Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IEZ30_RS22790 Genome accession   NZ_CP061849
Coordinates   4905381..4906418 (-) Length   345 a.a.
NCBI ID   WP_043589321.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain Bb2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4900381..4911418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEZ30_RS22770 (IEZ30_22770) - 4900485..4901192 (-) 708 WP_019100948.1 16S rRNA pseudouridine(516) synthase -
  IEZ30_RS22775 (IEZ30_22775) - 4901234..4903150 (-) 1917 WP_161524452.1 diguanylate cyclase -
  IEZ30_RS22780 (IEZ30_22780) - 4903480..4904067 (+) 588 WP_043589326.1 TetR/AcrR family transcriptional regulator -
  IEZ30_RS22785 (IEZ30_22785) pilU 4904199..4905335 (-) 1137 WP_043589323.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IEZ30_RS22790 (IEZ30_22790) pilT 4905381..4906418 (-) 1038 WP_043589321.1 type IV pilus twitching motility protein PilT Machinery gene
  IEZ30_RS22795 (IEZ30_22795) - 4906694..4907386 (+) 693 WP_118268480.1 YggS family pyridoxal phosphate-dependent enzyme -
  IEZ30_RS22800 (IEZ30_22800) proC 4907440..4908243 (+) 804 WP_118268481.1 pyrroline-5-carboxylate reductase -
  IEZ30_RS22805 (IEZ30_22805) - 4908252..4908833 (+) 582 WP_019102524.1 YggT family protein -
  IEZ30_RS22810 (IEZ30_22810) - 4908925..4909230 (+) 306 WP_019102525.1 c-type cytochrome -
  IEZ30_RS22815 (IEZ30_22815) dksA 4909515..4909925 (+) 411 WP_019102526.1 RNA polymerase-binding protein DksA -
  IEZ30_RS22820 (IEZ30_22820) aroG 4910157..4911218 (+) 1062 WP_019102527.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38336.12 Da        Isoelectric Point: 6.7774

>NTDB_id=485138 IEZ30_RS22790 WP_043589321.1 4905381..4906418(-) (pilT) [Chromobacterium haemolyticum strain Bb2]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDTFECDFSFDLPGIAR
FRVNSFVQNRGMGAVFRVIPSKVLSLEQLGAPKIFQEIAAFPRGLVLVTGPTGSGKSTTLAAMIDFINDNHFSHILTVED
PIEFVHDSKKCLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGSGRVAAHEIMLGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LQELVRRNMVSPADARQKAANKDQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=485138 IEZ30_RS22790 WP_043589321.1 4905381..4906418(-) (pilT) [Chromobacterium haemolyticum strain Bb2]
ATGGAAATTTCGGAGCTCTTGGCCTTTACGGTCAAGAACAAGGCGTCCGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGGGTGAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGATTACCAGCGCAAAATCTTCGAAGACACCTTCGAGTGCGACTTTTCTTTCGATCTGCCCGGCATCGCCCGT
TTCCGCGTCAACTCCTTCGTGCAGAACCGCGGCATGGGCGCGGTGTTTCGGGTGATTCCGTCCAAGGTGTTGTCGCTGGA
ACAGTTGGGCGCGCCCAAGATATTCCAGGAAATCGCCGCTTTTCCGCGCGGGCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAGTCCACTACGCTGGCGGCGATGATAGATTTCATCAACGACAACCACTTCTCCCACATCCTGACTGTCGAGGAT
CCGATCGAGTTCGTCCACGACAGCAAGAAGTGCCTGATCAACCAGCGTGAACTGGGTTTGCAGACACACAGCTTCGCCAA
TGCCTTGAAGTCCGCCTTGCGGGAAGACCCGGATGTGATCCTGGTGGGCGAACTGCGCGATCTGGAAACCATACGCTTGG
CGCTGACCGCGGCGGAAACCGGTCACTTGGTGTTCGGCACGCTGCACACCAGTTCCGCGGCCAAGACCATAGACCGCATC
GTCGATGTGTTCCCGGCAGGGGAAAAGGAGATGGTGCGCTCCATGCTGTCCGAGTCGGTGCGCGCGGTGATCGCCCAGAC
CCTGCTCAAAACCAAGGACGGCAGCGGACGGGTGGCGGCGCATGAAATCATGCTGGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATTGCTCAAATCAACTCCATGATCCAGACCGGCCAGCAGCATGGGATGCAGACGCTGGACCAGTGC
CTGCAGGAGCTGGTGCGGCGCAATATGGTGTCGCCGGCGGACGCCAGGCAAAAGGCCGCCAACAAGGATCAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

100

0.722
  pilT Acinetobacter baumannii strain A118

72.174

100

0.722
  pilT Acinetobacter baumannii D1279779

72.174

100

0.722
  pilT Neisseria meningitidis 8013

71.429

99.42

0.71
  pilT Neisseria gonorrhoeae MS11

71.429

99.42

0.71
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

70.725

100

0.707
  pilT Pseudomonas aeruginosa PAK

70.725

100

0.707
  pilT Legionella pneumophila strain ERS1305867

70.725

100

0.707
  pilT Acinetobacter baylyi ADP1

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.971

0.652
  pilT Vibrio cholerae strain A1552

66.568

97.971

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.888

98.261

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406