Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IGB07_RS04055 Genome accession   NZ_CP062058
Coordinates   952465..953502 (+) Length   345 a.a.
NCBI ID   WP_055026341.1    Uniprot ID   A0A0Q0NVZ9
Organism   Shewanella sp. WPAGA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 947465..958502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGB07_RS04025 rdgB 948179..948775 (-) 597 WP_055026347.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  IGB07_RS04030 - 948956..949390 (-) 435 WP_055026346.1 DUF4426 domain-containing protein -
  IGB07_RS04035 yggU 949589..949882 (-) 294 WP_192022990.1 DUF167 family protein YggU -
  IGB07_RS04040 - 949987..950535 (-) 549 WP_065109082.1 YggT family protein -
  IGB07_RS04045 proC 950711..951529 (-) 819 WP_119968806.1 pyrroline-5-carboxylate reductase -
  IGB07_RS04050 - 951740..952429 (-) 690 WP_192022362.1 YggS family pyridoxal phosphate-dependent enzyme -
  IGB07_RS04055 pilT 952465..953502 (+) 1038 WP_055026341.1 type IV pilus twitching motility protein PilT Machinery gene
  IGB07_RS04060 pilU 953515..954627 (+) 1113 WP_055026340.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IGB07_RS04065 - 954838..955380 (+) 543 WP_192022363.1 glutathione peroxidase -
  IGB07_RS04070 hemH 955530..956543 (+) 1014 WP_192022364.1 ferrochelatase -
  IGB07_RS04075 ruvX 956679..957107 (-) 429 WP_192022365.1 Holliday junction resolvase RuvX -
  IGB07_RS04080 - 957175..957735 (-) 561 WP_055026336.1 YqgE/AlgH family protein -
  IGB07_RS04085 yciH 957831..958160 (-) 330 WP_065109088.1 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38259.76 Da        Isoelectric Point: 6.6684

>NTDB_id=485921 IGB07_RS04055 WP_055026341.1 952465..953502(+) (pilT) [Shewanella sp. WPAGA9]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQAVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAYNQSRGAGAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINNERHDHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFEAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTVDRI
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLISRDDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=485921 IGB07_RS04055 WP_055026341.1 952465..953502(+) (pilT) [Shewanella sp. WPAGA9]
ATGGAAATAACTGAGTTACTTGCCTTTAGTGTAAAACACAATGCCTCGGATCTACACCTTTCAGCAGGTGTTTCGCCGAT
GATTCGAGTTGATGGTGAAGTCAGAAAGATTAATCTACCTGCCTTAGACCACCAAGCAGTTCATGGTCTGGTGTATGACA
TCATGAATGATAAGCAACGTAAGGACTATGAAGAACATCTAGAAATCGATTTCTCATTTGAAGTGCCTAACTTAGCTCGT
TTTCGTGTGAATGCTTATAACCAATCTCGTGGCGCTGGTGCAGTATTTCGTACCATTCCAAGTGATATTTTATCATTAGA
GCAATTAGGCGCACCTGAAATTTTTAAGAAGATTTCGAGTTTTCCTCGTGGGCTTGTATTGGTTACAGGGCCTACTGGTT
CGGGTAAATCAACCACATTGGCTGCGATGGTAGATTATATTAATAATGAACGTCATGATCACATATTAACCATTGAAGAC
CCAATAGAATTCGTTCACCAAAACAAACAATGTTTGGTCAACCAACGTGAAGTACACAAGCATACCCATAGTTTTGAAGC
GGCGCTTCGAAGTGCACTTCGTGAAGATCCTGATGTCATTCTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCTTAG
CAATGACAGCAGCAGAAACCGGTCATTTAGTATTTGGCACCTTGCATACAACTTCTGCGGCTAAAACCGTTGACCGTATT
GTGGATGTATTCCCTGCGGGTGAGAAAGACATGGTACGTACTATGTTATCTGAATCATTACAGGCGGTAATTTCGCAAAC
CTTGATTAAGAAAGTCGGTGGTGGCCGAGTCGCTGCCCATGAAATTATGATGGGCACACCCGCGATTAGAAACCTAATTC
GTGAAGATAAAGTCGCGCAAATGTACTCAGCTATTCAAACCGGTATGGCGCACGGAATGCAAACACTTGAGCAATGCTTA
CAAAACTTAGTTAACCGTGGCTTAATTAGCCGTGATGATGCGATGTCTAAAAGCTCAAATAAACAAGCTAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0NVZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.134

99.42

0.777
  pilT Acinetobacter nosocomialis M2

78.134

99.42

0.777
  pilT Acinetobacter baumannii strain A118

78.134

99.42

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.681

100

0.777
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Acinetobacter baylyi ADP1

79.697

95.652

0.762
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

68.314

99.71

0.681
  pilT Neisseria gonorrhoeae MS11

68.023

99.71

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.263

98.261

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.964

97.391

0.409
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391