Detailed information NTG0486800

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KCG52_RS06145
• Genome accession: NZ_CP073114
• Coordinates: 1295355..1296584 (-)
• Length: 409 a.a.
• NCBI ID: WP_254343381.1
• Uniprot ID: -
• Organism: Neisseria subflava strain HP0048
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1290355..1301584

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCG52_RS06135 (KCG52_06115)	-	1291776..1292954 (+)	1179	WP_254343380.1	phosphoglycerate kinase	-
KCG52_RS06140 (KCG52_06120)	yccS	1293050..1295191 (-)	2142	WP_070646400.1	YccS family putative transporter	-
KCG52_RS06145 (KCG52_06125)	pilU	1295355..1296584 (-)	1230	WP_254343381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KCG52_RS06150 (KCG52_06130)	pilT	1296671..1297717 (-)	1047	WP_070822537.1	type IV pilus twitching motility protein PilT	Machinery gene
KCG52_RS06155 (KCG52_06135)	-	1297835..1298533 (+)	699	WP_254343382.1	YggS family pyridoxal phosphate-dependent enzyme	-
KCG52_RS06160 (KCG52_06140)	proC	1298610..1299407 (+)	798	WP_254343383.1	pyrroline-5-carboxylate reductase	-
KCG52_RS06165 (KCG52_06145)	-	1299410..1299967 (+)	558	WP_254343390.1	YggT family protein	-
KCG52_RS06170 (KCG52_06150)	dksA	1300200..1300616 (+)	417	WP_003747644.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 409 a.a.
• Molecular weight: 45891.38 Da
• Isoelectric Point: 6.0120

>NTDB_id=486800 KCG52_RS06145 WP_254343381.1 1295355..1296584(-) (pilU) [Neisseria subflava strain HP0048]
MSDLAPLHDLLSEMVQAYSQKNQAPHIPTPAEIGTHLHPLLDRMCEEAEKRNASDIFISAGFPPAIKVGGTLTPMPHKAL
TGADTAAIAESTMNPDQLEAFNRDWEINYSVQSRSNTRYRVNAYHEQGRVGMVLRRVSQDIPEMKTLGLPEKLQELALAP
RGLLILSGPTGSGKSTTMASMLNYRNQKLPGHIVTIEDPIEFIYKPCRSIFTQREIGIDTDNWKTAVQSAMRQAPDVVCI
GEVRSEESMEYALQLAQTGHLCVFTIHASTASQTIERIINFYPEDRRQQVLMDLALNLVGIIGQRLVVKKNRQRTAVTDL
LLNTPAMQDLIFKGELLEIRDLMARSGSNGMQTFDQNLFNLYAQGQIELSEALRQAFSANDLRLRIQMHDEGNNTERLYD
RISDLNLMT

Nucleotide

• Length: 1230 bp

>NTDB_id=486800 KCG52_RS06145 WP_254343381.1 1295355..1296584(-) (pilU) [Neisseria subflava strain HP0048]
ATGAGCGACCTCGCCCCTCTGCACGACCTGTTAAGCGAAATGGTGCAAGCCTACTCACAAAAAAACCAAGCACCGCACAT
CCCCACCCCGGCCGAAATCGGCACGCATCTTCACCCCCTGCTCGACCGCATGTGTGAAGAGGCCGAAAAACGCAACGCCT
CCGACATCTTCATCAGCGCAGGCTTTCCCCCTGCCATCAAAGTCGGCGGCACCCTCACGCCCATGCCCCACAAAGCGCTG
ACCGGCGCGGACACCGCCGCCATCGCCGAATCCACCATGAACCCCGACCAGCTCGAAGCCTTCAACCGAGACTGGGAAAT
CAACTATTCCGTCCAATCGCGCAGCAACACGCGCTACCGCGTCAACGCCTACCACGAGCAAGGCCGCGTCGGCATGGTAT
TGCGCCGCGTCAGCCAAGACATTCCCGAAATGAAGACCCTAGGTTTGCCCGAAAAGCTCCAAGAGCTTGCACTCGCGCCA
CGCGGCCTGCTGATCCTCTCCGGTCCGACCGGTTCGGGTAAGTCCACCACCATGGCCTCCATGCTCAACTACCGCAACCA
AAAACTGCCCGGCCATATCGTTACCATCGAAGACCCCATCGAGTTTATCTACAAACCTTGCCGCAGCATCTTCACCCAGC
GCGAAATCGGCATCGATACCGACAACTGGAAAACCGCCGTTCAAAGCGCCATGCGTCAAGCGCCCGACGTCGTCTGCATC
GGCGAAGTCCGCAGCGAAGAAAGCATGGAATACGCACTCCAACTGGCTCAAACCGGCCATCTGTGCGTTTTCACCATCCA
TGCCAGCACGGCCTCCCAAACCATCGAGCGCATCATCAACTTCTATCCGGAAGACCGCCGCCAACAGGTTTTGATGGATT
TGGCGTTAAACCTCGTCGGCATCATCGGCCAACGCCTCGTCGTCAAGAAAAACCGCCAACGCACGGCCGTTACAGACCTC
CTGCTCAACACTCCGGCCATGCAGGACTTGATTTTCAAAGGCGAATTGCTGGAAATCCGCGACCTGATGGCACGCTCCGG
CAGCAACGGCATGCAGACGTTCGACCAAAACCTCTTCAACCTCTATGCACAAGGCCAAATCGAACTCAGCGAAGCCCTGC
GCCAAGCGTTTTCCGCCAACGATTTGCGCCTGCGCATTCAAATGCACGACGAGGGCAACAATACCGAGCGTCTCTACGAC
CGCATCAGCGACCTCAACCTGATGACCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (130-308)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	47.887	86.797	0.416
pilU	Pseudomonas stutzeri DSM 10701	47.813	83.863	0.401
pilU	Vibrio cholerae strain A1552	47.181	82.396	0.389