Detailed information NTG0485894

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IEJ03_RS00970
• Genome accession: NZ_CP062005
• Coordinates: 204476..205612 (+)
• Length: 378 a.a.
• NCBI ID: WP_192035905.1
• Uniprot ID: -
• Organism: Halomonas sp. YLGW01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 199476..210612

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IEJ03_RS00945	-	199771..200922 (-)	1152	WP_192035900.1	homoserine O-acetyltransferase	-
IEJ03_RS00950	-	201010..201600 (-)	591	WP_192035901.1	YggT family protein	-
IEJ03_RS00955	proC	201748..202578 (-)	831	WP_192035902.1	pyrroline-5-carboxylate reductase	-
IEJ03_RS00960	-	202649..203359 (-)	711	WP_192035903.1	YggS family pyridoxal phosphate-dependent enzyme	-
IEJ03_RS00965	pilT	203397..204425 (+)	1029	WP_192035904.1	type IV pilus twitching motility protein PilT	Machinery gene
IEJ03_RS00970	pilU	204476..205612 (+)	1137	WP_192035905.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IEJ03_RS00975	-	205734..206891 (-)	1158	WP_192035906.1	anhydro-N-acetylmuramic acid kinase	-
IEJ03_RS00980	tyrS	207028..208227 (+)	1200	WP_192035907.1	tyrosine--tRNA ligase	-
IEJ03_RS00985	-	208339..208584 (-)	246	WP_192035908.1	hypothetical protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41881.08 Da
• Isoelectric Point: 6.4216

>NTDB_id=485894 IEJ03_RS00970 WP_192035905.1 204476..205612(+) (pilU) [Halomonas sp. YLGW01]
MKANEWLQQLLEIMIEKQASDLLISVNAPPSLKTAGQLTPLGEQVLSPAQVAELVKAALPPAQRERFAAEQEANFALSLP
GRGRFRVSAFQQRNQPAMVLRRISFEIPRFETLNLPPSLAELANAKRGLVLVVGGTGTGKSTTLASMIQQRNETQCGHII
SIEDPIEYVHPHKKSIINQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRKTMEHALTFAETGHLCLATLHANNANQA
LDRIINFFPAERHDQVWLDLSLNLKAIVAQQLLPTVDRSRRAAIEILLRSPLISDLIRKGDVSEIKNIMARSRDMGMQTF
DQALFDLYKEGVISEDIAMVHADSANDLRMMIKFADEGGVADAQGAGKHFELRSGDDF

Nucleotide

• Length: 1137 bp

>NTDB_id=485894 IEJ03_RS00970 WP_192035905.1 204476..205612(+) (pilU) [Halomonas sp. YLGW01]
ATGAAAGCGAACGAATGGTTGCAGCAGTTGCTCGAGATCATGATCGAGAAGCAAGCCTCGGATCTGCTGATATCGGTAAA
TGCCCCGCCGTCTCTCAAGACGGCCGGCCAGCTCACGCCGCTGGGGGAGCAGGTGCTCTCGCCGGCCCAGGTCGCCGAGC
TGGTCAAGGCCGCCCTGCCGCCGGCCCAGCGCGAGCGCTTCGCGGCCGAGCAGGAGGCCAATTTCGCGCTGAGCCTGCCC
GGGCGGGGGCGTTTTCGTGTCAGCGCTTTCCAGCAGCGTAACCAGCCGGCCATGGTGTTGCGGCGGATCTCCTTCGAGAT
TCCGCGCTTCGAGACCCTGAACCTGCCGCCGAGCCTGGCCGAGCTGGCCAATGCAAAGCGCGGCCTGGTGCTGGTGGTCG
GGGGCACCGGCACCGGCAAGTCGACGACCCTGGCCTCGATGATCCAGCAGCGCAACGAGACCCAGTGCGGTCACATCATC
AGCATCGAGGATCCGATCGAGTACGTGCATCCGCACAAGAAGTCGATCATCAATCAGCGGGAAGTCGGCATCGACACCGA
GTCCTTCGAGGTGGCGCTGAAGAATACCCTGCGCCAGGCTCCGGACGTGATCCTGATCGGCGAGATCCGCAGCCGCAAGA
CCATGGAGCATGCGCTGACCTTCGCCGAGACCGGGCATTTGTGCCTGGCGACCCTGCATGCCAACAACGCCAACCAGGCG
CTGGATCGGATCATCAACTTCTTTCCCGCTGAGCGACATGACCAGGTATGGCTCGACCTGTCGTTGAACCTGAAGGCGAT
CGTCGCCCAGCAACTGCTGCCGACGGTGGATCGCAGCCGTCGGGCGGCGATCGAGATCCTGCTGCGCTCGCCGCTGATCA
GCGACCTGATCCGCAAGGGCGATGTCAGCGAGATCAAGAACATCATGGCGCGCTCCCGGGACATGGGCATGCAGACCTTC
GATCAGGCGCTCTTCGATCTGTACAAGGAGGGCGTGATCAGCGAGGACATCGCCATGGTGCATGCCGACTCGGCCAACGA
TCTGCGCATGATGATCAAGTTTGCCGATGAAGGCGGCGTGGCCGACGCCCAGGGCGCCGGCAAGCACTTCGAGCTGCGAA
GCGGCGATGATTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (71-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.484	98.413	0.595
pilU	Acinetobacter baylyi ADP1	58.807	93.122	0.548
pilU	Vibrio cholerae strain A1552	58.333	92.063	0.537
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.857	90.741	0.389
pilT	Vibrio cholerae strain A1552	43.119	86.508	0.373
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	43.119	86.508	0.373
pilT	Neisseria meningitidis 8013	40.525	90.741	0.368
pilT	Neisseria gonorrhoeae MS11	40.525	90.741	0.368
pilT	Legionella pneumophila strain Lp02	41.265	87.831	0.362
pilT	Legionella pneumophila strain ERS1305867	41.265	87.831	0.362