Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IEZ30_RS22785 Genome accession   NZ_CP061849
Coordinates   4904199..4905335 (-) Length   378 a.a.
NCBI ID   WP_043589323.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain Bb2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4899199..4910335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEZ30_RS22765 (IEZ30_22765) - 4899937..4900326 (-) 390 WP_043589328.1 VOC family protein -
  IEZ30_RS22770 (IEZ30_22770) - 4900485..4901192 (-) 708 WP_019100948.1 16S rRNA pseudouridine(516) synthase -
  IEZ30_RS22775 (IEZ30_22775) - 4901234..4903150 (-) 1917 WP_161524452.1 diguanylate cyclase -
  IEZ30_RS22780 (IEZ30_22780) - 4903480..4904067 (+) 588 WP_043589326.1 TetR/AcrR family transcriptional regulator -
  IEZ30_RS22785 (IEZ30_22785) pilU 4904199..4905335 (-) 1137 WP_043589323.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IEZ30_RS22790 (IEZ30_22790) pilT 4905381..4906418 (-) 1038 WP_043589321.1 type IV pilus twitching motility protein PilT Machinery gene
  IEZ30_RS22795 (IEZ30_22795) - 4906694..4907386 (+) 693 WP_118268480.1 YggS family pyridoxal phosphate-dependent enzyme -
  IEZ30_RS22800 (IEZ30_22800) proC 4907440..4908243 (+) 804 WP_118268481.1 pyrroline-5-carboxylate reductase -
  IEZ30_RS22805 (IEZ30_22805) - 4908252..4908833 (+) 582 WP_019102524.1 YggT family protein -
  IEZ30_RS22810 (IEZ30_22810) - 4908925..4909230 (+) 306 WP_019102525.1 c-type cytochrome -
  IEZ30_RS22815 (IEZ30_22815) dksA 4909515..4909925 (+) 411 WP_019102526.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42326.44 Da        Isoelectric Point: 6.2382

>NTDB_id=485137 IEZ30_RS22785 WP_043589323.1 4904199..4905335(-) (pilU) [Chromobacterium haemolyticum strain Bb2]
MEKDQASKFILDLLKHATGKNASDVFIAADFPPAMKIDGKITPVAPQPLSAQHTKELVRAVMNDRQTEEFETKKEANFAI
NPPGLGRFRVSAYVQQGHAGMVLRKINTEIPTFDQLNLPLVLRDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSHNHD
HIITIEDPIEYVHQHKKSIITQREVGVDTDDWDIALKNTLRQAPDVILMGEIRDRETMGYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLVPIKSGRGRVAAVEILLNSPLVSDMIFKGEIASLKEVMGRSRETG
MQTFDQSLFELYEADLISYEDALRNADSINDLRLKIKLYSEGSKSRDPLEGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=485137 IEZ30_RS22785 WP_043589323.1 4904199..4905335(-) (pilU) [Chromobacterium haemolyticum strain Bb2]
ATGGAAAAAGACCAGGCTTCCAAGTTCATACTGGATCTGCTGAAGCACGCCACCGGCAAGAACGCCTCCGACGTTTTCAT
CGCCGCCGATTTTCCGCCGGCGATGAAGATAGACGGCAAGATCACCCCGGTGGCGCCGCAGCCCCTGTCCGCCCAGCACA
CCAAGGAACTGGTGCGCGCGGTGATGAACGACCGGCAGACCGAGGAGTTCGAAACCAAGAAGGAGGCGAACTTCGCGATC
AACCCGCCGGGTCTGGGACGTTTCCGCGTCAGCGCCTATGTGCAGCAGGGCCATGCCGGCATGGTGTTGCGCAAGATAAA
CACTGAAATCCCCACCTTCGACCAGCTCAACCTGCCGCTGGTGCTGCGCGACATCGCGCTGATCAAGCGCGGCCTGGTGA
TCTTCGTCGGAGGCACCGGTTCAGGCAAATCCACCTCGCTGGCCGCGCTGGTGGACTGGCGCAATAGCCACAACCACGAC
CACATCATCACCATCGAAGATCCGATCGAATACGTGCATCAGCACAAGAAGTCCATCATCACCCAGCGCGAGGTGGGCGT
GGACACCGATGATTGGGACATCGCCTTGAAGAACACCTTGCGTCAAGCGCCGGACGTGATTCTGATGGGCGAGATTCGCG
ATCGCGAAACCATGGGTTATGGGCTGCAGTTCGCCGAAACCGGCCATTTGTGCCTGGCCACGCTGCATGCCAACAACGCC
AACCAGGCGCTGGACCGCATTCTCAACTTCTTCCCCGAAGAACGCCATCAGCAGGTGTTGATGGATCTGTCGCTGAATAT
GCGCTCCATCATCTCTCAGCGGCTGGTGCCGATCAAAAGCGGCCGCGGCCGGGTGGCGGCGGTGGAAATTCTGCTCAACA
GCCCGTTGGTGTCCGACATGATCTTCAAAGGCGAAATCGCCAGTCTGAAGGAGGTGATGGGCCGCTCGCGCGAGACCGGC
ATGCAGACCTTCGACCAGTCCTTGTTCGAGTTGTACGAAGCGGACCTGATCAGCTACGAGGACGCCCTGCGCAACGCCGA
CTCCATTAACGACCTGAGGCTGAAGATCAAGCTTTACAGCGAGGGTTCCAAGTCGCGCGACCCGCTGGAAGGCATAGACC
ACCTAGATATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.032

92.328

0.563
  pilU Acinetobacter baylyi ADP1

55.738

96.825

0.54
  pilU Vibrio cholerae strain A1552

53.602

91.799

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Acinetobacter baylyi ADP1

41.003

89.683

0.368
  pilT Pseudomonas aeruginosa PAK

40.653

89.153

0.362