Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IEE84_RS01595 Genome accession   NZ_CP061739
Coordinates   369196..370251 (-) Length   351 a.a.
NCBI ID   WP_179798169.1    Uniprot ID   -
Organism   Psychrobacter sp. 28M-43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364196..375251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEE84_RS01570 (IEE84_01570) - 365117..366250 (+) 1134 WP_191114663.1 hypothetical protein -
  IEE84_RS01575 (IEE84_01575) - 366238..366609 (+) 372 WP_224737840.1 RnfH family protein -
  IEE84_RS01580 (IEE84_01580) - 366644..367063 (-) 420 WP_191114664.1 outer membrane protein assembly factor BamE -
  IEE84_RS01585 (IEE84_01585) fur 367381..367815 (+) 435 WP_057758249.1 ferric iron uptake transcriptional regulator -
  IEE84_RS01590 (IEE84_01590) pilU 367942..369063 (-) 1122 WP_191114665.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IEE84_RS01595 (IEE84_01595) pilT 369196..370251 (-) 1056 WP_179798169.1 type IV pilus twitching motility protein PilT Machinery gene
  IEE84_RS01600 (IEE84_01600) - 370594..371310 (+) 717 WP_224737843.1 YggS family pyridoxal phosphate-dependent enzyme -
  IEE84_RS01605 (IEE84_01605) - 371357..372631 (-) 1275 WP_057758257.1 hemolysin family protein -
  IEE84_RS01610 (IEE84_01610) - 372844..374535 (-) 1692 WP_057758259.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39216.11 Da        Isoelectric Point: 6.8441

>NTDB_id=484425 IEE84_RS01595 WP_179798169.1 369196..370251(-) (pilT) [Psychrobacter sp. 28M-43]
MAEKNNLNIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPEMTHQVVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFRRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINESRKEH
ILTIEDPIEFVHQSKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQVGGRIAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGAGDGMI
TLDQTLKNLVSKGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=484425 IEE84_RS01595 WP_179798169.1 369196..370251(-) (pilT) [Psychrobacter sp. 28M-43]
ATGGCTGAAAAAAATAATCTTAATATCGAAGACTTGCTGCGCTTTACGGTTAAACATAAGGCATCAGATTTACATATTTC
AGCAGGTATGCCGGCCATGATACGTGTCGATGGTGAAATGACACGTATCAATATGCCAGAAATGACCCATCAAGTTGTGC
ATCAGCTTATTTATGACATTATGAATGATAAGCAACGTGCCGACTTTGAAGAGTTTTTTGAAACGGATTTTTCTTTTGAA
ATTCCTAATCTAGCACGTTTTCGTGTCAACGCTTTTAATCAAAACCGCGGTGCAGGTGCTGTATTTCGTACCATTCCTTC
TAAAGTATTAACGATGGAAGACTTAGGTATGGGCGATGTCTTTAGACGCGTTTCAGACTTTAAGCGTGGTGTGGTATTGG
TCACGGGCCCAACAGGCTCAGGTAAATCGACAACACTTGCGGCGATGATTGACTATATTAACGAAAGCCGTAAAGAGCAT
ATTTTAACTATCGAAGACCCGATTGAATTTGTGCATCAATCTAAGAAAAGCTTGATCAACCAGCGTGAAGTACACCGTGA
TACTTTGGGTTTTGAGCCAGCATTGCGCTCTGCCCTACGTGAGGATCCCGATGTCATCTTAGTTGGTGAGCTACGTGATC
TTGAGACTATCCGCTTAGCGTTGACCGCAGCAGAAACTGGCCACTTAGTATTCGGCACCTTGCATACCAGCTCAGCGGCT
AAAACGATTGACCGTGTTATTGATGTATTTCCTGCCGCTGAAAAAGATATGATCCGCGCTATGCTATCCGAGTCGTTGCA
AGCTGTTATTTCACAGACATTGTTGCGTCGTCAAGTAGGTGGACGTATAGCCGCCCATGAAATCATGATAGGTACCCCTG
CTATCCGTAACTTAATACGTGAGAACAAAATTGCCCAAATGTACTCTGCTATCCAAACAGGTGCAGGCGATGGCATGATT
ACCCTTGATCAGACGTTAAAGAACTTGGTAAGTAAAGGAACTATTAGTAAGGATGTTGCACGTCCTTATGCCAAGCAGCC
TGAGGCATTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.71

98.291

0.783
  pilT Acinetobacter baumannii D1279779

79.42

98.291

0.781
  pilT Acinetobacter baumannii strain A118

79.42

98.291

0.781
  pilT Pseudomonas stutzeri DSM 10701

77.326

98.006

0.758
  pilT Pseudomonas aeruginosa PAK

77.326

98.006

0.758
  pilT Acinetobacter baylyi ADP1

76.453

98.006

0.749
  pilT Legionella pneumophila strain Lp02

71.802

98.006

0.704
  pilT Legionella pneumophila strain ERS1305867

71.802

98.006

0.704
  pilT Neisseria meningitidis 8013

64.928

98.291

0.638
  pilT Neisseria gonorrhoeae MS11

64.348

98.291

0.632
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.869

93.732

0.627
  pilT Vibrio cholerae strain A1552

66.869

93.732

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.856

98.86

0.493
  pilU Acinetobacter baylyi ADP1

41.399

97.721

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.298

96.581

0.399
  pilU Vibrio cholerae strain A1552

42.105

92.023

0.387