Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IEJ03_RS00965 Genome accession   NZ_CP062005
Coordinates   203397..204425 (+) Length   342 a.a.
NCBI ID   WP_192035904.1    Uniprot ID   -
Organism   Halomonas sp. YLGW01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 198397..209425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEJ03_RS00935 - 198654..199133 (-) 480 WP_192035898.1 DUF4426 domain-containing protein -
  IEJ03_RS00940 metW 199169..199771 (-) 603 WP_192035899.1 methionine biosynthesis protein MetW -
  IEJ03_RS00945 - 199771..200922 (-) 1152 WP_192035900.1 homoserine O-acetyltransferase -
  IEJ03_RS00950 - 201010..201600 (-) 591 WP_192035901.1 YggT family protein -
  IEJ03_RS00955 proC 201748..202578 (-) 831 WP_192035902.1 pyrroline-5-carboxylate reductase -
  IEJ03_RS00960 - 202649..203359 (-) 711 WP_192035903.1 YggS family pyridoxal phosphate-dependent enzyme -
  IEJ03_RS00965 pilT 203397..204425 (+) 1029 WP_192035904.1 type IV pilus twitching motility protein PilT Machinery gene
  IEJ03_RS00970 pilU 204476..205612 (+) 1137 WP_192035905.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IEJ03_RS00975 - 205734..206891 (-) 1158 WP_192035906.1 anhydro-N-acetylmuramic acid kinase -
  IEJ03_RS00980 tyrS 207028..208227 (+) 1200 WP_192035907.1 tyrosine--tRNA ligase -
  IEJ03_RS00985 - 208339..208584 (-) 246 WP_192035908.1 hypothetical protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37481.89 Da        Isoelectric Point: 6.4224

>NTDB_id=485893 IEJ03_RS00965 WP_192035904.1 203397..204425(+) (pilT) [Halomonas sp. YLGW01]
MDITELLAFSAKQNASDLHISAGLPPMIRVDGDIRRLNVPAMDDREVRALIYDIMGDKQRRDFEEFLETDFSFEVPGVSR
FRVNAFHQGRGAAAVMRTVPSEVLTMEALGLGEVFRRMASLPRGLVLVTGPTGSGKSTTLAAMIDYINDTRYDHILTIED
PVEFVHRSKRSLINQREVHRDTQGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPGSEKDMVRSMLSESLQGVISQALLKRHGGGRVAAHEILVTTSAIRNLIREDKVAQIYSAIQTGGNLGMQTLDASL
ARLVSDGVVVREEAQAKAKGAI

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=485893 IEJ03_RS00965 WP_192035904.1 203397..204425(+) (pilT) [Halomonas sp. YLGW01]
ATGGATATTACCGAATTGCTGGCATTCTCGGCAAAGCAGAACGCCTCGGACCTGCATATTTCCGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGGGACATTCGCCGGCTCAATGTGCCGGCCATGGACGATCGTGAGGTGCGCGCGCTGATCTACGACA
TCATGGGCGACAAGCAGCGCCGCGACTTCGAGGAGTTCCTGGAGACGGACTTCTCCTTTGAGGTGCCGGGCGTGTCGCGC
TTTCGTGTCAACGCCTTCCATCAGGGGCGCGGGGCGGCCGCGGTGATGCGTACCGTGCCCTCCGAGGTGCTGACCATGGA
GGCGCTGGGCCTGGGCGAGGTCTTCCGCCGCATGGCCTCGTTGCCCCGTGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGTACCACCCTGGCCGCGATGATCGACTACATCAACGACACCCGTTATGACCATATCCTCACCATCGAGGAC
CCGGTGGAGTTCGTGCACCGCAGCAAGCGTTCCCTGATCAACCAGCGCGAGGTGCATCGTGATACCCAGGGCTTCGCCCC
GGCGCTGCGCAGCGCCCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAGCTCCGTGACCTGGAGACCATCCGCCTGG
CCCTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCCAAGACCGTCGACCGCATC
ATCGATGTCTTCCCCGGGTCGGAGAAGGACATGGTGCGTTCGATGCTCTCGGAGTCGCTGCAGGGGGTGATCTCCCAGGC
GCTGCTCAAGCGTCATGGCGGAGGACGGGTGGCGGCGCACGAGATCCTGGTGACGACCTCGGCGATTCGCAACCTTATCC
GGGAAGACAAGGTGGCGCAGATCTACTCGGCGATTCAGACCGGGGGCAACCTGGGCATGCAGACCCTGGATGCGTCGCTG
GCGAGGCTGGTCAGCGATGGGGTCGTGGTGCGCGAGGAAGCTCAGGCCAAGGCCAAGGGCGCGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.761

99.123

0.781
  pilT Pseudomonas aeruginosa PAK

78.761

99.123

0.781
  pilT Acinetobacter nosocomialis M2

75.811

99.123

0.751
  pilT Acinetobacter baumannii D1279779

75.516

99.123

0.749
  pilT Acinetobacter baumannii strain A118

75.516

99.123

0.749
  pilT Acinetobacter baylyi ADP1

75.221

99.123

0.746
  pilT Legionella pneumophila strain Lp02

69.822

98.83

0.69
  pilT Legionella pneumophila strain ERS1305867

69.822

98.83

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

98.538

0.675
  pilT Vibrio cholerae strain A1552

68.546

98.538

0.675
  pilT Neisseria meningitidis 8013

66.082

100

0.661
  pilT Neisseria gonorrhoeae MS11

65.789

100

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.704

94.737

0.509
  pilU Vibrio cholerae strain A1552

40.643

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.953

0.398
  pilU Acinetobacter baylyi ADP1

40.308

95.029

0.383