Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IG196_RS27720 Genome accession   NZ_CP062121
Coordinates   5983314..5984357 (+) Length   347 a.a.
NCBI ID   WP_056576373.1    Uniprot ID   A0A9X8CZE2
Organism   Variovorax sp. 38R     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5978314..5989357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG196_RS27695 (IG196_27695) - 5979153..5979848 (-) 696 WP_192193872.1 DUF2242 domain-containing protein -
  IG196_RS27700 (IG196_27700) - 5979961..5981235 (-) 1275 WP_099793335.1 Glu/Leu/Phe/Val family dehydrogenase -
  IG196_RS27705 (IG196_27705) - 5981487..5981942 (+) 456 WP_099793336.1 hypothetical protein -
  IG196_RS27710 (IG196_27710) - 5982086..5982553 (+) 468 WP_062478450.1 Lrp/AsnC ligand binding domain-containing protein -
  IG196_RS27715 (IG196_27715) - 5982561..5983262 (-) 702 WP_192193873.1 YggS family pyridoxal phosphate-dependent enzyme -
  IG196_RS27720 (IG196_27720) pilT 5983314..5984357 (+) 1044 WP_056576373.1 type IV pilus twitching motility protein PilT Machinery gene
  IG196_RS27725 (IG196_27725) pilU 5984387..5985523 (+) 1137 WP_101493812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IG196_RS27730 (IG196_27730) - 5985590..5986498 (+) 909 WP_192193874.1 NAD(P)-dependent oxidoreductase -
  IG196_RS27735 (IG196_27735) - 5986529..5987314 (-) 786 WP_180839091.1 BON domain-containing protein -
  IG196_RS27740 (IG196_27740) - 5987311..5987961 (-) 651 WP_192193875.1 SIS domain-containing protein -
  IG196_RS27745 (IG196_27745) - 5988045..5988416 (-) 372 WP_192193876.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.83 Da        Isoelectric Point: 6.8356

>NTDB_id=486146 IG196_RS27720 WP_056576373.1 5983314..5984357(+) (pilT) [Variovorax sp. 38R]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=486146 IG196_RS27720 WP_056576373.1 5983314..5984357(+) (pilT) [Variovorax sp. 38R]
GTGGACATCACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTGGACCACAAGGCGGTGCATGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCATTACGAAGAATTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTTCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCTGCGGTGTTTCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACTACCTCAACGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGGCCGATGACGCTGTCGTTCTCGAA
CGCACTGCGCTCCGCCCTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAGTTGCGCGACCTCGAAACCATCCGCCTCG
CGATGACCGCGGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAAGAAATGATCCGCGCCATGCTGTCTGAGTCTTTGCAAGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACAGAGCTGGTGCGCCGCAATACGATCTCTGCCGCCGAAGCGCGCGGCAAGGCCAAGATCCCAGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398