Detailed information NTG0486147

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IG196_RS27725
• Genome accession: NZ_CP062121
• Coordinates: 5984387..5985523 (+)
• Length: 378 a.a.
• NCBI ID: WP_101493812.1
• Uniprot ID: -
• Organism: Variovorax sp. 38R
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5979387..5990523

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IG196_RS27700 (IG196_27700)	-	5979961..5981235 (-)	1275	WP_099793335.1	Glu/Leu/Phe/Val family dehydrogenase	-
IG196_RS27705 (IG196_27705)	-	5981487..5981942 (+)	456	WP_099793336.1	hypothetical protein	-
IG196_RS27710 (IG196_27710)	-	5982086..5982553 (+)	468	WP_062478450.1	Lrp/AsnC ligand binding domain-containing protein	-
IG196_RS27715 (IG196_27715)	-	5982561..5983262 (-)	702	WP_192193873.1	YggS family pyridoxal phosphate-dependent enzyme	-
IG196_RS27720 (IG196_27720)	pilT	5983314..5984357 (+)	1044	WP_056576373.1	type IV pilus twitching motility protein PilT	Machinery gene
IG196_RS27725 (IG196_27725)	pilU	5984387..5985523 (+)	1137	WP_101493812.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IG196_RS27730 (IG196_27730)	-	5985590..5986498 (+)	909	WP_192193874.1	NAD(P)-dependent oxidoreductase	-
IG196_RS27735 (IG196_27735)	-	5986529..5987314 (-)	786	WP_180839091.1	BON domain-containing protein	-
IG196_RS27740 (IG196_27740)	-	5987311..5987961 (-)	651	WP_192193875.1	SIS domain-containing protein	-
IG196_RS27745 (IG196_27745)	-	5988045..5988416 (-)	372	WP_192193876.1	YraN family protein	-
IG196_RS27750 (IG196_27750)	rsmI	5988464..5989435 (+)	972	WP_192193877.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
IG196_RS27755 (IG196_27755)	-	5989439..5989654 (-)	216	Protein_5519	glutathione S-transferase family protein	-
IG196_RS27760 (IG196_27760)	-	5989701..5989925 (-)	225	WP_101493816.1	hypothetical protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41782.85 Da
• Isoelectric Point: 6.3136

>NTDB_id=486147 IG196_RS27725 WP_101493812.1 5984387..5985523(+) (pilU) [Variovorax sp. 38R]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMVDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide

• Length: 1137 bp

>NTDB_id=486147 IG196_RS27725 WP_101493812.1 5984387..5985523(+) (pilU) [Variovorax sp. 38R]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTTTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGTGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGGCTGGGCATGCCGCAGGTGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGGTCGATTGGCGCAACGAAAACTCGTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATTCGCG
ACCGCGAAACGATGGAACATGCCGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTGTCGCAACGGCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCGGCGGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCCATCAACTTCGAAGAAGCGATCCGCAACGCCGA
TTCGGCCAACGATCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (41-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.324	92.328	0.585
pilU	Acinetobacter baylyi ADP1	57.263	94.709	0.542
pilU	Vibrio cholerae strain A1552	52.617	96.032	0.505
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.697	89.153	0.407
pilT	Pseudomonas aeruginosa PAK	45.104	89.153	0.402
pilT	Acinetobacter nosocomialis M2	44.144	88.095	0.389
pilT	Acinetobacter baumannii D1279779	44.144	88.095	0.389
pilT	Acinetobacter baumannii strain A118	44.144	88.095	0.389
pilT	Legionella pneumophila strain Lp02	44.377	87.037	0.386
pilT	Legionella pneumophila strain ERS1305867	44.377	87.037	0.386
pilT	Pseudomonas stutzeri DSM 10701	43.323	89.153	0.386
pilT	Acinetobacter baylyi ADP1	43.243	88.095	0.381
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.47	87.831	0.373
pilT	Vibrio cholerae strain A1552	42.47	87.831	0.373