Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H2514_RS09880 Genome accession   NZ_CP061847
Coordinates   2236534..2237571 (-) Length   345 a.a.
NCBI ID   WP_036194074.1    Uniprot ID   A0A0A0EM50
Organism   Lysobacter sp. CW239     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2231534..2242571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS09850 (H2514_09850) - 2232096..2233031 (-) 936 WP_036194069.1 aspartate carbamoyltransferase catalytic subunit -
  H2514_RS09855 (H2514_09855) ruvX 2233048..2233599 (-) 552 WP_052103786.1 Holliday junction resolvase RuvX -
  H2514_RS09860 (H2514_09860) - 2233596..2234162 (-) 567 WP_036194070.1 YqgE/AlgH family protein -
  H2514_RS09865 (H2514_09865) - 2234386..2234943 (+) 558 WP_036194071.1 DNA-3-methyladenine glycosylase I -
  H2514_RS09870 (H2514_09870) - 2234922..2235206 (+) 285 WP_224743862.1 DUF4377 domain-containing protein -
  H2514_RS09875 (H2514_09875) pilU 2235327..2236466 (-) 1140 WP_036194073.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H2514_RS09880 (H2514_09880) pilT 2236534..2237571 (-) 1038 WP_036194074.1 type IV pilus twitching motility protein PilT Machinery gene
  H2514_RS09885 (H2514_09885) - 2237745..2238413 (+) 669 WP_255516806.1 YggS family pyridoxal phosphate-dependent enzyme -
  H2514_RS09890 (H2514_09890) proC 2238498..2239343 (+) 846 WP_036194076.1 pyrroline-5-carboxylate reductase -
  H2514_RS09895 (H2514_09895) - 2239402..2239881 (+) 480 WP_036194077.1 DUF4426 domain-containing protein -
  H2514_RS09900 (H2514_09900) - 2240225..2241055 (-) 831 WP_052103788.1 ABC transporter substrate-binding protein -
  H2514_RS09905 (H2514_09905) pdxH 2241052..2241645 (-) 594 WP_156969364.1 pyridoxamine 5'-phosphate oxidase -
  H2514_RS09910 (H2514_09910) - 2241812..2242015 (-) 204 WP_036194079.1 dodecin family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38476.19 Da        Isoelectric Point: 6.7298

>NTDB_id=485085 H2514_RS09880 WP_036194074.1 2236534..2237571(-) (pilT) [Lysobacter sp. CW239]
MDIAELLAFSVKNKASDLHLSAGMPPMIRVDGDVRRINIPALDHKTVHALVYDIMSDKQRRDFEEFLETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGTPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQNAGMMTLDQHL
QDLVKRGLILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=485085 H2514_RS09880 WP_036194074.1 2236534..2237571(-) (pilT) [Lysobacter sp. CW239]
ATGGATATCGCCGAACTATTGGCATTCTCGGTAAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCATGCCGCCGAT
GATCCGGGTCGACGGGGACGTGCGCCGGATCAACATCCCGGCGCTGGACCACAAGACCGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAACGCCGCGACTTCGAGGAATTCCTCGAGACCGACTTCTCGTTCGAGATCCCCGGCCTGGCGCGG
TTCCGCGTCAACGCGTTCAACCAGAATCGTGGCGCGGGCGCGGTGTTCCGCACGATTCCGTCCGAGGTGCTGACCCTGGA
AGACCTGGGCACGCCGCGGATCTTCAAGGAGCTGATCGACCAGCCGCAGGGCTTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTGTCGGTGGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGTGAGGACCCGGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACGATCCGGCTGG
CGCTGACCGCCGCGGAAACCGGGCATCTGGTGTTCGGCACGCTGCATACCAGTTCGGCGGCCAAGACCGTGGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGACGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGAACGCCGGGATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTGAAGCGCGGCCTGATCCTGCGCCCGCAGGCCCGCGAGTACGCCAAGGACAAACGCCTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0EM50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.523

99.71

0.803
  pilT Acinetobacter baumannii strain A118

80.523

99.71

0.803
  pilT Acinetobacter nosocomialis M2

80.523

99.71

0.803
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

41.818

95.652

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383