Detailed information NTG0049111

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: TTJL18_RS00100
• Genome accession: NC_017587
• Coordinates: 18904..21573 (-)
• Length: 889 a.a.
• NCBI ID: WP_014628817.1
• Uniprot ID: H9ZNN4
• Organism: Thermus thermophilus JL-18
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13904..26573

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TTJL18_RS00080 (TtJL18_0014)	-	14750..15382 (-)	633	WP_014628814.1	histidine phosphatase family protein	-
TTJL18_RS00085 (TtJL18_0015)	purM	15379..16380 (-)	1002	WP_008634005.1	phosphoribosylformylglycinamidine cyclo-ligase	-
TTJL18_RS00090 (TtJL18_0016)	gatB	16386..17795 (-)	1410	WP_014628815.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
TTJL18_RS00095 (TtJL18_0017)	pilT	17806..18891 (-)	1086	WP_014628816.1	type IV pilus twitching motility protein PilT	Machinery gene
TTJL18_RS00100 (TtJL18_0018)	pilF	18904..21573 (-)	2670	WP_014628817.1	type IV pilus assembly ATPase PilB	Machinery gene
TTJL18_RS00105 (TtJL18_0019)	-	21570..22055 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
TTJL18_RS00110 (TtJL18_0020)	pgeF	22090..22818 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
TTJL18_RS00115 (TtJL18_0021)	surE	22815..23570 (-)	756	WP_014628820.1	5'/3'-nucleotidase SurE	-
TTJL18_RS00120 (TtJL18_0022)	-	23627..23833 (-)	207	WP_014628821.1	cold-shock protein	-
TTJL18_RS12865 (TtJL18_0023)	-	23932..24575 (-)	644	Protein_19	transposase	-
TTJL18_RS13270	-	24517..24702 (+)	186	WP_223966770.1	hypothetical protein	-
TTJL18_RS00135 (TtJL18_0024)	-	25022..26311 (+)	1290	WP_014628822.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 97836.92 Da
• Isoelectric Point: 5.4586

>NTDB_id=49111 TTJL18_RS00100 WP_014628817.1 18904..21573(-) (pilF) [Thermus thermophilus JL-18]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKAK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSALLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAAALL
LRSDALRYGAVPIGFQNGKVEVVLSDPRHKEAVAQLLKRPARFYLALPQAWEELFGRAYPEKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRQGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEQDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAQKQVVEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNVSAALIGVLSQRLVRRICDHCKVEVKPDPEVLRRLGL
SEAEIQGARLSKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=49111 TTJL18_RS00100 WP_014628817.1 18904..21573(-) (pilF) [Thermus thermophilus JL-18]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTTGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGCCAAG
GCCCTCCTTCCCGCAGAAAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCCTCCTCTACGCCCTGGCCAAGCACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAAGCGGTGGCCCAGCTCCTCAAGCGGCCCGCCCGCTTCTACCTCGCCCTTCCCCAGGCCTGGGAGGAGC
TTTTCGGCCGGGCCTACCCCGAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAGGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCAGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGCAGGACCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAATATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGCGGAAGGGCCTGA
ACCACGAGGTGGCCCCGGCGGTGGCCACCGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCCAAAAACAGGTGGTGGAAGAGGCTCCAAGTCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAAGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTAAGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCGAAGGGAGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGCCGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTGCGGCTTTCCACCCTGCCCACGGTCTATGGGGAAAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTTACCACCTTTTCCATCCTCAAGCGCATCGCCAC
CCCCGATAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCGC
AGGCGGGCCTCACCTTCGCCCGGGCCCTCCGGGCCTTCCTCCGCCAGGACCCCGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTCAACGTCTCCGCGGCCCTCATCGGCGTCCTCTCGC
AGCGCCTGGTGCGTCGGATCTGCGACCACTGCAAGGTGGAGGTGAAGCCGGACCCCGAGGTTCTAAGGCGCCTCGGGCTT
TCCGAAGCCGAAATCCAAGGGGCCAGGCTCTCCAAGGGCATGGGGTGCGAGCGGTGCGGCGGAACCGGCTACAAGGGCCG
GTATGCCATCCACGAGCTTTTAGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCCGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAGGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (513-779)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

3D structure

• AlphaFold DB: H9ZNN4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	97.75	100	0.978
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.067	100	0.561