTTCGTGATAAGGAAACCATCAGCCTTG
CATTGACGGCTGCGGAAACGGGTCACTTGGTATTTGGTACCTTGCACACCAGCAGTGCAGCCAAAACCGTCGACCGTATT
ATCGATGTGTTCCCGGGCACCGACAAAGACATGGTTCGCTCGATGCTCTCTGAATCGCTACGTGCGGTTATCTCTCAAAA
GCTGCTTAAGCGTTTGGGTGGTGGTCGTGCCGCCTGTCACGAAATCATGCTGGCAACACCTGCGATTCGAAACCTGATTC
GTGAAGACAAAGTCGCTCAGATGTATTCGGTTATCCAGACGGGTGGCGCTATGGGGATGCAGACCACAGAGCAGCATGCC
AAACAGTTGATTGCTCAAGGGGTGGTCGATGCTGACGAGGTACGCACCAAACTTGAATCCGATTCTATGGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.797

100

0.858
  pilT Vibrio cholerae strain A1552

85.797

100

0.858
  pilT Acinetobacter baumannii D1279779

72.917

97.391

0.71
  pilT Acinetobacter baumannii strain A118

72.917

97.391

0.71
  pilT Acinetobacter nosocomialis M2

72.619

97.391

0.707
  pilT Acinetobacter baylyi ADP1

70

98.551

0.69
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

63.689

100

0.641
  pilT Neisseria gonorrhoeae MS11

63.401

100

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.812

0.49
  pilU Vibrio cholerae strain A1552

41.846

94.203

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371