Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KCG52_RS06150 Genome accession   NZ_CP073114
Coordinates   1296671..1297717 (-) Length   348 a.a.
NCBI ID   WP_070822537.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0048     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291671..1302717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG52_RS06135 (KCG52_06115) - 1291776..1292954 (+) 1179 WP_254343380.1 phosphoglycerate kinase -
  KCG52_RS06140 (KCG52_06120) yccS 1293050..1295191 (-) 2142 WP_070646400.1 YccS family putative transporter -
  KCG52_RS06145 (KCG52_06125) pilU 1295355..1296584 (-) 1230 WP_254343381.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCG52_RS06150 (KCG52_06130) pilT 1296671..1297717 (-) 1047 WP_070822537.1 type IV pilus twitching motility protein PilT Machinery gene
  KCG52_RS06155 (KCG52_06135) - 1297835..1298533 (+) 699 WP_254343382.1 YggS family pyridoxal phosphate-dependent enzyme -
  KCG52_RS06160 (KCG52_06140) proC 1298610..1299407 (+) 798 WP_254343383.1 pyrroline-5-carboxylate reductase -
  KCG52_RS06165 (KCG52_06145) - 1299410..1299967 (+) 558 WP_254343390.1 YggT family protein -
  KCG52_RS06170 (KCG52_06150) dksA 1300200..1300616 (+) 417 WP_003747644.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38478.10 Da        Isoelectric Point: 7.1311

>NTDB_id=486801 KCG52_RS06150 WP_070822537.1 1296671..1297717(-) (pilT) [Neisseria subflava strain HP0048]
MQITDLLAFGVKNKASDLHLSSGISPMIRVHGDIRRINLPEMSSEEVGTMITSVMNDHQRKLYQQDFEVDFSFELPNVAR
FRVNAFMTERGPAAVFRTIPSTVLTLEELRAPRIFQKIAENPRGLVLVTGPTGSGKSTTLAAMVNYINETQPAHILTIED
PIEFVHQSKKALINQRELHQHTHSFTNALRSALREDPDVILIGEMRDPETISLALTAAETGHLVFGTLHTTGAAKTIDRI
VDVFPAGEKEIVRSMLSESLRAIISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINAALQTGQAHGMQTLDQA
LQTLVRQGTISPEMARSKAQNIENLSIF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=486801 KCG52_RS06150 WP_070822537.1 1296671..1297717(-) (pilT) [Neisseria subflava strain HP0048]
ATGCAGATTACCGACTTACTCGCGTTCGGCGTGAAAAACAAAGCCTCCGACCTCCATTTGAGTTCGGGCATTTCCCCCAT
GATACGCGTACACGGCGACATCCGCCGCATCAACCTGCCCGAGATGAGTTCGGAAGAAGTCGGTACCATGATTACCTCGG
TCATGAACGACCACCAGCGCAAGCTCTACCAACAGGATTTTGAAGTCGATTTCTCATTTGAGCTGCCCAATGTTGCCCGT
TTCCGCGTCAACGCCTTTATGACCGAACGCGGCCCGGCCGCCGTATTCCGTACCATTCCGAGCACCGTCCTGACGCTGGA
AGAATTGCGTGCGCCGCGCATTTTCCAAAAAATTGCCGAAAATCCGCGCGGTTTGGTCTTGGTAACCGGCCCGACCGGTT
CGGGTAAATCCACCACGCTGGCGGCGATGGTCAACTACATCAACGAAACCCAACCGGCCCACATTCTGACCATCGAAGAC
CCGATTGAGTTCGTCCATCAAAGCAAAAAAGCGCTCATCAACCAACGCGAGCTGCATCAGCATACCCACAGCTTCACCAA
CGCCCTGCGTTCCGCCCTGCGCGAAGACCCCGACGTGATTCTGATCGGCGAGATGCGCGACCCTGAAACCATCTCCCTCG
CCCTGACCGCCGCCGAAACCGGCCACTTGGTTTTCGGCACGCTGCACACGACCGGCGCCGCCAAAACCATCGACCGTATT
GTCGACGTATTCCCAGCCGGCGAAAAAGAAATCGTGCGCTCCATGTTGTCCGAATCCTTGCGCGCCATCATTTCCCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGCGTCGCCGCGCACGAAATCCTGATTTCCACACCGGCCGTGCGCAACCTCA
TCCGCGAAAACAAAATCGCCCAAATCAACGCCGCCCTCCAAACCGGTCAGGCCCACGGTATGCAAACCCTCGATCAAGCC
CTGCAAACCCTTGTCCGCCAAGGCACCATTAGCCCCGAGATGGCACGCAGTAAAGCGCAAAACATCGAAAACCTCTCTAT
TTTCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

86.455

99.713

0.862
  pilT Neisseria gonorrhoeae MS11

86.455

99.713

0.862
  pilT Acinetobacter nosocomialis M2

68.696

99.138

0.681
  pilT Acinetobacter baumannii D1279779

68.696

99.138

0.681
  pilT Acinetobacter baumannii strain A118

68.696

99.138

0.681
  pilT Pseudomonas stutzeri DSM 10701

68.116

99.138

0.675
  pilT Pseudomonas aeruginosa PAK

67.826

99.138

0.672
  pilT Acinetobacter baylyi ADP1

67.536

99.138

0.67
  pilT Legionella pneumophila strain Lp02

65.396

97.989

0.641
  pilT Legionella pneumophila strain ERS1305867

65.396

97.989

0.641
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65

97.701

0.635
  pilT Vibrio cholerae strain A1552

65

97.701

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.737

97.414

0.494
  pilU Pseudomonas stutzeri DSM 10701

41.092

100

0.411
  pilU Vibrio cholerae strain A1552

39.655

100

0.397
  pilU Acinetobacter baylyi ADP1

39.08

100

0.391