Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TTJL18_RS00095 Genome accession   NC_017587
Coordinates   17806..18891 (-) Length   361 a.a.
NCBI ID   WP_014628816.1    Uniprot ID   H9ZNN3
Organism   Thermus thermophilus JL-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12806..23891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTJL18_RS00080 (TtJL18_0014) - 14750..15382 (-) 633 WP_014628814.1 histidine phosphatase family protein -
  TTJL18_RS00085 (TtJL18_0015) purM 15379..16380 (-) 1002 WP_008634005.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TTJL18_RS00090 (TtJL18_0016) gatB 16386..17795 (-) 1410 WP_014628815.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TTJL18_RS00095 (TtJL18_0017) pilT 17806..18891 (-) 1086 WP_014628816.1 type IV pilus twitching motility protein PilT Machinery gene
  TTJL18_RS00100 (TtJL18_0018) pilF 18904..21573 (-) 2670 WP_014628817.1 type IV pilus assembly ATPase PilB Machinery gene
  TTJL18_RS00105 (TtJL18_0019) - 21570..22055 (-) 486 WP_014628818.1 YqeG family HAD IIIA-type phosphatase -
  TTJL18_RS00110 (TtJL18_0020) pgeF 22090..22818 (-) 729 WP_014628819.1 peptidoglycan editing factor PgeF -
  TTJL18_RS00115 (TtJL18_0021) surE 22815..23570 (-) 756 WP_014628820.1 5'/3'-nucleotidase SurE -
  TTJL18_RS00120 (TtJL18_0022) - 23627..23833 (-) 207 WP_014628821.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39923.26 Da        Isoelectric Point: 8.0998

>NTDB_id=49110 TTJL18_RS00095 WP_014628816.1 17806..18891(-) (pilT) [Thermus thermophilus JL-18]
MAKAPNVVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRFRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=49110 TTJL18_RS00095 WP_014628816.1 17806..18891(-) (pilT) [Thermus thermophilus JL-18]
ATGGCCAAAGCCCCAAACGTGGTGGACCTTTTGACGCTCGCAGTGGAACGGGGGGCGAGCGACCTCGTGATCACCGTGGG
CCTGCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAACAGCGGATCTTTGAGGAGGAGAAAGAACTAGACTTCTCCTTCAGCCTCCCG
GGCAAGGGGCGCTTCCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGCGGGGTCTTACGGGTGGTGCCGAGCGCTGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCCTTTCCCCCCGGGGCCTGGTCCTGGTCACGG
GGCCCACGGGAAGCGGCAAGAGCACCACGCTGGCCTCCATGATCGACTACATCAACGAGCGCAAACCCGTGCACATCGTG
ACCATTGAGGACCCCATTGAGTTTTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAGACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCTACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGATCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9ZNN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.784

0.665
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

53.254

93.629

0.499
  pilT Neisseria gonorrhoeae MS11

52.522

93.352

0.49
  pilT Legionella pneumophila strain ERS1305867

50.872

95.291

0.485
  pilT Legionella pneumophila strain Lp02

50.872

95.291

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

91.136

0.468
  pilT Vibrio cholerae strain A1552

51.368

91.136

0.468
  pilU Pseudomonas stutzeri DSM 10701

47.522

95.014

0.452
  pilU Vibrio cholerae strain A1552

44.611

92.521

0.413
  pilU Acinetobacter baylyi ADP1

42.105

94.737

0.399

Multiple sequence alignment