Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F7R26_RS17275 Genome accession   NZ_CP062803
Coordinates   3763123..3764166 (-) Length   347 a.a.
NCBI ID   WP_150985964.1    Uniprot ID   -
Organism   Cupriavidus basilensis strain DSM 11853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3758123..3769166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R26_RS17245 (F7R26_017240) - 3759047..3759433 (+) 387 WP_150985969.1 CidA/LrgA family protein -
  F7R26_RS17250 (F7R26_017245) - 3759430..3760152 (+) 723 WP_150985968.1 LrgB family protein -
  F7R26_RS17255 (F7R26_017250) - 3760226..3760591 (+) 366 WP_150985967.1 VOC family protein -
  F7R26_RS17260 (F7R26_017255) - 3760646..3761335 (+) 690 WP_150985966.1 helix-turn-helix transcriptional regulator -
  F7R26_RS17265 (F7R26_017260) - 3761373..3761864 (-) 492 WP_150985965.1 glutathione peroxidase -
  F7R26_RS17270 (F7R26_017265) pilU 3761949..3763088 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F7R26_RS17275 (F7R26_017270) pilT 3763123..3764166 (-) 1044 WP_150985964.1 type IV pilus twitching motility protein PilT Machinery gene
  F7R26_RS17280 (F7R26_017275) - 3764230..3764958 (+) 729 WP_150985963.1 YggS family pyridoxal phosphate-dependent enzyme -
  F7R26_RS17285 (F7R26_017280) proC 3765004..3765828 (+) 825 WP_150985962.1 pyrroline-5-carboxylate reductase -
  F7R26_RS17290 (F7R26_017285) ubiA 3765894..3766751 (-) 858 WP_150985961.1 4-hydroxybenzoate octaprenyltransferase -
  F7R26_RS17295 (F7R26_017290) - 3766879..3767364 (-) 486 WP_035867478.1 Dps family protein -
  F7R26_RS17300 (F7R26_017295) - 3767442..3767636 (-) 195 WP_150985960.1 hypothetical protein -
  F7R26_RS17305 (F7R26_017300) - 3767714..3769159 (-) 1446 WP_150985959.1 catalase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38427.15 Da        Isoelectric Point: 7.0151

>NTDB_id=491483 F7R26_RS17275 WP_150985964.1 3763123..3764166(-) (pilT) [Cupriavidus basilensis strain DSM 11853]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMGHKDVHAMVYDIMSDTQRKQYEERLEIDFSFEIASLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDHDMGHILTVED
PIEFVHTSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGIINYNDARAIAKNPDSFTG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=491483 F7R26_RS17275 WP_150985964.1 3763123..3764166(-) (pilT) [Cupriavidus basilensis strain DSM 11853]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTCCATCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATCCACGGCGACATGCGCCGCATCAATGTGGCCGCGATGGGCCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGCAATATGAGGAACGGCTGGAAATCGACTTTTCATTCGAGATCGCCAGCCTCTCGCGT
TTCCGGGTCAATGCCTACAACACGCAGCGCGGCGCCGCCGCCGTGTTCCGGACCATCCCTTCCAAGGTACTGACGCTGGA
AGACCTGCGCGCGCCGGCGGTATTCGCTGACCTGTGCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTGGACCACCGCAACGATCACGACATGGGCCACATCCTCACAGTGGAGGAC
CCGATCGAGTTCGTGCACACGTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCCTTTGCCAA
CGCGCTGCGCTCCGCGCTGCGTGAGGATCCCGACGTGGTGCTGGTGGGGGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGCCACGCTGCACACGAGCTCGGCGGCCAAGACCATCGACCGGGTT
GTCGACGTGTTCCCGCCCGAAGAAAAAGACATGGTGCGCACCATGTTGTCGGAATCGCTGGAAGCGGTGATTTCGCAAAC
GCTGCTCAAGACGCGCGATGGCAACGGCCGTACGGCGGCCCACGAGATCATGATCGCCACGCCGGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCCTTGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCGGCATCATCAACTACAACGATGCGCGCGCCATTGCCAAGAACCCCGACTCATTCACGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Acinetobacter baylyi ADP1

70.435

99.424

0.7
  pilT Pseudomonas stutzeri DSM 10701

69.565

99.424

0.692
  pilT Pseudomonas aeruginosa PAK

68.696

99.424

0.683
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Neisseria gonorrhoeae MS11

66.474

99.712

0.663
  pilT Neisseria meningitidis 8013

66.474

99.712

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.118

0.637
  pilT Vibrio cholerae strain A1552

65.579

97.118

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

99.424

0.484
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.299

96.542

0.389