Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H2514_RS09875 Genome accession   NZ_CP061847
Coordinates   2235327..2236466 (-) Length   379 a.a.
NCBI ID   WP_036194073.1    Uniprot ID   -
Organism   Lysobacter sp. CW239     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2230327..2241466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS09845 (H2514_09845) - 2230915..2232075 (+) 1161 WP_224743861.1 DNA ligase -
  H2514_RS09850 (H2514_09850) - 2232096..2233031 (-) 936 WP_036194069.1 aspartate carbamoyltransferase catalytic subunit -
  H2514_RS09855 (H2514_09855) ruvX 2233048..2233599 (-) 552 WP_052103786.1 Holliday junction resolvase RuvX -
  H2514_RS09860 (H2514_09860) - 2233596..2234162 (-) 567 WP_036194070.1 YqgE/AlgH family protein -
  H2514_RS09865 (H2514_09865) - 2234386..2234943 (+) 558 WP_036194071.1 DNA-3-methyladenine glycosylase I -
  H2514_RS09870 (H2514_09870) - 2234922..2235206 (+) 285 WP_224743862.1 DUF4377 domain-containing protein -
  H2514_RS09875 (H2514_09875) pilU 2235327..2236466 (-) 1140 WP_036194073.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H2514_RS09880 (H2514_09880) pilT 2236534..2237571 (-) 1038 WP_036194074.1 type IV pilus twitching motility protein PilT Machinery gene
  H2514_RS09885 (H2514_09885) - 2237745..2238413 (+) 669 WP_255516806.1 YggS family pyridoxal phosphate-dependent enzyme -
  H2514_RS09890 (H2514_09890) proC 2238498..2239343 (+) 846 WP_036194076.1 pyrroline-5-carboxylate reductase -
  H2514_RS09895 (H2514_09895) - 2239402..2239881 (+) 480 WP_036194077.1 DUF4426 domain-containing protein -
  H2514_RS09900 (H2514_09900) - 2240225..2241055 (-) 831 WP_052103788.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42268.64 Da        Isoelectric Point: 6.4191

>NTDB_id=485084 H2514_RS09875 WP_036194073.1 2235327..2236466(-) (pilU) [Lysobacter sp. CW239]
MSTAESIDFTSFLKLMSHQKASDLFITAGMPPSMKVNGTLQPITQNPLTPQQSRDLVLNVMNPMQREEFEKTHEANFAIG
LAGVGRFRVSCFYQRNQVGMVLRRIESKIPTIDELNLPPVLKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGH
IITIEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNAN
QAMDRMINFFPEDRRNQLLMDLSLNLKGIIAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGM
RTFDQALFELYQAGEITYEDALRYADSQNEVRLRIKLAQGGDAKTLSAGMDGIEVAEAR

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=485084 H2514_RS09875 WP_036194073.1 2235327..2236466(-) (pilU) [Lysobacter sp. CW239]
ATGAGCACCGCAGAAAGCATCGACTTCACCTCGTTCCTCAAGCTGATGTCGCACCAGAAGGCTTCCGACCTGTTCATCAC
CGCCGGCATGCCGCCGTCGATGAAGGTCAACGGCACCCTGCAGCCGATCACGCAGAACCCGCTCACGCCGCAGCAGAGCC
GTGACCTGGTACTCAACGTGATGAACCCGATGCAGCGCGAGGAGTTCGAGAAGACCCACGAGGCCAACTTCGCGATCGGC
CTGGCCGGGGTCGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTTGGCATGGTGCTGCGCCGGATCGAGAG
CAAGATCCCGACCATCGACGAGCTCAACCTGCCGCCGGTGCTGAAGACCCTGGCGATGACCAAGCGCGGCATCATCCTGT
TCGTCGGCGCGACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCAC
ATCATCACCATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGA
CACCGACAGCTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCC
GCGAGGGCATGGACCACGCGATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACACTGCACGCCAACAACGCCAAC
CAGGCGATGGACCGGATGATCAACTTCTTCCCGGAGGACCGGCGCAACCAGTTGCTGATGGACCTGTCGCTGAACCTCAA
GGGGATCATCGCCCAGCAACTGATTCCGACCCCGGACGGCAAGGGCCGCCGGGTGGCGATGGAGATCCTGCTCGGCACGC
CATTGGTGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCGGTGCAGTTGGGCATG
CGCACCTTCGACCAGGCGTTGTTCGAGCTGTACCAGGCCGGCGAGATCACCTACGAGGACGCGCTGCGTTATGCCGACTC
GCAGAACGAGGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGCGACGCGAAGACGCTGTCGGCCGGCATGGACGGCATCG
AAGTGGCCGAGGCGCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

96.834

0.66
  pilU Acinetobacter baylyi ADP1

63.842

93.404

0.596
  pilU Vibrio cholerae strain A1552

53.933

93.931

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.823

98.417

0.412
  pilT Pseudomonas aeruginosa PAK

40.896

88.391

0.361
  pilT Acinetobacter nosocomialis M2

39.826

90.765

0.361
  pilT Acinetobacter baumannii D1279779

39.826

90.765

0.361
  pilT Acinetobacter baumannii strain A118

39.826

90.765

0.361