Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFU90_RS03900 Genome accession   NZ_CP073953
Coordinates   797269..798249 (+) Length   326 a.a.
NCBI ID   WP_001055627.1    Uniprot ID   A7ZR74
Organism   Escherichia coli strain MB49     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792269..803249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU90_RS03870 (KFU90_03825) yggM 792784..793791 (+) 1008 WP_000745217.1 DUF1202 family protein -
  KFU90_RS03875 (KFU90_03830) hemW 793946..795082 (-) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  KFU90_RS03880 (KFU90_03835) rdgB 795075..795668 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  KFU90_RS03885 (KFU90_03840) yggU 795676..795966 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  KFU90_RS03890 (KFU90_03845) yggT 795963..796529 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  KFU90_RS03895 (KFU90_03850) yggS 796547..797251 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  KFU90_RS03900 (KFU90_03855) pilT 797269..798249 (+) 981 WP_001055627.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFU90_RS03905 (KFU90_03860) ruvX 798425..798841 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  KFU90_RS03910 (KFU90_03865) yqgE 798841..799404 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  KFU90_RS03915 (KFU90_03870) gshB 799513..800463 (-) 951 WP_000593273.1 glutathione synthase -
  KFU90_RS03920 (KFU90_03875) rsmE 800476..801207 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KFU90_RS03925 (KFU90_03880) endA 801287..801994 (-) 708 WP_000286489.1 deoxyribonuclease I -
  KFU90_RS03930 (KFU90_03885) yggI 802089..802586 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.7980

>NTDB_id=488744 KFU90_RS03900 WP_001055627.1 797269..798249(+) (pilT) [Escherichia coli strain MB49]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=488744 KFU90_RS03900 WP_001055627.1 797269..798249(+) (pilT) [Escherichia coli strain MB49]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZR74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362