Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IGB07_RS04060 Genome accession   NZ_CP062058
Coordinates   953515..954627 (+) Length   370 a.a.
NCBI ID   WP_055026340.1    Uniprot ID   A0A0Q0J6J6
Organism   Shewanella sp. WPAGA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948515..959627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGB07_RS04030 - 948956..949390 (-) 435 WP_055026346.1 DUF4426 domain-containing protein -
  IGB07_RS04035 yggU 949589..949882 (-) 294 WP_192022990.1 DUF167 family protein YggU -
  IGB07_RS04040 - 949987..950535 (-) 549 WP_065109082.1 YggT family protein -
  IGB07_RS04045 proC 950711..951529 (-) 819 WP_119968806.1 pyrroline-5-carboxylate reductase -
  IGB07_RS04050 - 951740..952429 (-) 690 WP_192022362.1 YggS family pyridoxal phosphate-dependent enzyme -
  IGB07_RS04055 pilT 952465..953502 (+) 1038 WP_055026341.1 type IV pilus twitching motility protein PilT Machinery gene
  IGB07_RS04060 pilU 953515..954627 (+) 1113 WP_055026340.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IGB07_RS04065 - 954838..955380 (+) 543 WP_192022363.1 glutathione peroxidase -
  IGB07_RS04070 hemH 955530..956543 (+) 1014 WP_192022364.1 ferrochelatase -
  IGB07_RS04075 ruvX 956679..957107 (-) 429 WP_192022365.1 Holliday junction resolvase RuvX -
  IGB07_RS04080 - 957175..957735 (-) 561 WP_055026336.1 YqgE/AlgH family protein -
  IGB07_RS04085 yciH 957831..958160 (-) 330 WP_065109088.1 stress response translation initiation inhibitor YciH -
  IGB07_RS04090 - 958445..959005 (+) 561 WP_119968801.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41296.24 Da        Isoelectric Point: 5.9381

>NTDB_id=485922 IGB07_RS04060 WP_055026340.1 953515..954627(+) (pilU) [Shewanella sp. WPAGA9]
MDVRPFLKVMVDRKASDLFVTAGFPPSAKIDGELRPLSESSFTPEQSLDFVESLMTDVQKQEFHESRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHNQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKETMSKSREQGMQTFDQA
LLDLYLEKEISYADALHHADSPNDLRLMIKLQNNESSSSGIMDGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=485922 IGB07_RS04060 WP_055026340.1 953515..954627(+) (pilU) [Shewanella sp. WPAGA9]
ATGGATGTTCGTCCTTTTTTAAAGGTCATGGTAGACCGTAAAGCATCAGATTTATTTGTTACCGCGGGATTTCCGCCGAG
TGCAAAAATTGATGGTGAATTAAGACCGTTATCAGAAAGCAGTTTTACGCCTGAGCAGTCGTTAGATTTCGTAGAGTCTT
TAATGACTGATGTGCAAAAACAAGAGTTTCATGAATCTCGCGAATGTAACTTTGCTTTTGCAGCTAAAGAATTAGGCCGT
TTTCGTGTTAGTGCTTTTTGGCAACGTGAATCACCTGGTTGTGTGATGCGCCGTATTGAAACAAAAATCCCAGAAGTTGA
AGATTTAAAGCTACCGCCCATTTTAAAAGATTTGGTCATGAGTAAACGTGGTCTGATTATTATGGTCGGTGGTACCGGTA
CTGGTAAATCAACCTCGTTGGCTTCGCTTGTTGGTTATCGTAATTCACATGCTCGAGGTCATATTTTAACGATTGAAGAC
CCAGTGGAATTTGTGCATGACCATCGCAAAAGTATTATTACTCAGCGAGAAGTCGGTATAGACACTGACTCTTTTGATGC
TGCGCTTAAAAGCTCACTTCGTCAGGCACCAGATGTTATTTTGATTGGTGAAATTCGAACTCAAGAAACCATGGAGTTTG
CGCTGTCATTTGCTGAAACGGGTCACTTATGTATGGCAACACTCCATGCTAACAATGCTAACCAAGCGTTAGACCGTATT
ATGCATTTAGTGCCTGAAAATAAGCATAACCAACTCTTGTTCGATTTGTCGCTAAACTTACGTGGCATTGTCGCTCAACA
GCTTGTGCCGAAATCAGATGGCTCTGGGCGTCGCGCTGCTATTGAAGTGTTAATCAACACTCCTCGTGTGGCGAGTTTGA
TTGCAAAAAATGAGCTGCATTTACTCAAAGAAACCATGAGTAAGTCACGAGAGCAGGGCATGCAGACTTTTGACCAAGCG
TTATTGGATTTATACTTGGAAAAAGAAATCAGCTATGCAGATGCGCTGCACCATGCTGATTCTCCAAACGATTTACGCCT
AATGATAAAACTGCAAAATAATGAGAGTAGCAGTTCTGGCATTATGGATGGTGTCACGTTAGACATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0J6J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

56.486

100

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.947

86.216

0.37
  pilT Vibrio cholerae strain A1552

42.947

86.216

0.37
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362