Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   F7R26_RS17270 Genome accession   NZ_CP062803
Coordinates   3761949..3763088 (-) Length   379 a.a.
NCBI ID   WP_043349449.1    Uniprot ID   A0A0C4YJM3
Organism   Cupriavidus basilensis strain DSM 11853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3756949..3768088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R26_RS17240 (F7R26_017235) glcF 3757704..3758969 (+) 1266 WP_150985970.1 glycolate oxidase subunit GlcF -
  F7R26_RS17245 (F7R26_017240) - 3759047..3759433 (+) 387 WP_150985969.1 CidA/LrgA family protein -
  F7R26_RS17250 (F7R26_017245) - 3759430..3760152 (+) 723 WP_150985968.1 LrgB family protein -
  F7R26_RS17255 (F7R26_017250) - 3760226..3760591 (+) 366 WP_150985967.1 VOC family protein -
  F7R26_RS17260 (F7R26_017255) - 3760646..3761335 (+) 690 WP_150985966.1 helix-turn-helix transcriptional regulator -
  F7R26_RS17265 (F7R26_017260) - 3761373..3761864 (-) 492 WP_150985965.1 glutathione peroxidase -
  F7R26_RS17270 (F7R26_017265) pilU 3761949..3763088 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F7R26_RS17275 (F7R26_017270) pilT 3763123..3764166 (-) 1044 WP_150985964.1 type IV pilus twitching motility protein PilT Machinery gene
  F7R26_RS17280 (F7R26_017275) - 3764230..3764958 (+) 729 WP_150985963.1 YggS family pyridoxal phosphate-dependent enzyme -
  F7R26_RS17285 (F7R26_017280) proC 3765004..3765828 (+) 825 WP_150985962.1 pyrroline-5-carboxylate reductase -
  F7R26_RS17290 (F7R26_017285) ubiA 3765894..3766751 (-) 858 WP_150985961.1 4-hydroxybenzoate octaprenyltransferase -
  F7R26_RS17295 (F7R26_017290) - 3766879..3767364 (-) 486 WP_035867478.1 Dps family protein -
  F7R26_RS17300 (F7R26_017295) - 3767442..3767636 (-) 195 WP_150985960.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41980.17 Da        Isoelectric Point: 6.3709

>NTDB_id=491482 F7R26_RS17270 WP_043349449.1 3761949..3763088(-) (pilU) [Cupriavidus basilensis strain DSM 11853]
MLDRESAAKYINDLLELMVGNRGSDLFITADFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQAGEFDGSRECNFA
ISVPNAGRFRVSAFIQQGKAGMVVRTINTRIPSVEDLDLPPALHDIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTDSWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRKGQKGRVPAVEIMLGTPLVADLIFKGEIHALKEVIKKSREQ
GMVSFDQALFDLYEAGKITYEDALRNADSLNDLRLMIKLHGTMDRETDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=491482 F7R26_RS17270 WP_043349449.1 3761949..3763088(-) (pilU) [Cupriavidus basilensis strain DSM 11853]
ATGCTCGATCGCGAATCCGCTGCCAAGTACATCAACGACCTGCTCGAGCTGATGGTGGGCAACCGCGGCTCGGACCTGTT
CATCACCGCGGATTTTCCGCCGGCCATCAAGGTCGACGGCAAGATCACGCCAGTCTCGCAGCAGCCGCTCAACCCCACCC
AGGCGCTTGGCCTGGTGCGCTCCATCATGAACGAGCGGCAGGCGGGCGAGTTCGACGGCAGCCGCGAGTGCAACTTCGCC
ATCTCCGTGCCCAACGCCGGGCGCTTTCGCGTCTCCGCGTTCATCCAGCAGGGCAAGGCGGGCATGGTGGTGCGTACCAT
CAATACGCGCATTCCTTCGGTGGAAGACCTCGACCTGCCACCCGCGCTGCATGACATCGTGATGTCAAAGCGCGGACTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCTCGCTTGCGGCCATGCTCGATCACCGCAACGCGCATTCGTAC
GGGCACATCATCACCATCGAGGACCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTTACGCAGCGCGAGGTCGG
CATCGATACCGACTCCTGGCACGTGGCGTTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGATCGGGAAACCATGGAGTACGCGATGCAGTACGCCGAAACGGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCCATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTCAA
CCTGAAGGCGATGGTGTCGCAGCGCCTGTTGCCGCGCAAGGGGCAGAAGGGCCGGGTGCCGGCCGTGGAGATCATGCTCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCATGCGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTGTCGTTCGACCAGGCGCTGTTCGATCTCTACGAGGCCGGCAAGATCACCTACGAAGACGCGCTGCGCAATGC
CGACTCCCTCAACGACCTGCGCCTGATGATCAAGCTGCACGGCACCATGGACCGCGAGACCGACCTGGGCGCCGGCACCG
AACACCTGAATGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4YJM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.173

93.14

0.588
  pilU Acinetobacter baylyi ADP1

57.382

94.723

0.544
  pilU Vibrio cholerae strain A1552

53.719

95.778

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Acinetobacter baylyi ADP1

43.363

89.446

0.388
  pilT Legionella pneumophila strain Lp02

44.144

87.863

0.388
  pilT Legionella pneumophila strain ERS1305867

44.144

87.863

0.388
  pilT Acinetobacter nosocomialis M2

43.844

87.863

0.385
  pilT Acinetobacter baumannii D1279779

43.844

87.863

0.385
  pilT Acinetobacter baumannii strain A118

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

43.027

88.918

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361