Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IHE26_RS12880 Genome accession   NZ_CP062196
Coordinates   2856008..2857117 (-) Length   369 a.a.
NCBI ID   WP_010864553.1    Uniprot ID   R8AMH9
Organism   Plesiomonas shigelloides strain P5462     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2851008..2862117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE26_RS12845 (IHE26_12845) - 2851662..2851871 (-) 210 WP_010864545.1 DUF1656 domain-containing protein -
  IHE26_RS12850 (IHE26_12850) - 2851868..2852380 (-) 513 WP_010864546.1 MarR family winged helix-turn-helix transcriptional regulator -
  IHE26_RS12855 (IHE26_12855) rsmE 2852562..2853296 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IHE26_RS12860 (IHE26_12860) gshB 2853306..2854259 (+) 954 WP_047708158.1 glutathione synthase -
  IHE26_RS12865 (IHE26_12865) - 2854473..2855036 (+) 564 WP_192436813.1 YqgE/AlgH family protein -
  IHE26_RS12870 (IHE26_12870) ruvX 2855061..2855480 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  IHE26_RS12875 (IHE26_12875) npr 2855497..2855772 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  IHE26_RS12880 (IHE26_12880) pilU 2856008..2857117 (-) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IHE26_RS12885 (IHE26_12885) pilT 2857101..2858201 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  IHE26_RS12890 (IHE26_12890) - 2858224..2858931 (+) 708 WP_192436814.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHE26_RS12895 (IHE26_12895) proC 2859014..2859832 (+) 819 WP_047708163.1 pyrroline-5-carboxylate reductase -
  IHE26_RS12900 (IHE26_12900) - 2859861..2860415 (+) 555 WP_010864557.1 YggT family protein -
  IHE26_RS12905 (IHE26_12905) yggU 2860427..2860714 (+) 288 WP_084976536.1 DUF167 family protein YggU -
  IHE26_RS12910 (IHE26_12910) - 2860783..2861286 (+) 504 WP_192436815.1 DUF4426 domain-containing protein -
  IHE26_RS12915 (IHE26_12915) - 2861480..2862085 (+) 606 WP_047708167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41485.52 Da        Isoelectric Point: 5.7325

>NTDB_id=486835 IHE26_RS12880 WP_010864553.1 2856008..2857117(-) (pilU) [Plesiomonas shigelloides strain P5462]
MVSLDELLQEMLRQQASDLYVSVDVPAQLRINHELHPLGEALSIETVTELVAQAMGAERFLQFCQQKEGNMALVRPFARF
RVSGFWQRELPGMVIRHIRTDIPTIEELGLPMLTGELAMTRKGLILVVGATGSGKTTTLAAMLGYRNEFDHSHILTVEDP
IEFIHPHKRCLITQREVGMDTESYEVALRNALRQAPDVIQVGEIRTRDTLQHAIGFSETGHLCIATLHANNASQALERMV
HMIPEGSREAFLFDLSNNLRAIIGQQLVPGITPGRRLLVHEILINTPRVTDLLRKNQLYELRDVMASNSEIGMCTFDQSL
YGLYRRGLISYTDALHYAESANDLRLRIRSSGDEPFSPNTFDDVTVDLS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=486835 IHE26_RS12880 WP_010864553.1 2856008..2857117(-) (pilU) [Plesiomonas shigelloides strain P5462]
ATGGTTTCGCTGGATGAGTTATTGCAGGAGATGCTACGTCAGCAGGCGTCGGATCTGTATGTTTCGGTCGATGTGCCCGC
GCAGCTGCGCATCAATCATGAGTTGCACCCACTAGGTGAGGCACTGTCGATTGAGACGGTCACCGAGCTGGTGGCGCAGG
CGATGGGCGCCGAGCGCTTCTTACAGTTTTGTCAGCAAAAAGAGGGCAACATGGCGCTGGTGCGTCCGTTTGCCCGTTTT
CGGGTCTCCGGTTTTTGGCAGCGTGAATTGCCGGGCATGGTGATCCGTCATATTCGTACCGATATTCCGACCATTGAAGA
GCTGGGCTTACCGATGTTAACCGGTGAGCTGGCGATGACGCGCAAAGGTTTGATTTTGGTGGTCGGCGCTACCGGCAGCG
GGAAAACCACCACGCTGGCGGCGATGCTCGGCTATCGCAACGAGTTTGACCATTCACATATTCTGACGGTGGAAGACCCG
ATTGAATTTATCCATCCGCATAAACGCTGTTTAATCACCCAGCGTGAAGTGGGCATGGATACCGAAAGCTATGAAGTCGC
CTTGCGTAACGCGCTGCGCCAAGCACCGGATGTGATTCAAGTTGGCGAGATCCGCACCCGTGACACGCTGCAACATGCGA
TTGGCTTTTCCGAAACCGGCCATCTGTGTATCGCCACCTTGCACGCCAATAACGCCAGCCAAGCACTGGAGCGAATGGTA
CACATGATCCCTGAAGGCAGCCGCGAAGCTTTCTTGTTTGATTTATCCAACAACTTGCGCGCCATTATCGGGCAGCAGCT
GGTGCCGGGGATCACCCCAGGGCGCCGTTTACTGGTGCACGAGATTTTGATCAACACGCCGCGCGTGACCGATTTGCTGC
GTAAAAATCAGTTGTATGAGCTGCGTGATGTGATGGCCAGTAACAGCGAGATTGGCATGTGCACCTTTGACCAATCGCTG
TATGGCTTGTATCGGCGCGGGCTGATTAGCTATACCGATGCGCTGCACTATGCGGAGTCGGCCAATGATTTGCGTTTGCG
GATCCGCAGTAGCGGTGATGAGCCGTTCTCACCGAACACCTTCGATGATGTGACGGTGGATTTAAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

54.768

99.458

0.545
  pilU Pseudomonas stutzeri DSM 10701

51.275

95.664

0.491
  pilU Acinetobacter baylyi ADP1

46.742

95.664

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

99.187

0.39