Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IHE26_RS12885 Genome accession   NZ_CP062196
Coordinates   2857101..2858201 (-) Length   366 a.a.
NCBI ID   WP_010864554.1    Uniprot ID   R8AMI2
Organism   Plesiomonas shigelloides strain P5462     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2852101..2863201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE26_RS12855 (IHE26_12855) rsmE 2852562..2853296 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IHE26_RS12860 (IHE26_12860) gshB 2853306..2854259 (+) 954 WP_047708158.1 glutathione synthase -
  IHE26_RS12865 (IHE26_12865) - 2854473..2855036 (+) 564 WP_192436813.1 YqgE/AlgH family protein -
  IHE26_RS12870 (IHE26_12870) ruvX 2855061..2855480 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  IHE26_RS12875 (IHE26_12875) npr 2855497..2855772 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  IHE26_RS12880 (IHE26_12880) pilU 2856008..2857117 (-) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IHE26_RS12885 (IHE26_12885) pilT 2857101..2858201 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  IHE26_RS12890 (IHE26_12890) - 2858224..2858931 (+) 708 WP_192436814.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHE26_RS12895 (IHE26_12895) proC 2859014..2859832 (+) 819 WP_047708163.1 pyrroline-5-carboxylate reductase -
  IHE26_RS12900 (IHE26_12900) - 2859861..2860415 (+) 555 WP_010864557.1 YggT family protein -
  IHE26_RS12905 (IHE26_12905) yggU 2860427..2860714 (+) 288 WP_084976536.1 DUF167 family protein YggU -
  IHE26_RS12910 (IHE26_12910) - 2860783..2861286 (+) 504 WP_192436815.1 DUF4426 domain-containing protein -
  IHE26_RS12915 (IHE26_12915) - 2861480..2862085 (+) 606 WP_047708167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40421.07 Da        Isoelectric Point: 6.7432

>NTDB_id=486836 IHE26_RS12885 WP_010864554.1 2857101..2858201(-) (pilT) [Plesiomonas shigelloides strain P5462]
MDITELLAFSVKHNASDLHLSAGNPPLIRVDGEVRRVNMPVLDGREVRRLVYDIMNDVQRRDFETEMELDFSFELNGVGR
FRVNAYQQQRGISAALRTIPTDIPTLEQLNAPEVFRNIAMQPRGLVLVTGPTGSGKSTTLAAMLNYVNQHMHHHILTIED
PIEFIHTSNKCLINQREVHRHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGAEKDMVRSMLSESLKAVISQVLLRKEGGGRVAAHEIMLGTPAIRNLIREDKIHQIYSILQTSSRQGMQTLEQAL
NVLTSQGVVSYEEAQRKMNSLMDGGIQNGNTAGGTGAGAMGYGFAG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=486836 IHE26_RS12885 WP_010864554.1 2857101..2858201(-) (pilT) [Plesiomonas shigelloides strain P5462]
ATGGACATAACAGAACTGTTGGCTTTTAGTGTAAAACATAATGCCTCGGATCTGCACCTATCTGCCGGCAACCCACCACT
GATCCGCGTCGACGGGGAAGTGCGTCGGGTCAATATGCCGGTCCTGGATGGCCGTGAAGTGCGCCGTCTGGTGTATGACA
TTATGAACGATGTTCAGCGCCGTGACTTTGAAACTGAGATGGAACTGGACTTTTCGTTCGAACTGAACGGGGTTGGCCGT
TTCCGGGTTAATGCTTATCAGCAGCAACGGGGAATTTCGGCGGCACTGCGTACCATACCTACAGATATACCAACACTGGA
GCAGTTAAACGCACCGGAAGTGTTTCGGAATATTGCCATGCAGCCGCGTGGACTGGTATTGGTGACCGGCCCGACCGGCT
CGGGGAAGTCGACCACCTTGGCGGCGATGCTCAATTATGTCAATCAGCACATGCATCACCACATTCTGACCATTGAAGAC
CCGATCGAATTTATCCACACCAGTAACAAGTGCCTGATTAACCAGCGTGAGGTGCATCGGCATACCCACAGTTTTAATAA
TGCACTGCGCTCGGCGCTGCGTGAAGACCCAGATGTGATTTTGGTGGGTGAGCTGCGCGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCCGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCGATGCTGTCTGAATCGCTCAAAGCGGTTATCTCGCAGGT
ATTGCTGCGCAAAGAAGGCGGCGGACGTGTGGCGGCGCACGAGATCATGCTGGGAACGCCAGCCATTCGTAACTTGATCC
GTGAAGATAAAATTCACCAGATCTATTCGATTTTGCAGACCAGCTCGCGCCAAGGCATGCAGACGCTGGAGCAGGCGCTG
AATGTGCTGACCAGTCAGGGCGTGGTCAGCTACGAAGAAGCGCAGCGCAAGATGAACTCGTTGATGGACGGCGGGATCCA
AAACGGCAATACCGCGGGTGGCACCGGTGCAGGAGCGATGGGCTATGGTTTCGCTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.373

92.35

0.678
  pilT Vibrio cholerae strain A1552

73.373

92.35

0.678
  pilT Pseudomonas stutzeri DSM 10701

71.217

92.077

0.656
  pilT Acinetobacter nosocomialis M2

71.343

91.53

0.653
  pilT Acinetobacter baumannii D1279779

71.045

91.53

0.65
  pilT Acinetobacter baumannii strain A118

71.045

91.53

0.65
  pilT Pseudomonas aeruginosa PAK

70.623

92.077

0.65
  pilT Acinetobacter baylyi ADP1

70.448

91.53

0.645
  pilT Neisseria meningitidis 8013

64.793

92.35

0.598
  pilT Neisseria gonorrhoeae MS11

64.497

92.35

0.596
  pilT Legionella pneumophila strain Lp02

65.269

91.257

0.596
  pilT Legionella pneumophila strain ERS1305867

65.269

91.257

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.087

0.481
  pilU Vibrio cholerae strain A1552

43.023

93.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

43.284

91.53

0.396
  pilU Acinetobacter baylyi ADP1

42.274

93.716

0.396