Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INR79_RS01870 Genome accession   NZ_CP063199
Coordinates   396544..397650 (+) Length   368 a.a.
NCBI ID   WP_223095796.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43132     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391544..402650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INR79_RS01835 (INR79_01835) - 391886..392485 (-) 600 WP_223095788.1 XTP/dITP diphosphatase -
  INR79_RS01840 (INR79_01840) - 392552..392983 (-) 432 WP_223095790.1 DUF4426 domain-containing protein -
  INR79_RS01845 (INR79_01845) yggU 393007..393297 (-) 291 WP_223095792.1 DUF167 family protein YggU -
  INR79_RS01850 (INR79_01850) - 393297..393854 (-) 558 WP_004398314.1 YggT family protein -
  INR79_RS01855 (INR79_01855) proC 393884..394702 (-) 819 WP_223095794.1 pyrroline-5-carboxylate reductase -
  INR79_RS01860 (INR79_01860) - 394764..395474 (-) 711 WP_022591346.1 YggS family pyridoxal phosphate-dependent enzyme -
  INR79_RS01865 (INR79_01865) pilT 395498..396535 (+) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  INR79_RS01870 (INR79_01870) pilU 396544..397650 (+) 1107 WP_223095796.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INR79_RS01875 (INR79_01875) ruvX 397733..398158 (-) 426 WP_022549508.1 Holliday junction resolvase RuvX -
  INR79_RS01880 (INR79_01880) - 398212..398775 (-) 564 WP_004409298.1 YqgE/AlgH family protein -
  INR79_RS01885 (INR79_01885) gshB 398802..399749 (-) 948 WP_223095798.1 glutathione synthase -
  INR79_RS01890 (INR79_01890) rsmE 399764..400495 (-) 732 WP_223095800.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INR79_RS01895 (INR79_01895) - 400601..401302 (-) 702 WP_223095802.1 endonuclease -
  INR79_RS01900 (INR79_01900) - 401390..401890 (-) 501 WP_223095804.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41282.22 Da        Isoelectric Point: 5.2025

>NTDB_id=493793 INR79_RS01870 WP_223095796.1 396544..397650(+) (pilU) [Vibrio sp. SCSIO 43132]
MELNQVLSEMVSLKASDIYITVGAPVLLRVHGELQPLGEPLTEESAFHLLDTMMDEERRAEFRKERESNFAIVRDVGRFR
VSAFFQRELPGAVLRRIETQIPTFEELKLPDVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNQCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGQGRHGVFEILLNTPRVSDLIRRGELHELKATMAKSNEAGMKTFDQCLY
ELLSADKITEQDAMHSADSANDLRLMMKTEQGGDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=493793 INR79_RS01870 WP_223095796.1 396544..397650(+) (pilU) [Vibrio sp. SCSIO 43132]
ATGGAACTGAACCAAGTTTTATCAGAAATGGTGTCTCTGAAGGCATCAGACATCTACATCACAGTGGGTGCACCTGTCTT
GTTGCGTGTTCACGGAGAACTGCAGCCATTGGGTGAACCACTAACCGAGGAGTCTGCCTTTCATCTGCTCGATACTATGA
TGGATGAAGAGCGTCGCGCGGAATTTCGTAAAGAAAGAGAGTCCAACTTTGCCATCGTTCGAGATGTTGGACGTTTTCGT
GTGAGTGCGTTCTTCCAGCGTGAACTGCCTGGTGCGGTACTACGCCGAATAGAGACGCAAATCCCTACCTTCGAAGAGCT
TAAATTACCGGATGTTCTGCAGGATCTTGCTATCGCTAAACGAGGTTTAGTGCTGGTGGTTGGTGCGACCGGCTCAGGTA
AGTCCACCACGATGGCTGCGATGACGGGCTTTCGAAATCAGAATCGCACAGGTCATATCCTGACGGTGGAAGACCCGATT
GAATTTGTTCACGAGCATAACCAGTGCATCATCACCCAGCGTGAAGTGGGTTTGGATACCGAGAGCTATGAAGTCGCACT
GAAGAATTCACTCCGTCAGGCGCCAGACATGATTCTGATTGGTGAAATTCGCTCCCGTGAAACCATGGAATATGCCATGA
CTTTTGCGGAAACCGGTCACCTCTGCATGGCAACACTGCACGCTAACAACGCAAACCAGGCGTTGGAACGTATTCTTCAC
CTAGTGCCAAAAGAGCAGAAAGAGCAGTTCCTGTTTGATTTATCCATGAACTTGCGCGGTGTGATAGCGCAGCAACTGAT
TCGCGATAAGAGCGGGCAAGGGCGCCACGGTGTATTCGAAATCCTTCTGAATACCCCAAGGGTGTCCGACCTGATTCGTC
GCGGTGAGCTGCACGAGCTGAAAGCTACGATGGCGAAGTCCAACGAGGCGGGAATGAAAACCTTCGATCAGTGTTTGTAT
GAGCTTCTGTCGGCAGACAAGATCACCGAGCAGGATGCAATGCACAGCGCTGACTCTGCGAATGATTTGAGGTTGATGAT
GAAAACCGAGCAAGGCGGAGATTTCTCAGGTGGCGCGCTTGATGGTGTGAAAATAGACATGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

80.109

99.728

0.799
  pilU Pseudomonas stutzeri DSM 10701

55.645

100

0.562
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter nosocomialis M2

41.471

92.391

0.383
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

41.176

92.391

0.38
  pilT Acinetobacter baumannii strain A118

41.176

92.391

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Legionella pneumophila strain Lp02

40.118

92.12

0.37
  pilT Legionella pneumophila strain ERS1305867

40.118

92.12

0.37
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361